Pedobacter sp. RP-1-13

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter; unclassified Pedobacter

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4698 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R0MTC8|A0A4R0MTC8_9SPHI Ankyrin repeat domain-containing protein OS=Pedobacter sp. RP-1-13 OX=2530452 GN=EZ428_13675 PE=4 SV=1
MM1 pKa = 7.19YY2 pKa = 7.77WTLEE6 pKa = 4.21LASHH10 pKa = 7.01LEE12 pKa = 4.08DD13 pKa = 4.93APWPATKK20 pKa = 10.47DD21 pKa = 3.38EE22 pKa = 5.13LIDD25 pKa = 3.42YY26 pKa = 7.68GIRR29 pKa = 11.84SGAPVEE35 pKa = 4.53VIEE38 pKa = 4.56NLQALEE44 pKa = 4.69DD45 pKa = 4.28DD46 pKa = 4.28GEE48 pKa = 4.35PYY50 pKa = 9.33EE51 pKa = 4.57TIEE54 pKa = 4.84EE55 pKa = 4.08IWPDD59 pKa = 3.97YY60 pKa = 7.47PTKK63 pKa = 10.94DD64 pKa = 3.05DD65 pKa = 4.15FFFNEE70 pKa = 4.51DD71 pKa = 3.17EE72 pKa = 4.46YY73 pKa = 11.99

Molecular weight: 8.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R0MPC2|A0A4R0MPC2_9SPHI YCII domain-containing protein OS=Pedobacter sp. RP-1-13 OX=2530452 GN=EZ428_18750 PE=3 SV=1
MM1 pKa = 7.41RR2 pKa = 11.84QLSFFTEE9 pKa = 4.61AGQSARR15 pKa = 11.84LPIEE19 pKa = 3.66VLAYY23 pKa = 10.11HH24 pKa = 6.07PTIIEE29 pKa = 4.3DD30 pKa = 3.92SLGHH34 pKa = 5.61EE35 pKa = 5.39LIDD38 pKa = 3.79AVPAAVRR45 pKa = 11.84LPAWKK50 pKa = 9.04TSLLAAAAIIGNHH63 pKa = 7.54LITKK67 pKa = 9.67IYY69 pKa = 9.69LVKK72 pKa = 10.32ILRR75 pKa = 11.84LGKK78 pKa = 10.11NSDD81 pKa = 3.17YY82 pKa = 11.02RR83 pKa = 11.84AIARR87 pKa = 11.84LFLPLVQWQPSFPLKK102 pKa = 10.06TPSNPLPCHH111 pKa = 6.02NRR113 pKa = 11.84SQRR116 pKa = 11.84HH117 pKa = 4.79RR118 pKa = 11.84PSGRR122 pKa = 11.84GWKK125 pKa = 9.78VIYY128 pKa = 10.02RR129 pKa = 11.84LRR131 pKa = 11.84SII133 pKa = 4.47

Molecular weight: 15.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4698

0

4698

1709408

25

3376

363.9

40.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.239 ± 0.035

0.734 ± 0.01

5.29 ± 0.021

5.618 ± 0.037

5.063 ± 0.025

6.725 ± 0.033

1.616 ± 0.016

7.57 ± 0.033

7.802 ± 0.038

9.413 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.176 ± 0.017

6.244 ± 0.039

3.634 ± 0.018

3.542 ± 0.02

3.451 ± 0.022

6.379 ± 0.031

5.962 ± 0.042

6.172 ± 0.025

1.196 ± 0.015

4.173 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski