Paucibacter sp. KBW04

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Paucibacter; unclassified Paucibacter

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4784 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N7C7W3|A0A3N7C7W3_9BURK Flagellin OS=Paucibacter sp. KBW04 OX=2153361 GN=DBR47_18035 PE=3 SV=1
MM1 pKa = 7.59CLICGWIYY9 pKa = 11.2DD10 pKa = 4.08EE11 pKa = 4.96AAGDD15 pKa = 4.19PEE17 pKa = 5.37HH18 pKa = 7.1GIAPGTVWADD28 pKa = 2.55VDD30 pKa = 4.37MNWTCPEE37 pKa = 3.86CGARR41 pKa = 11.84KK42 pKa = 9.87DD43 pKa = 3.6DD44 pKa = 3.99FEE46 pKa = 4.37MVRR49 pKa = 11.84II50 pKa = 4.15

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N7BW92|A0A3N7BW92_9BURK BadM/Rrf2 family transcriptional regulator OS=Paucibacter sp. KBW04 OX=2153361 GN=DBR47_16525 PE=4 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84GGRR5 pKa = 11.84HH6 pKa = 6.14RR7 pKa = 11.84GGQXGGGGSWALGGRR22 pKa = 11.84LGLGGVKK29 pKa = 10.28LLSRR33 pKa = 11.84VANWALPGWVPALARR48 pKa = 11.84SVVLGVGGRR57 pKa = 11.84RR58 pKa = 11.84GPVFPP63 pKa = 5.67

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4784

0

4784

1670315

29

5506

349.1

37.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.906 ± 0.055

0.916 ± 0.013

4.821 ± 0.027

5.483 ± 0.036

3.463 ± 0.023

8.171 ± 0.036

2.062 ± 0.017

4.029 ± 0.02

3.478 ± 0.035

11.944 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.328 ± 0.017

2.7 ± 0.025

5.068 ± 0.028

4.893 ± 0.033

6.587 ± 0.035

6.206 ± 0.043

4.482 ± 0.028

6.777 ± 0.032

1.549 ± 0.016

2.136 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski