Pacific flying fox faeces associated gemycircularvirus-11

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Cyclovirus; unclassified Cyclovirus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTS0|A0A140CTS0_9CIRC RepA OS=Pacific flying fox faeces associated gemycircularvirus-11 OX=1795990 PE=3 SV=1
MM1 pKa = 7.39VPFILKK7 pKa = 9.68KK8 pKa = 9.45VRR10 pKa = 11.84RR11 pKa = 11.84AILTYY16 pKa = 8.51SQCGTLDD23 pKa = 3.61PFTVSDD29 pKa = 3.65HH30 pKa = 6.3FSNLGAEE37 pKa = 4.53CIVGRR42 pKa = 11.84EE43 pKa = 3.85RR44 pKa = 11.84HH45 pKa = 5.69SDD47 pKa = 3.32GGIHH51 pKa = 6.34LHH53 pKa = 5.85VFVDD57 pKa = 4.6FGRR60 pKa = 11.84QFSSRR65 pKa = 11.84KK66 pKa = 7.81TDD68 pKa = 3.1VFDD71 pKa = 3.82VGGYY75 pKa = 8.71PPNIAKK81 pKa = 10.43CGRR84 pKa = 11.84TPWKK88 pKa = 10.45AYY90 pKa = 10.52DD91 pKa = 3.69YY92 pKa = 10.6AIKK95 pKa = 10.92DD96 pKa = 3.52GDD98 pKa = 4.01VVAGGLEE105 pKa = 4.02RR106 pKa = 11.84PIEE109 pKa = 4.15ASGDD113 pKa = 3.55GAGTVDD119 pKa = 4.12PTWHH123 pKa = 6.82SILEE127 pKa = 4.33AEE129 pKa = 4.5TPEE132 pKa = 4.23EE133 pKa = 4.41FWALCHH139 pKa = 7.01DD140 pKa = 4.97LAPRR144 pKa = 11.84DD145 pKa = 3.72LARR148 pKa = 11.84SFPSLQKK155 pKa = 10.22YY156 pKa = 9.88CDD158 pKa = 2.91WRR160 pKa = 11.84YY161 pKa = 9.97RR162 pKa = 11.84PVDD165 pKa = 3.75EE166 pKa = 4.83PHH168 pKa = 6.37RR169 pKa = 11.84TPDD172 pKa = 3.2GYY174 pKa = 11.39QFDD177 pKa = 4.0TSRR180 pKa = 11.84APEE183 pKa = 4.1LNEE186 pKa = 3.56WLLQANLGLRR196 pKa = 11.84QLGLRR201 pKa = 4.19

Molecular weight: 22.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTR9|A0A140CTR9_9CIRC Replication-associated protein OS=Pacific flying fox faeces associated gemycircularvirus-11 OX=1795990 PE=3 SV=1
MM1 pKa = 7.49PRR3 pKa = 11.84YY4 pKa = 9.86ANRR7 pKa = 11.84RR8 pKa = 11.84ISKK11 pKa = 10.11KK12 pKa = 6.42YY13 pKa = 8.64TKK15 pKa = 9.42TKK17 pKa = 8.96RR18 pKa = 11.84KK19 pKa = 8.87PYY21 pKa = 9.61RR22 pKa = 11.84RR23 pKa = 11.84PARR26 pKa = 11.84PSKK29 pKa = 10.66KK30 pKa = 10.22YY31 pKa = 10.44GGKK34 pKa = 9.96SSLKK38 pKa = 10.53KK39 pKa = 9.91MLNLVSTKK47 pKa = 10.34KK48 pKa = 10.15RR49 pKa = 11.84DD50 pKa = 3.49TMLTYY55 pKa = 11.11SNTSATKK62 pKa = 9.39PAGDD66 pKa = 3.68TAYY69 pKa = 10.16GAHH72 pKa = 7.57DD73 pKa = 3.57ATLIGGQSYY82 pKa = 10.08IFPWIATARR91 pKa = 11.84PAITTDD97 pKa = 3.07NNAGFPIDD105 pKa = 3.71QACRR109 pKa = 11.84TASTCYY115 pKa = 9.54MRR117 pKa = 11.84GLKK120 pKa = 9.81EE121 pKa = 4.55RR122 pKa = 11.84ITMQTNTGSAWQWRR136 pKa = 11.84RR137 pKa = 11.84ICFTMKK143 pKa = 10.4GDD145 pKa = 3.72QLYY148 pKa = 8.1STDD151 pKa = 3.35GSNLRR156 pKa = 11.84WSLMANEE163 pKa = 4.41VGGVTGMVRR172 pKa = 11.84VLNSVSGSNAGSTLVDD188 pKa = 4.88LIFRR192 pKa = 11.84GKK194 pKa = 10.47FGQDD198 pKa = 2.75WNNVMSAPLDD208 pKa = 3.59QNLITVKK215 pKa = 10.28YY216 pKa = 9.67DD217 pKa = 3.06KK218 pKa = 10.58TRR220 pKa = 11.84SIQSGNDD227 pKa = 3.22DD228 pKa = 4.45GILRR232 pKa = 11.84TYY234 pKa = 9.64CVWHH238 pKa = 6.56PMAKK242 pKa = 9.96NLVYY246 pKa = 10.74GDD248 pKa = 4.43DD249 pKa = 3.84EE250 pKa = 5.0NGGFEE255 pKa = 3.95DD256 pKa = 3.46TRR258 pKa = 11.84RR259 pKa = 11.84YY260 pKa = 9.63SAGGKK265 pKa = 10.08AGMGDD270 pKa = 4.25YY271 pKa = 10.87YY272 pKa = 11.44VVDD275 pKa = 5.35IINSALGSAPTDD287 pKa = 3.29RR288 pKa = 11.84VNSGDD293 pKa = 3.35KK294 pKa = 10.5LYY296 pKa = 11.25SVLARR301 pKa = 11.84EE302 pKa = 4.69SKK304 pKa = 10.83

Molecular weight: 33.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

829

201

324

276.3

31.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.876 ± 0.324

1.809 ± 0.271

7.479 ± 0.435

3.86 ± 0.95

3.981 ± 0.832

9.047 ± 0.21

2.413 ± 0.736

4.463 ± 0.173

5.308 ± 0.884

7.479 ± 0.624

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.689 ± 0.81

3.257 ± 1.104

5.669 ± 0.863

2.895 ± 0.11

7.358 ± 0.281

7.117 ± 0.712

5.911 ± 1.098

6.152 ± 0.576

2.654 ± 0.334

4.584 ± 0.419
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski