Colletotrichum higginsianum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta;

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12820 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4T0VSF7|A0A4T0VSF7_9PEZI Ankyrin repeat-containing protein OS=Colletotrichum higginsianum OX=80884 GN=CH35J_008908 PE=4 SV=1
MM1 pKa = 7.4SRR3 pKa = 11.84TPNRR7 pKa = 11.84PTSGGLDD14 pKa = 3.64FNNSAGCDD22 pKa = 3.62YY23 pKa = 11.55ASATTTSSSSASSFSSSTSSAANINLDD50 pKa = 4.11IDD52 pKa = 4.48DD53 pKa = 5.18DD54 pKa = 4.84NINKK58 pKa = 8.23STAAAAATTTTTTNTDD74 pKa = 4.18FYY76 pKa = 9.84TSSASTSATNDD87 pKa = 2.82SSEE90 pKa = 4.11GFNFSCPSTTSSSSSSSNNAA110 pKa = 3.06

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4T0VHG4|A0A4T0VHG4_9PEZI Major facilitator superfamily domain-containing protein 1 OS=Colletotrichum higginsianum OX=80884 GN=CH35J_010626 PE=4 SV=1
MM1 pKa = 7.85PLRR4 pKa = 11.84STRR7 pKa = 11.84HH8 pKa = 4.18TTTTAAPRR16 pKa = 11.84RR17 pKa = 11.84TGLFSRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84AAPTTSRR32 pKa = 11.84HH33 pKa = 4.45HH34 pKa = 6.15HH35 pKa = 4.81TTTRR39 pKa = 11.84TTRR42 pKa = 11.84TTGTTARR49 pKa = 11.84PAKK52 pKa = 10.26RR53 pKa = 11.84GGLFSRR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84GPVTTHH67 pKa = 7.1AAPVHH72 pKa = 4.77HH73 pKa = 6.24QRR75 pKa = 11.84RR76 pKa = 11.84KK77 pKa = 9.81PSMGDD82 pKa = 3.3KK83 pKa = 10.53ISGAMLKK90 pKa = 10.72LKK92 pKa = 9.36GTLTRR97 pKa = 11.84RR98 pKa = 11.84PGQKK102 pKa = 9.9AAGTRR107 pKa = 11.84RR108 pKa = 11.84MHH110 pKa = 5.68GTDD113 pKa = 2.92GRR115 pKa = 11.84GSRR118 pKa = 11.84RR119 pKa = 11.84AYY121 pKa = 10.23RR122 pKa = 11.84YY123 pKa = 9.61

Molecular weight: 13.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12820

0

12820

6257554

59

12530

488.1

53.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.456 ± 0.021

1.214 ± 0.009

5.849 ± 0.016

5.989 ± 0.022

3.752 ± 0.012

7.348 ± 0.025

2.31 ± 0.01

4.51 ± 0.016

4.598 ± 0.021

8.741 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.182 ± 0.01

3.514 ± 0.014

6.085 ± 0.028

3.824 ± 0.015

6.149 ± 0.022

7.758 ± 0.024

5.956 ± 0.031

6.548 ± 0.02

1.53 ± 0.009

2.688 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski