Stenotrophomonas phage Smp131

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Simpcentumvirus; Stenotrophomonas virus Smp131

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9IQH2|V9IQH2_9CAUD Tail protein OS=Stenotrophomonas phage Smp131 OX=1168563 GN=E' PE=4 SV=1
MM1 pKa = 7.3SCPYY5 pKa = 10.86GEE7 pKa = 4.42AHH9 pKa = 6.61TDD11 pKa = 3.58YY12 pKa = 11.25VPWLASAAGSLAAWAPPSVGEE33 pKa = 4.36QVTLVCSDD41 pKa = 3.6GDD43 pKa = 3.84LANAVALRR51 pKa = 11.84GLYY54 pKa = 9.88CDD56 pKa = 4.33QYY58 pKa = 11.02PAPSDD63 pKa = 3.55NADD66 pKa = 3.11LVMVKK71 pKa = 10.41FSDD74 pKa = 4.16GAVISYY80 pKa = 10.76DD81 pKa = 3.81SASHH85 pKa = 6.55ALAAILPSGGIATITADD102 pKa = 3.61GGVTINGPVTINGDD116 pKa = 3.57TSITGQVTINGKK128 pKa = 9.96AEE130 pKa = 3.79ASEE133 pKa = 4.34DD134 pKa = 3.8VVGGGISLKK143 pKa = 7.05QHH145 pKa = 5.25KK146 pKa = 9.58HH147 pKa = 4.91GAVQPGSGTSGPPAA161 pKa = 4.58

Molecular weight: 15.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9IQK9|V9IQK9_9CAUD Major tail sheath protein OS=Stenotrophomonas phage Smp131 OX=1168563 GN=FI PE=3 SV=1
MM1 pKa = 7.29TEE3 pKa = 3.77VRR5 pKa = 11.84TLLPPNATTLEE16 pKa = 4.19RR17 pKa = 11.84AVEE20 pKa = 4.18GADD23 pKa = 3.45AQLMGIPMVHH33 pKa = 6.51HH34 pKa = 6.75LLWNPWTCPAEE45 pKa = 4.09FLPFLAWTVSVDD57 pKa = 3.0TWDD60 pKa = 4.11SEE62 pKa = 3.93WPEE65 pKa = 3.79QIKK68 pKa = 10.1RR69 pKa = 11.84ARR71 pKa = 11.84IASSFQIQRR80 pKa = 11.84HH81 pKa = 5.69KK82 pKa = 10.79GTAQSIADD90 pKa = 3.64VVASFGGQVQLRR102 pKa = 11.84EE103 pKa = 4.32WWQMDD108 pKa = 4.08PPGQPHH114 pKa = 5.71TFEE117 pKa = 5.32LLLTLSGQGGQDD129 pKa = 3.01ATADD133 pKa = 4.35FIDD136 pKa = 3.68QVMAAVDD143 pKa = 3.36RR144 pKa = 11.84AKK146 pKa = 10.25PVRR149 pKa = 11.84SHH151 pKa = 6.64YY152 pKa = 11.33SFTQGINAEE161 pKa = 4.17GAIGVLGVARR171 pKa = 11.84VLTSTRR177 pKa = 11.84LQLAAANRR185 pKa = 11.84RR186 pKa = 11.84TYY188 pKa = 9.9PCPSPRR194 pKa = 11.84SPLRR198 pKa = 11.84RR199 pKa = 11.84QALPPSSTQRR209 pKa = 11.84TRR211 pKa = 11.84VPRR214 pKa = 11.84RR215 pKa = 11.84SRR217 pKa = 11.84SHH219 pKa = 6.84RR220 pKa = 11.84SAA222 pKa = 3.9

Molecular weight: 24.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

10057

38

952

218.6

23.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.539 ± 0.597

1.114 ± 0.132

5.35 ± 0.265

4.922 ± 0.307

3.082 ± 0.262

8.303 ± 0.504

2.277 ± 0.205

4.067 ± 0.216

3.192 ± 0.3

9.168 ± 0.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.297 ± 0.158

2.675 ± 0.22

5.439 ± 0.404

4.564 ± 0.323

8.213 ± 0.463

6.006 ± 0.37

6.473 ± 0.302

6.543 ± 0.295

1.482 ± 0.167

2.297 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski