Escherichia phage EcSzw_1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ounavirinae; Suspvirus; unclassified Suspvirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 130 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411BGX5|A0A411BGX5_9CAUD Uncharacterized protein OS=Escherichia phage EcSzw_1 OX=2419742 GN=EcSzw1_3 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 9.8CFHH5 pKa = 6.61GTTQEE10 pKa = 3.94NFVNLINGGDD20 pKa = 3.8KK21 pKa = 10.83PSGAWNCSDD30 pKa = 4.52SDD32 pKa = 3.86GCFYY36 pKa = 11.09VYY38 pKa = 10.13PVNKK42 pKa = 10.0FYY44 pKa = 11.24DD45 pKa = 4.3LDD47 pKa = 3.88SMNAEE52 pKa = 4.39EE53 pKa = 4.51ITNEE57 pKa = 3.97GLRR60 pKa = 11.84NALDD64 pKa = 3.6SATITAAFQMKK75 pKa = 7.87TQNIVILEE83 pKa = 4.05LDD85 pKa = 3.19IPEE88 pKa = 5.18YY89 pKa = 10.5EE90 pKa = 4.74LQDD93 pKa = 3.54DD94 pKa = 4.64WSCEE98 pKa = 3.87NMSDD102 pKa = 2.97IASFTEE108 pKa = 4.54SFDD111 pKa = 3.62KK112 pKa = 11.01DD113 pKa = 3.61WIKK116 pKa = 10.82KK117 pKa = 9.6VYY119 pKa = 8.44ITEE122 pKa = 4.2FNSMYY127 pKa = 10.9SPFFVPNLKK136 pKa = 10.21NPNLGYY142 pKa = 10.38VDD144 pKa = 4.01EE145 pKa = 4.92KK146 pKa = 11.24LEE148 pKa = 4.09EE149 pKa = 4.11VASFIQNTDD158 pKa = 3.18DD159 pKa = 5.45SSIIYY164 pKa = 10.47CNMLDD169 pKa = 4.55AMQEE173 pKa = 4.18SLTEE177 pKa = 4.06STVNDD182 pKa = 3.45VLAA185 pKa = 4.9

Molecular weight: 21.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411BH11|A0A411BH11_9CAUD Ribonucleoside-diphosphate reductase OS=Escherichia phage EcSzw_1 OX=2419742 GN=EcSzw1_45 PE=3 SV=1
MM1 pKa = 7.71GIFSGMKK8 pKa = 9.73SRR10 pKa = 11.84MLAALAKK17 pKa = 8.7VAAVIATTGKK27 pKa = 10.39DD28 pKa = 2.93AGIDD32 pKa = 3.17IKK34 pKa = 10.34TVTDD38 pKa = 4.37SINAQTLAQPYY49 pKa = 9.33IYY51 pKa = 10.27VGRR54 pKa = 11.84GKK56 pKa = 10.59GGKK59 pKa = 8.68KK60 pKa = 8.45QAHH63 pKa = 5.92RR64 pKa = 11.84QTGAAAIKK72 pKa = 10.1RR73 pKa = 11.84AAKK76 pKa = 9.87KK77 pKa = 10.13ARR79 pKa = 11.84NRR81 pKa = 11.84KK82 pKa = 8.79RR83 pKa = 11.84NKK85 pKa = 9.84

Molecular weight: 9.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

130

0

130

25585

29

899

196.8

22.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.379 ± 0.279

1.305 ± 0.106

6.301 ± 0.186

6.695 ± 0.272

4.284 ± 0.149

6.586 ± 0.22

2.052 ± 0.138

5.867 ± 0.161

7.727 ± 0.275

7.915 ± 0.161

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.92 ± 0.13

5.089 ± 0.191

2.951 ± 0.116

3.58 ± 0.155

4.174 ± 0.135

6.261 ± 0.21

6.328 ± 0.319

7.008 ± 0.184

1.223 ± 0.095

4.331 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski