filamentous cyanobacterium CCP5

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; unclassified Cyanobacteria

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5193 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P8WHX5|A0A2P8WHX5_9CYAN S-(hydroxymethyl)glutathione dehydrogenase OS=filamentous cyanobacterium CCP5 OX=2107701 GN=C7271_07370 PE=3 SV=1
MM1 pKa = 7.46SPVILISACLLAGGCQPQSEE21 pKa = 4.76PNVDD25 pKa = 3.38APLSGEE31 pKa = 3.99QVTILGTLTGVAEE44 pKa = 4.6EE45 pKa = 4.26KK46 pKa = 11.02LEE48 pKa = 4.16AALEE52 pKa = 4.16PFTAKK57 pKa = 9.87TGIEE61 pKa = 4.12VVYY64 pKa = 10.22EE65 pKa = 4.05GTDD68 pKa = 2.95AFTTLIPVRR77 pKa = 11.84VDD79 pKa = 3.37SNNTPDD85 pKa = 3.55IALFPQPGLMADD97 pKa = 4.78FAAEE101 pKa = 4.09GQMVPLDD108 pKa = 3.79SFMDD112 pKa = 3.75MDD114 pKa = 4.24QLAAAYY120 pKa = 10.05DD121 pKa = 4.25DD122 pKa = 3.9YY123 pKa = 11.22WLEE126 pKa = 4.02LTSLDD131 pKa = 3.44GHH133 pKa = 7.02PYY135 pKa = 9.85GVWIRR140 pKa = 11.84ADD142 pKa = 3.47VKK144 pKa = 11.18SLVWYY149 pKa = 10.36NPAAFAAAGYY159 pKa = 8.49GVPTDD164 pKa = 3.69WQGLEE169 pKa = 3.96ALMDD173 pKa = 3.85QIVADD178 pKa = 5.54GGTPWCLGMEE188 pKa = 4.34SGKK191 pKa = 9.2ATGWVGTDD199 pKa = 2.85WVEE202 pKa = 3.88EE203 pKa = 3.99LLLRR207 pKa = 11.84MAGPEE212 pKa = 4.02VYY214 pKa = 10.27DD215 pKa = 2.92RR216 pKa = 11.84WVAHH220 pKa = 7.02EE221 pKa = 4.51IPFNAPEE228 pKa = 3.88VKK230 pKa = 10.32AAFEE234 pKa = 4.02QFGAIARR241 pKa = 11.84SPDD244 pKa = 3.65YY245 pKa = 10.97VYY247 pKa = 11.26GGATSAISTPFGDD260 pKa = 4.03APRR263 pKa = 11.84PLFDD267 pKa = 4.74QPPGCYY273 pKa = 9.03LHH275 pKa = 6.86RR276 pKa = 11.84QASFIEE282 pKa = 4.35EE283 pKa = 4.43FFPASAVPAEE293 pKa = 4.39TVSLFPLPAMDD304 pKa = 4.86EE305 pKa = 4.46GPAPVLVSGIVFGLFNDD322 pKa = 4.39TPAARR327 pKa = 11.84ALMEE331 pKa = 4.1YY332 pKa = 10.4LATPEE337 pKa = 4.15PHH339 pKa = 6.93EE340 pKa = 4.06IWAGLGSYY348 pKa = 10.18ISPHH352 pKa = 5.7RR353 pKa = 11.84QVGLDD358 pKa = 3.85AYY360 pKa = 10.02PDD362 pKa = 3.7VLTQRR367 pKa = 11.84QAEE370 pKa = 4.12ILQNAEE376 pKa = 3.87VVRR379 pKa = 11.84FDD381 pKa = 5.41GSDD384 pKa = 3.47LMPGAVGTGSFWSGVVDD401 pKa = 3.6YY402 pKa = 11.61VGGEE406 pKa = 4.25DD407 pKa = 4.68LDD409 pKa = 3.89QVLADD414 pKa = 5.38IEE416 pKa = 4.67ASWPTEE422 pKa = 3.8EE423 pKa = 4.69

Molecular weight: 45.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P8WCE6|A0A2P8WCE6_9CYAN DNA-binding response regulator OS=filamentous cyanobacterium CCP5 OX=2107701 GN=C7271_17785 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 9.06RR4 pKa = 11.84TLGGTNRR11 pKa = 11.84KK12 pKa = 7.61QKK14 pKa = 8.99RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 7.41TGQQVIKK33 pKa = 9.99SRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.22GRR40 pKa = 11.84ARR42 pKa = 11.84LAVV45 pKa = 3.42

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5193

0

5193

1436203

27

2385

276.6

30.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.869 ± 0.04

0.935 ± 0.011

5.472 ± 0.029

5.869 ± 0.035

3.661 ± 0.024

7.479 ± 0.037

2.01 ± 0.021

5.549 ± 0.025

3.014 ± 0.025

11.256 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.022 ± 0.017

3.09 ± 0.023

5.354 ± 0.028

5.473 ± 0.036

6.056 ± 0.029

6.16 ± 0.029

5.417 ± 0.025

6.943 ± 0.029

1.581 ± 0.018

2.792 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski