Halomonas axialensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5488 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A455VCS9|A0A455VCS9_9GAMM Uncharacterized protein OS=Halomonas axialensis OX=115555 GN=HAALTHF_31320n PE=4 SV=2
MM1 pKa = 6.98TRR3 pKa = 11.84SNRR6 pKa = 11.84SALKK10 pKa = 10.32SLSLAPLTAALLASSLTITPTQAQEE35 pKa = 3.78RR36 pKa = 11.84SDD38 pKa = 5.84FSVCWSIYY46 pKa = 9.54AGWMPWAYY54 pKa = 10.59ADD56 pKa = 4.27VEE58 pKa = 4.6GVVNKK63 pKa = 9.46WADD66 pKa = 3.48EE67 pKa = 4.03YY68 pKa = 11.37GISIDD73 pKa = 3.57VVQVNDD79 pKa = 3.58YY80 pKa = 10.95VEE82 pKa = 5.1SINQFTSGNVDD93 pKa = 3.21GCAMTNMDD101 pKa = 5.17ALTIPAASGVDD112 pKa = 3.58STALIINDD120 pKa = 3.71YY121 pKa = 11.52SNGNDD126 pKa = 4.06GIVLKK131 pKa = 11.0GSDD134 pKa = 4.6DD135 pKa = 3.87LADD138 pKa = 3.16IAGRR142 pKa = 11.84QVNLVQFSVSHH153 pKa = 5.55YY154 pKa = 8.95FLARR158 pKa = 11.84ALEE161 pKa = 4.48SVGLTEE167 pKa = 5.89RR168 pKa = 11.84DD169 pKa = 3.11IEE171 pKa = 4.52TVNTSDD177 pKa = 3.85ADD179 pKa = 3.65IVGLFASDD187 pKa = 3.16TTEE190 pKa = 6.07AVAAWNPQLGAIEE203 pKa = 5.34DD204 pKa = 3.95MADD207 pKa = 3.41SNVVYY212 pKa = 9.71TSAEE216 pKa = 3.85IPGEE220 pKa = 4.05ILDD223 pKa = 3.75MMVVNTQTLADD234 pKa = 3.97NPALGHH240 pKa = 6.36ALVGAWYY247 pKa = 9.64EE248 pKa = 3.99VMGLIEE254 pKa = 5.73ADD256 pKa = 3.96DD257 pKa = 4.17EE258 pKa = 4.42NALGIMADD266 pKa = 3.6AAGTDD271 pKa = 2.98IDD273 pKa = 5.15GYY275 pKa = 9.8RR276 pKa = 11.84QQLAATYY283 pKa = 9.81LYY285 pKa = 9.65TDD287 pKa = 3.81PAEE290 pKa = 5.18AIALMEE296 pKa = 4.44SPEE299 pKa = 4.85LLDD302 pKa = 3.07TMQRR306 pKa = 11.84VAEE309 pKa = 4.39FSFQHH314 pKa = 6.2GLLGDD319 pKa = 3.89MAPNAEE325 pKa = 4.31VVGIEE330 pKa = 4.22TPSGVFGDD338 pKa = 3.95EE339 pKa = 4.5SNVQLRR345 pKa = 11.84FDD347 pKa = 3.75PSFTQAYY354 pKa = 10.42LDD356 pKa = 3.75AQQ358 pKa = 3.73

Molecular weight:
38.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A510J396|A0A510J396_9GAMM Ni_hydr_CYTB domain-containing protein OS=Halomonas axialensis OX=115555 GN=HAALTHF_16550n PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84AHH16 pKa = 6.12GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.33NGRR28 pKa = 11.84AVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.12 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5488

0

5488

1002324

39

1404

182.6

20.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.866 ± 0.045

1.104 ± 0.013

5.257 ± 0.035

6.097 ± 0.038

3.438 ± 0.027

7.696 ± 0.032

2.468 ± 0.019

4.847 ± 0.029

3.198 ± 0.029

11.014 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.811 ± 0.022

2.887 ± 0.024

4.969 ± 0.028

4.384 ± 0.027

6.725 ± 0.036

5.856 ± 0.027

5.286 ± 0.026

7.082 ± 0.034

1.555 ± 0.019

2.461 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski