Staphylococcus massiliensis S46

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Staphylococcus; Staphylococcus massiliensis

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2323 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9B1Q3|K9B1Q3_9STAP 50S ribosomal subunit assembly factor BipA OS=Staphylococcus massiliensis S46 OX=1229783 GN=bipA PE=3 SV=1
MM1 pKa = 7.74EE2 pKa = 6.76DD3 pKa = 2.57IHH5 pKa = 9.03KK6 pKa = 10.63IIDD9 pKa = 4.78DD10 pKa = 3.97INLQNLKK17 pKa = 10.82GIDD20 pKa = 3.64SRR22 pKa = 11.84VEE24 pKa = 3.6NALTEE29 pKa = 4.22EE30 pKa = 4.14QDD32 pKa = 3.24EE33 pKa = 4.54GALFILGEE41 pKa = 4.07TLYY44 pKa = 10.7QYY46 pKa = 11.82GLIPQGLEE54 pKa = 3.68VFRR57 pKa = 11.84FLYY60 pKa = 10.46HH61 pKa = 6.96KK62 pKa = 10.37FPNEE66 pKa = 3.98PEE68 pKa = 3.78LLIYY72 pKa = 10.14FVEE75 pKa = 4.28GLIAEE80 pKa = 4.64GEE82 pKa = 4.02TDD84 pKa = 3.26EE85 pKa = 4.49ALEE88 pKa = 3.96YY89 pKa = 9.72LTEE92 pKa = 4.48VEE94 pKa = 4.53DD95 pKa = 4.25TPEE98 pKa = 4.52KK99 pKa = 11.02LMLEE103 pKa = 4.09ADD105 pKa = 5.01LYY107 pKa = 10.32QQLNMLEE114 pKa = 4.15VAIEE118 pKa = 4.13KK119 pKa = 10.46LNQAKK124 pKa = 9.62MIQPEE129 pKa = 3.93DD130 pKa = 4.27PIINFALAEE139 pKa = 3.99LFYY142 pKa = 11.38YY143 pKa = 10.49DD144 pKa = 4.25GQWLRR149 pKa = 11.84AVTEE153 pKa = 4.01YY154 pKa = 9.92DD155 pKa = 3.54TVLEE159 pKa = 4.46TGDD162 pKa = 3.57YY163 pKa = 9.37MINGVNLFSRR173 pKa = 11.84IADD176 pKa = 3.53CSLQSGNYY184 pKa = 9.51ADD186 pKa = 6.1AIQNYY191 pKa = 10.08DD192 pKa = 4.08EE193 pKa = 5.31ISDD196 pKa = 4.11SEE198 pKa = 4.45MTSEE202 pKa = 5.62DD203 pKa = 3.93YY204 pKa = 10.51FKK206 pKa = 11.15KK207 pKa = 10.62AISYY211 pKa = 7.54EE212 pKa = 4.05KK213 pKa = 10.86NEE215 pKa = 4.12RR216 pKa = 11.84TKK218 pKa = 10.94EE219 pKa = 4.12AISIMKK225 pKa = 10.15DD226 pKa = 3.43LLTKK230 pKa = 10.85DD231 pKa = 3.24PDD233 pKa = 4.61FIQGYY238 pKa = 9.23FYY240 pKa = 10.89LQQLYY245 pKa = 9.6EE246 pKa = 4.21LEE248 pKa = 4.15KK249 pKa = 10.31DD250 pKa = 3.7YY251 pKa = 11.29PSAIEE256 pKa = 4.27IGNEE260 pKa = 3.94GLKK263 pKa = 10.81LNQFYY268 pKa = 10.87KK269 pKa = 11.05EE270 pKa = 4.26LMCQTGSLEE279 pKa = 4.72IEE281 pKa = 4.87HH282 pKa = 7.27GDD284 pKa = 3.47QNDD287 pKa = 4.48GVDD290 pKa = 4.18HH291 pKa = 6.73LLEE294 pKa = 4.33SLEE297 pKa = 4.06VDD299 pKa = 3.92PSYY302 pKa = 11.15HH303 pKa = 6.18EE304 pKa = 4.16PVLILSEE311 pKa = 4.69FYY313 pKa = 10.64RR314 pKa = 11.84EE315 pKa = 4.11QDD317 pKa = 3.14DD318 pKa = 4.07HH319 pKa = 6.67EE320 pKa = 4.75ALIGLIKK327 pKa = 10.89YY328 pKa = 10.08VDD330 pKa = 4.32EE331 pKa = 4.74EE332 pKa = 4.58DD333 pKa = 3.57MDD335 pKa = 4.62PVFMWHH341 pKa = 6.75LAYY344 pKa = 10.07AYY346 pKa = 10.33GEE348 pKa = 4.13EE349 pKa = 4.36EE350 pKa = 3.88RR351 pKa = 11.84DD352 pKa = 3.25KK353 pKa = 11.48EE354 pKa = 4.44AMHH357 pKa = 7.03FYY359 pKa = 10.43QLAYY363 pKa = 8.84QTLNDD368 pKa = 3.72QAPFLSDD375 pKa = 3.34YY376 pKa = 10.69YY377 pKa = 10.94FYY379 pKa = 10.76LIEE382 pKa = 5.48IGHH385 pKa = 6.46IEE387 pKa = 3.99EE388 pKa = 5.16AKK390 pKa = 10.68PILQKK395 pKa = 10.87LKK397 pKa = 10.78EE398 pKa = 3.88IDD400 pKa = 3.45ATNEE404 pKa = 3.58TWFEE408 pKa = 4.06EE409 pKa = 4.15EE410 pKa = 6.06DD411 pKa = 3.52RR412 pKa = 11.84LQQ414 pKa = 4.27

Molecular weight: 48.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9B413|K9B413_9STAP Competence protein F OS=Staphylococcus massiliensis S46 OX=1229783 GN=C273_04870 PE=4 SV=1
MM1 pKa = 7.49VKK3 pKa = 9.1RR4 pKa = 11.84TYY6 pKa = 10.35QPNKK10 pKa = 8.16RR11 pKa = 11.84KK12 pKa = 9.54HH13 pKa = 5.99SKK15 pKa = 8.87VHH17 pKa = 5.77GFRR20 pKa = 11.84KK21 pKa = 10.02RR22 pKa = 11.84MSTKK26 pKa = 10.18NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2323

0

2323

680527

18

3233

293.0

33.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.187 ± 0.05

0.639 ± 0.016

6.124 ± 0.071

7.032 ± 0.064

4.494 ± 0.042

6.281 ± 0.062

2.44 ± 0.03

8.258 ± 0.069

7.473 ± 0.064

9.317 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.029

5.049 ± 0.056

3.328 ± 0.041

3.759 ± 0.042

3.759 ± 0.047

5.994 ± 0.05

5.723 ± 0.084

6.836 ± 0.047

0.723 ± 0.018

3.838 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski