Variibacter gotjawalensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Variibacter

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4437 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S3PP12|A0A0S3PP12_9BRAD Putative diguanylate cyclase YedQ OS=Variibacter gotjawalensis OX=1333996 GN=yedQ_1 PE=4 SV=1
MM1 pKa = 7.55ARR3 pKa = 11.84TGTNSADD10 pKa = 3.47VIYY13 pKa = 10.62DD14 pKa = 3.41SGLGEE19 pKa = 4.28TIYY22 pKa = 10.41TYY24 pKa = 11.15GGNDD28 pKa = 3.25VIYY31 pKa = 10.55AGLGHH36 pKa = 6.48DD37 pKa = 4.66TIDD40 pKa = 4.42AGAGDD45 pKa = 4.39DD46 pKa = 4.23TIMGSSQTSSVTMAWGYY63 pKa = 11.08DD64 pKa = 3.64YY65 pKa = 11.72VVGGQGNDD73 pKa = 3.57YY74 pKa = 10.57INYY77 pKa = 10.13SMTTNEE83 pKa = 4.0VIIYY87 pKa = 9.9GDD89 pKa = 3.63DD90 pKa = 3.63VNTHH94 pKa = 5.63VLPGNDD100 pKa = 3.62YY101 pKa = 10.86IVGGRR106 pKa = 11.84QSDD109 pKa = 3.81TLIGGGGDD117 pKa = 3.55DD118 pKa = 5.24QIWGGNGGDD127 pKa = 4.59AIYY130 pKa = 10.82GDD132 pKa = 4.07AVLYY136 pKa = 10.17IQGGNDD142 pKa = 2.96RR143 pKa = 11.84LFGEE147 pKa = 5.12GGNDD151 pKa = 3.72TISGGFGNDD160 pKa = 4.43LINGGAGKK168 pKa = 10.06DD169 pKa = 3.65VLIGGPGTDD178 pKa = 2.37WFVFDD183 pKa = 5.18TIGDD187 pKa = 4.03SLSFYY192 pKa = 10.1ATADD196 pKa = 3.52VIKK199 pKa = 10.88DD200 pKa = 3.6FDD202 pKa = 4.04RR203 pKa = 11.84TSDD206 pKa = 3.87WIVMNHH212 pKa = 6.61AGNSGNYY219 pKa = 9.42KK220 pKa = 9.74EE221 pKa = 4.8FAFSGSTNAEE231 pKa = 3.81ANFNKK236 pKa = 9.48ALQMASQVIHH246 pKa = 5.77QVDD249 pKa = 4.4FVFVTDD255 pKa = 3.52GHH257 pKa = 6.56DD258 pKa = 3.58GYY260 pKa = 11.62LFADD264 pKa = 3.68QDD266 pKa = 3.91RR267 pKa = 11.84NSTVDD272 pKa = 3.15TGVVLMGVDD281 pKa = 3.91SLSKK285 pKa = 10.45FQATDD290 pKa = 2.54IMVFF294 pKa = 3.54

Molecular weight: 31.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S3PRK2|A0A0S3PRK2_9BRAD DNA polymerase IV OS=Variibacter gotjawalensis OX=1333996 GN=GJW-30_1_01047 PE=4 SV=1
MM1 pKa = 7.43AAKK4 pKa = 10.21KK5 pKa = 8.82ATRR8 pKa = 11.84KK9 pKa = 10.03KK10 pKa = 9.18PVRR13 pKa = 11.84RR14 pKa = 11.84AAAKK18 pKa = 9.99KK19 pKa = 8.18ATARR23 pKa = 11.84KK24 pKa = 5.44TTAKK28 pKa = 10.1RR29 pKa = 11.84KK30 pKa = 8.34PAAKK34 pKa = 8.67KK35 pKa = 7.24TAKK38 pKa = 9.01KK39 pKa = 5.85TTRR42 pKa = 11.84KK43 pKa = 9.1VAKK46 pKa = 10.03KK47 pKa = 9.31PAAKK51 pKa = 8.73KK52 pKa = 6.52TAKK55 pKa = 10.04RR56 pKa = 11.84AAPKK60 pKa = 10.32RR61 pKa = 11.84KK62 pKa = 7.24TAAKK66 pKa = 8.34KK67 pKa = 6.39TTAKK71 pKa = 10.21KK72 pKa = 10.43ASAKK76 pKa = 9.79KK77 pKa = 10.12AVRR80 pKa = 11.84KK81 pKa = 10.01APAKK85 pKa = 10.11KK86 pKa = 9.69KK87 pKa = 10.14AAAKK91 pKa = 9.66RR92 pKa = 11.84KK93 pKa = 7.16PAKK96 pKa = 9.65AARR99 pKa = 11.84ARR101 pKa = 11.84VVKK104 pKa = 9.44KK105 pKa = 7.88TAPRR109 pKa = 11.84KK110 pKa = 7.97AAKK113 pKa = 9.93RR114 pKa = 11.84KK115 pKa = 9.87APAKK119 pKa = 8.8KK120 pKa = 7.91TAAIQPMGWLEE131 pKa = 4.08SAASLLKK138 pKa = 10.65KK139 pKa = 10.61

Molecular weight: 14.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4437

0

4437

1358188

29

1888

306.1

33.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.522 ± 0.05

0.795 ± 0.011

5.481 ± 0.028

5.443 ± 0.032

3.836 ± 0.023

8.437 ± 0.032

1.936 ± 0.019

5.488 ± 0.025

4.014 ± 0.033

9.548 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.018

2.794 ± 0.024

5.215 ± 0.031

3.122 ± 0.021

6.886 ± 0.033

5.295 ± 0.022

5.497 ± 0.025

7.545 ± 0.027

1.336 ± 0.014

2.316 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski