Helicobacter sp. MIT 01-6242

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter; unclassified Helicobacter

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1458 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1U655|A0A1B1U655_9HELI Transcription termination/antitermination protein NusA OS=Helicobacter sp. MIT 01-6242 OX=222136 GN=nusA PE=3 SV=1
MM1 pKa = 7.53AVMIGDD7 pKa = 3.64EE8 pKa = 5.1CIACDD13 pKa = 3.89ACRR16 pKa = 11.84EE17 pKa = 4.11EE18 pKa = 5.02CPNEE22 pKa = 4.29AIEE25 pKa = 4.63EE26 pKa = 4.28GDD28 pKa = 3.98PIYY31 pKa = 11.09SVDD34 pKa = 3.53PDD36 pKa = 3.63RR37 pKa = 11.84CTEE40 pKa = 4.14CIGYY44 pKa = 9.79YY45 pKa = 10.15DD46 pKa = 4.65EE47 pKa = 4.61PTCISVCPVDD57 pKa = 5.94AISADD62 pKa = 3.84PDD64 pKa = 3.41NMEE67 pKa = 5.24SIEE70 pKa = 3.93EE71 pKa = 4.03LKK73 pKa = 10.92FKK75 pKa = 10.94FEE77 pKa = 3.98QLQKK81 pKa = 11.51GEE83 pKa = 4.02

Molecular weight: 9.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1U4H3|A0A1B1U4H3_9HELI ATP-binding protein OS=Helicobacter sp. MIT 01-6242 OX=222136 GN=BBW65_01785 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.31RR14 pKa = 11.84THH16 pKa = 5.88GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.07TKK25 pKa = 10.39NGRR28 pKa = 11.84KK29 pKa = 9.3VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.41GRR39 pKa = 11.84KK40 pKa = 8.68RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1458

0

1458

471126

37

2881

323.1

36.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.923 ± 0.057

1.274 ± 0.037

4.864 ± 0.039

6.399 ± 0.079

5.099 ± 0.063

6.805 ± 0.092

2.006 ± 0.035

8.048 ± 0.058

7.299 ± 0.059

10.385 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.159 ± 0.035

5.159 ± 0.108

3.235 ± 0.045

4.733 ± 0.045

3.693 ± 0.049

6.781 ± 0.059

5.2 ± 0.139

5.589 ± 0.058

0.754 ± 0.023

3.593 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski