Lupinus albus (White lupine) (Lupinus termis)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; genistoids sensu lato;

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46596 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A5MN57|A0A6A5MN57_LUPAL Putative Snf7 family protein OS=Lupinus albus OX=3870 GN=Lal_00006476 PE=4 SV=1
MM1 pKa = 7.84LIHH4 pKa = 6.89SFNSQDD10 pKa = 3.31GAKK13 pKa = 10.11INIDD17 pKa = 3.05GTGYY21 pKa = 10.49LNGYY25 pKa = 9.03HH26 pKa = 6.85GEE28 pKa = 4.06TSATFFCGDD37 pKa = 4.32DD38 pKa = 3.52DD39 pKa = 5.25DD40 pKa = 4.47EE41 pKa = 4.98ARR43 pKa = 11.84KK44 pKa = 9.25LVQDD48 pKa = 3.97LTVDD52 pKa = 3.8DD53 pKa = 5.3LLTNVDD59 pKa = 4.31LSSINYY65 pKa = 7.8VIWNDD70 pKa = 2.55NWTAVAEE77 pKa = 4.46DD78 pKa = 4.56GGLSAQFEE86 pKa = 4.61HH87 pKa = 7.3TIMIIPDD94 pKa = 3.68GVEE97 pKa = 4.34TITQCC102 pKa = 4.79

Molecular weight: 11.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A5NZQ6|A0A6A5NZQ6_LUPAL Uncharacterized protein OS=Lupinus albus OX=3870 GN=Lal_00041115 PE=3 SV=1
MM1 pKa = 7.52PRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84SIFIRR10 pKa = 11.84ITRR13 pKa = 11.84QPPQSTPIQPHH24 pKa = 6.87HH25 pKa = 6.27IQVPIRR31 pKa = 11.84LKK33 pKa = 10.98LVGQRR38 pKa = 11.84HH39 pKa = 5.27RR40 pKa = 11.84LRR42 pKa = 11.84RR43 pKa = 11.84HH44 pKa = 4.13TTQVRR49 pKa = 11.84RR50 pKa = 11.84EE51 pKa = 3.94QQPLSVATKK60 pKa = 9.32TNMVQPLPLCLHH72 pKa = 6.51SLRR75 pKa = 11.84HH76 pKa = 5.88LNPPQPIPIRR86 pKa = 11.84PHH88 pKa = 5.71HH89 pKa = 6.99VNTKK93 pKa = 9.15PFTIFWIIPII103 pKa = 4.26

Molecular weight: 12.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46596

0

46596

18656812

51

7189

400.4

44.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.831 ± 0.017

1.698 ± 0.006

5.363 ± 0.008

6.284 ± 0.012

4.141 ± 0.008

6.656 ± 0.016

2.652 ± 0.007

5.584 ± 0.012

5.985 ± 0.014

9.317 ± 0.012

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.446 ± 0.006

4.702 ± 0.01

4.918 ± 0.014

3.831 ± 0.008

5.568 ± 0.022

8.432 ± 0.017

5.036 ± 0.008

6.484 ± 0.009

1.208 ± 0.004

2.861 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski