Aminipila sp. CBA3637

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Eubacteriales Family XIII. Incertae Sedis; Aminipila

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3071 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P1MHK4|A0A6P1MHK4_9FIRM TetR family transcriptional regulator OS=Aminipila sp. CBA3637 OX=2697030 GN=Ami3637_15185 PE=4 SV=1
MM1 pKa = 7.53QEE3 pKa = 4.32DD4 pKa = 3.72SSIFDD9 pKa = 3.45GLRR12 pKa = 11.84EE13 pKa = 4.01KK14 pKa = 10.82EE15 pKa = 3.83YY16 pKa = 10.66GYY18 pKa = 11.02CKK20 pKa = 10.55NEE22 pKa = 3.73EE23 pKa = 4.12LATLGVLAMLPVDD36 pKa = 3.97IDD38 pKa = 3.81SLINDD43 pKa = 4.72VIQVDD48 pKa = 4.28NYY50 pKa = 10.99LSTISLIVEE59 pKa = 4.0QQAA62 pKa = 3.17

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P1MK75|A0A6P1MK75_9FIRM NADPH-dependent FMN reductase OS=Aminipila sp. CBA3637 OX=2697030 GN=Ami3637_15190 PE=4 SV=1
MM1 pKa = 7.5DD2 pKa = 4.0TKK4 pKa = 11.12LKK6 pKa = 10.83NFSNNTAIRR15 pKa = 11.84ILTLIMCVMLFTATLIGSIATVIGLQKK42 pKa = 10.45AGRR45 pKa = 11.84SFTLDD50 pKa = 3.5EE51 pKa = 4.29ILVLRR56 pKa = 11.84ITLKK60 pKa = 10.3VVIYY64 pKa = 9.35RR65 pKa = 11.84KK66 pKa = 9.86NSIRR70 pKa = 11.84ILQRR74 pKa = 11.84FYY76 pKa = 10.93II77 pKa = 4.23

Molecular weight: 8.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3071

0

3071

934980

26

2887

304.5

34.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.987 ± 0.046

1.363 ± 0.019

5.448 ± 0.038

6.985 ± 0.06

4.188 ± 0.035

6.92 ± 0.049

1.508 ± 0.019

8.57 ± 0.048

7.719 ± 0.043

8.762 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.005 ± 0.026

5.382 ± 0.038

3.063 ± 0.027

3.163 ± 0.024

3.503 ± 0.033

6.284 ± 0.046

5.579 ± 0.051

6.734 ± 0.034

0.796 ± 0.013

4.04 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski