Rhodopirellula sallentina SM41

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Rhodopirellula; Rhodopirellula sallentina

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6872 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M5U4W3|M5U4W3_9BACT TPR repeat-containing protein OS=Rhodopirellula sallentina SM41 OX=1263870 GN=RSSM_05710 PE=4 SV=1
MM1 pKa = 6.45FQKK4 pKa = 10.5VVSKK8 pKa = 9.12WVAPIVCLAVMAVNANAGSPTSLADD33 pKa = 6.17DD34 pKa = 3.67SDD36 pKa = 3.68QYY38 pKa = 11.09NAYY41 pKa = 8.97VYY43 pKa = 10.41SYY45 pKa = 11.49VSMVYY50 pKa = 10.73ADD52 pKa = 5.2GISNVIGDD60 pKa = 4.48DD61 pKa = 3.66SLEE64 pKa = 4.36GEE66 pKa = 4.48VAILISGFCSDD77 pKa = 5.07GFFHH81 pKa = 7.33CFDD84 pKa = 4.71ALVDD88 pKa = 4.69DD89 pKa = 6.66DD90 pKa = 4.12DD91 pKa = 4.99TLWQDD96 pKa = 3.65AVDD99 pKa = 4.23DD100 pKa = 4.51LEE102 pKa = 4.86LARR105 pKa = 11.84MWCDD109 pKa = 3.17TLIVFVEE116 pKa = 4.53DD117 pKa = 3.54SQPASVSGQIEE128 pKa = 4.19TLMWYY133 pKa = 10.62LDD135 pKa = 3.42IAIANAKK142 pKa = 10.26DD143 pKa = 3.83CVPPTRR149 pKa = 11.84LWRR152 pKa = 11.84LPLQTSVKK160 pKa = 9.6RR161 pKa = 11.84WW162 pKa = 3.15

Molecular weight: 17.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M5U9H2|M5U9H2_9BACT OsmC family protein OS=Rhodopirellula sallentina SM41 OX=1263870 GN=RSSM_00584 PE=4 SV=1
MM1 pKa = 7.46TLPTPRR7 pKa = 11.84VKK9 pKa = 10.33SRR11 pKa = 11.84KK12 pKa = 8.64RR13 pKa = 11.84SRR15 pKa = 11.84SQASRR20 pKa = 11.84FSRR23 pKa = 11.84DD24 pKa = 3.17NLFVTLSFTAVRR36 pKa = 11.84PSSRR40 pKa = 11.84QSLFGSAFFAVRR52 pKa = 11.84SQRR55 pKa = 11.84RR56 pKa = 11.84SNPSRR61 pKa = 3.45

Molecular weight: 7.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6872

0

6872

2353469

34

8027

342.5

37.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.402 ± 0.031

1.131 ± 0.013

6.561 ± 0.035

6.272 ± 0.03

3.649 ± 0.018

7.699 ± 0.036

2.225 ± 0.022

5.06 ± 0.023

3.415 ± 0.034

9.093 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.311 ± 0.02

3.419 ± 0.025

5.174 ± 0.027

3.802 ± 0.017

6.811 ± 0.039

7.038 ± 0.031

5.86 ± 0.044

7.226 ± 0.028

1.474 ± 0.016

2.378 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski