Serratia phage CBH8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Winklervirus; unclassified Winklervirus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 275 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z1LYB1|A0A1Z1LYB1_9CAUD Uncharacterized protein OS=Serratia phage CBH8 OX=2006940 PE=4 SV=1
MM1 pKa = 7.34NIEE4 pKa = 4.01VKK6 pKa = 10.38KK7 pKa = 10.57IYY9 pKa = 10.49AEE11 pKa = 4.97SGDD14 pKa = 4.31CDD16 pKa = 4.11LGCWNSADD24 pKa = 4.14GYY26 pKa = 10.87LVTIDD31 pKa = 3.35GVEE34 pKa = 3.91YY35 pKa = 10.88DD36 pKa = 4.48DD37 pKa = 4.45LRR39 pKa = 11.84PYY41 pKa = 11.0ASCCNSDD48 pKa = 3.75SFNEE52 pKa = 3.86GDD54 pKa = 3.91LLNFIMSKK62 pKa = 10.16VGRR65 pKa = 11.84TDD67 pKa = 2.69IHH69 pKa = 8.34INFEE73 pKa = 4.26DD74 pKa = 3.37

Molecular weight: 8.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z1LYB2|A0A1Z1LYB2_9CAUD Uncharacterized protein OS=Serratia phage CBH8 OX=2006940 PE=4 SV=1
MM1 pKa = 6.82KK2 pKa = 9.39TWVSYY7 pKa = 10.14GRR9 pKa = 11.84PHH11 pKa = 7.18PRR13 pKa = 11.84TGRR16 pKa = 11.84RR17 pKa = 11.84WYY19 pKa = 10.8LEE21 pKa = 3.84AVCRR25 pKa = 11.84EE26 pKa = 3.57TGFRR30 pKa = 11.84VNGKK34 pKa = 7.93FACKK38 pKa = 8.32PTKK41 pKa = 9.93KK42 pKa = 9.9QLRR45 pKa = 11.84KK46 pKa = 7.74FKK48 pKa = 10.2RR49 pKa = 11.84WARR52 pKa = 11.84HH53 pKa = 4.23QIEE56 pKa = 4.81FKK58 pKa = 10.87LYY60 pKa = 8.73WDD62 pKa = 5.49AII64 pKa = 4.13

Molecular weight: 7.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

275

0

275

53392

22

1256

194.2

22.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.876 ± 0.167

1.09 ± 0.063

6.374 ± 0.104

7.288 ± 0.147

4.358 ± 0.103

6.312 ± 0.162

1.877 ± 0.074

6.935 ± 0.122

7.595 ± 0.171

7.548 ± 0.129

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.941 ± 0.085

5.218 ± 0.12

3.486 ± 0.095

3.377 ± 0.093

4.577 ± 0.088

6.355 ± 0.112

5.712 ± 0.189

6.638 ± 0.14

1.483 ± 0.058

3.961 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski