Desulfovibrio ferrophilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Desulfovibrio

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3428 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z6AWE5|A0A2Z6AWE5_9DELT Phosphotransferase system enzyme IIA component OS=Desulfovibrio ferrophilus OX=241368 GN=DFE_0851 PE=4 SV=1
MM1 pKa = 6.32YY2 pKa = 9.45TIEE5 pKa = 3.9ARR7 pKa = 11.84LYY9 pKa = 8.89QKK11 pKa = 10.52IMNDD15 pKa = 2.93QAAQTEE21 pKa = 4.39LLDD24 pKa = 4.51AGNDD28 pKa = 3.55QEE30 pKa = 4.38RR31 pKa = 11.84MEE33 pKa = 4.48VIVRR37 pKa = 11.84QGKK40 pKa = 7.97NHH42 pKa = 6.49GLHH45 pKa = 5.22VTPDD49 pKa = 3.44SVHH52 pKa = 6.2TFLKK56 pKa = 10.87DD57 pKa = 3.33KK58 pKa = 10.77MNTEE62 pKa = 4.23LSDD65 pKa = 3.88DD66 pKa = 3.83QLDD69 pKa = 3.77MVVGGKK75 pKa = 10.0GEE77 pKa = 3.98PASPNDD83 pKa = 3.89VIHH86 pKa = 6.92GNQFLDD92 pKa = 3.62LFGFTLDD99 pKa = 4.28DD100 pKa = 4.02NFDD103 pKa = 3.76GGEE106 pKa = 4.15GNDD109 pKa = 4.09YY110 pKa = 11.0ISGGWGNDD118 pKa = 3.02TLAGGTGNDD127 pKa = 3.44TLYY130 pKa = 11.14GGTGDD135 pKa = 3.64DD136 pKa = 3.52VMRR139 pKa = 11.84GGEE142 pKa = 3.96GHH144 pKa = 7.52DD145 pKa = 3.93YY146 pKa = 11.01MNGGQGNDD154 pKa = 3.39SMTGGQGNDD163 pKa = 3.05TMVGGDD169 pKa = 4.08GHH171 pKa = 8.2DD172 pKa = 3.4EE173 pKa = 4.32MYY175 pKa = 10.98GGTGNDD181 pKa = 3.18NMAGDD186 pKa = 4.27SGNDD190 pKa = 3.27TMYY193 pKa = 11.21GGANSDD199 pKa = 3.63TMDD202 pKa = 4.04GGSGNDD208 pKa = 2.84IMYY211 pKa = 10.32GGSGNDD217 pKa = 3.22SMEE220 pKa = 4.5GGLGDD225 pKa = 3.92DD226 pKa = 4.42LLRR229 pKa = 11.84GEE231 pKa = 5.07EE232 pKa = 4.39GDD234 pKa = 3.5DD235 pKa = 3.72TLRR238 pKa = 11.84GGYY241 pKa = 9.62GQDD244 pKa = 3.03TLYY247 pKa = 11.32GGAGNDD253 pKa = 3.95LLSGGLGSNNLYY265 pKa = 10.69GGEE268 pKa = 4.32GNDD271 pKa = 3.55TLLGGTASTYY281 pKa = 10.09MDD283 pKa = 4.22GGTGNDD289 pKa = 3.73LLVGHH294 pKa = 7.07TGSDD298 pKa = 3.1GMYY301 pKa = 10.48GGDD304 pKa = 4.21GHH306 pKa = 6.69DD307 pKa = 3.52TMYY310 pKa = 11.19GGAGNDD316 pKa = 3.5GMLGEE321 pKa = 5.2AGNDD325 pKa = 3.49YY326 pKa = 10.57MRR328 pKa = 11.84GDD330 pKa = 3.65SGNDD334 pKa = 3.12NMHH337 pKa = 6.66GGSGNDD343 pKa = 3.2TMFGGTGDD351 pKa = 4.29DD352 pKa = 3.93LLSGGEE358 pKa = 4.31GNDD361 pKa = 3.57VLHH364 pKa = 6.91GGAGTDD370 pKa = 3.45TLYY373 pKa = 11.34GGAGNDD379 pKa = 3.95TLWADD384 pKa = 3.52SGGGDD389 pKa = 3.9DD390 pKa = 4.61VLIGGSGADD399 pKa = 2.99VFVIGEE405 pKa = 3.97SDD407 pKa = 3.98GNVTIADD414 pKa = 4.39FNPEE418 pKa = 3.89EE419 pKa = 4.77DD420 pKa = 4.5KK421 pKa = 11.52LGLTNVPANGLVDD434 pKa = 3.28MDD436 pKa = 3.86VTNEE440 pKa = 3.83NGNTIIKK447 pKa = 10.61YY448 pKa = 10.46GDD450 pKa = 3.34TTVTLEE456 pKa = 3.8GVTLSGYY463 pKa = 7.22QVWDD467 pKa = 3.3ILDD470 pKa = 3.67TKK472 pKa = 11.11

Molecular weight: 48.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z6AZQ8|A0A2Z6AZQ8_9DELT Putative transcriptional regulator OS=Desulfovibrio ferrophilus OX=241368 GN=DFE_1954 PE=4 SV=1
MM1 pKa = 7.6PVLLTYY7 pKa = 10.8LPAILSMWLLGAHH20 pKa = 7.36ALRR23 pKa = 11.84GGQPGLMLAWALMPALFLLHH43 pKa = 6.95RR44 pKa = 11.84SWSHH48 pKa = 7.19RR49 pKa = 11.84IGQIALAAGVILWANTGSSLIQIRR73 pKa = 11.84IALDD77 pKa = 3.62QPWTRR82 pKa = 11.84LALILSGLILLAVLGIILLEE102 pKa = 4.17SQRR105 pKa = 11.84VATRR109 pKa = 11.84TQSDD113 pKa = 4.2TGPQWAPAWAFALCAGLLSTTQAKK137 pKa = 10.28VSFPILLPDD146 pKa = 3.8RR147 pKa = 11.84FLPGSGWLLILGLSIYY163 pKa = 10.42AAEE166 pKa = 4.24LTRR169 pKa = 11.84SFLMPGLKK177 pKa = 9.56PRR179 pKa = 11.84QWSTKK184 pKa = 10.02RR185 pKa = 11.84IRR187 pKa = 11.84LWGLFSAVFFVQLILGLAGAEE208 pKa = 3.76RR209 pKa = 11.84MLMTGQLHH217 pKa = 6.36LPIPALIMAGPLYY230 pKa = 10.29RR231 pKa = 11.84GEE233 pKa = 4.33GFFMLILFLSTVALVGPAWCSWLCYY258 pKa = 9.59IGAWDD263 pKa = 4.05GLSSRR268 pKa = 11.84TRR270 pKa = 11.84KK271 pKa = 7.59TPAPLPRR278 pKa = 11.84WRR280 pKa = 11.84QPVRR284 pKa = 11.84LGLFVLVAGTAWGLRR299 pKa = 11.84HH300 pKa = 6.14WGAPISVALALAAGFGLGGVAIMLTLSRR328 pKa = 11.84RR329 pKa = 11.84KK330 pKa = 9.94GVMVHH335 pKa = 6.05CTTFCPIGLLANILGKK351 pKa = 10.6LNPFRR356 pKa = 11.84VRR358 pKa = 11.84ITEE361 pKa = 4.0GCTGCGVCAAVCRR374 pKa = 11.84YY375 pKa = 9.25DD376 pKa = 4.23ALDD379 pKa = 3.58EE380 pKa = 4.04EE381 pKa = 5.49RR382 pKa = 11.84IALGKK387 pKa = 9.28PHH389 pKa = 7.5LSCTLCGDD397 pKa = 4.2CVGSCPHH404 pKa = 6.41TAMHH408 pKa = 6.23YY409 pKa = 8.89VFPGLSNANARR420 pKa = 11.84ALFVTMAVAAHH431 pKa = 6.21AVFLGVARR439 pKa = 11.84LL440 pKa = 3.49

Molecular weight: 47.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3428

0

3428

1088601

43

2103

317.6

34.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.55 ± 0.047

1.291 ± 0.021

5.739 ± 0.04

6.524 ± 0.04

3.938 ± 0.025

8.146 ± 0.056

2.113 ± 0.021

5.462 ± 0.034

4.619 ± 0.041

10.52 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.979 ± 0.021

3.131 ± 0.025

4.627 ± 0.034

3.424 ± 0.027

5.878 ± 0.034

5.751 ± 0.031

5.301 ± 0.026

7.223 ± 0.037

1.194 ± 0.016

2.591 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski