Flavobacterium phage FLiP

Taxonomy: Viruses; Finnlakeviridae; Finnlakevirus; Flavobacterium virus FLiP

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222NP85|A0A222NP85_9VIRU Uncharacterized protein OS=Flavobacterium phage FLiP OX=2023716 PE=4 SV=1
MM1 pKa = 7.5SVLAFQKK8 pKa = 10.8LSGDD12 pKa = 3.55LSTDD16 pKa = 2.96GKK18 pKa = 10.81ILVEE22 pKa = 3.95LQKK25 pKa = 11.09LNQKK29 pKa = 10.25NGLPDD34 pKa = 3.8LYY36 pKa = 11.37YY37 pKa = 10.82FFDD40 pKa = 3.16KK41 pKa = 10.73LYY43 pKa = 10.11PNRR46 pKa = 11.84EE47 pKa = 3.66PFLIEE52 pKa = 3.65RR53 pKa = 11.84SFNSGEE59 pKa = 3.75PDD61 pKa = 2.9GWFPRR66 pKa = 11.84YY67 pKa = 8.77WNNDD71 pKa = 3.45PNDD74 pKa = 3.66EE75 pKa = 4.37KK76 pKa = 11.18PFGGSFWGYY85 pKa = 8.77WNLTAFSGGEE95 pKa = 3.81NEE97 pKa = 5.11DD98 pKa = 3.66RR99 pKa = 11.84DD100 pKa = 4.23DD101 pKa = 4.26ASLVNSDD108 pKa = 3.94GQLVCYY114 pKa = 10.13VSNRR118 pKa = 11.84EE119 pKa = 3.82SHH121 pKa = 5.86ASYY124 pKa = 9.74MGEE127 pKa = 3.67ATKK130 pKa = 9.99MKK132 pKa = 10.41RR133 pKa = 11.84HH134 pKa = 5.79GGYY137 pKa = 10.06INVNAHH143 pKa = 6.02FLGFAVIDD151 pKa = 3.37QFAYY155 pKa = 8.09NTKK158 pKa = 9.93GFNGNVQIEE167 pKa = 4.39YY168 pKa = 10.71DD169 pKa = 3.85EE170 pKa = 5.08SPLEE174 pKa = 4.86KK175 pKa = 10.69GDD177 pKa = 3.46ISNGKK182 pKa = 10.1SYY184 pKa = 11.52VDD186 pKa = 3.26WFEE189 pKa = 5.96DD190 pKa = 2.88SDD192 pKa = 4.68LVVKK196 pKa = 10.55SPYY199 pKa = 8.93FFKK202 pKa = 11.05VSGEE206 pKa = 4.29GEE208 pKa = 3.98PPRR211 pKa = 11.84YY212 pKa = 8.71PEE214 pKa = 4.38KK215 pKa = 11.2VGDD218 pKa = 4.41LYY220 pKa = 11.29QFSVTADD227 pKa = 2.57IGQYY231 pKa = 10.37YY232 pKa = 9.96LIEE235 pKa = 4.43VADD238 pKa = 4.52FPYY241 pKa = 10.63FDD243 pKa = 4.54ILVDD247 pKa = 4.16FPFNAEE253 pKa = 3.82VQYY256 pKa = 11.09SYY258 pKa = 11.2AYY260 pKa = 8.58EE261 pKa = 4.06PPFIDD266 pKa = 5.37FNTVQNKK273 pKa = 8.93KK274 pKa = 10.55ASVQITQLTGFKK286 pKa = 10.25VRR288 pKa = 11.84SLTRR292 pKa = 11.84FDD294 pKa = 5.44AVDD297 pKa = 3.63SNDD300 pKa = 3.32NNTYY304 pKa = 10.1SAFVEE309 pKa = 4.35STGFYY314 pKa = 10.61EE315 pKa = 4.62MNFGVQAHH323 pKa = 6.88LDD325 pKa = 3.28KK326 pKa = 11.48SFILIRR332 pKa = 11.84NHH334 pKa = 7.17AEE336 pKa = 3.53

Molecular weight: 38.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222NP96|A0A222NP96_9VIRU Uncharacterized protein OS=Flavobacterium phage FLiP OX=2023716 PE=4 SV=1
MM1 pKa = 7.29AQRR4 pKa = 11.84PRR6 pKa = 11.84SKK8 pKa = 10.46RR9 pKa = 11.84FTGFRR14 pKa = 11.84RR15 pKa = 11.84FRR17 pKa = 11.84PSFGRR22 pKa = 11.84MRR24 pKa = 11.84RR25 pKa = 11.84TYY27 pKa = 10.24SNYY30 pKa = 7.99RR31 pKa = 11.84QRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84TRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84TTTNRR44 pKa = 11.84RR45 pKa = 11.84NNMSKK50 pKa = 10.19YY51 pKa = 10.21IKK53 pKa = 9.97FAVIGVIIFLIIKK66 pKa = 9.96NKK68 pKa = 9.19TKK70 pKa = 10.64VLEE73 pKa = 4.28FFKK76 pKa = 11.09KK77 pKa = 9.55LTGKK81 pKa = 8.23KK82 pKa = 7.1TAA84 pKa = 4.13

Molecular weight: 10.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

2623

69

447

163.9

18.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.376 ± 0.628

0.801 ± 0.172

6.291 ± 0.514

5.719 ± 0.502

6.557 ± 0.598

4.804 ± 0.733

1.677 ± 0.434

6.634 ± 0.487

8.578 ± 0.61

8.73 ± 0.542

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.44 ± 0.21

6.214 ± 0.39

3.05 ± 0.301

3.584 ± 0.377

4.003 ± 0.821

6.977 ± 0.574

5.566 ± 0.422

7.053 ± 0.563

0.991 ± 0.191

4.956 ± 0.534
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski