Mycobacterium phage Spikelee

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385DU50|A0A385DU50_9CAUD Uncharacterized protein OS=Mycobacterium phage Spikelee OX=2301571 GN=53 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 6.3SIDD5 pKa = 4.49TDD7 pKa = 3.71EE8 pKa = 4.56QLNGYY13 pKa = 8.47PPNTVILDD21 pKa = 3.65ADD23 pKa = 3.36EE24 pKa = 4.41WAWQKK29 pKa = 11.11DD30 pKa = 4.24VNQGSHH36 pKa = 5.75LWIPAAFYY44 pKa = 11.28SEE46 pKa = 5.12LLVDD50 pKa = 4.39DD51 pKa = 5.19GGRR54 pKa = 11.84PSLPARR60 pKa = 11.84LLYY63 pKa = 10.51VPHH66 pKa = 7.11ANSANEE72 pKa = 4.01SS73 pKa = 3.51

Molecular weight: 8.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385DRS3|A0A385DRS3_9CAUD Tail assembly chaperone OS=Mycobacterium phage Spikelee OX=2301571 GN=14 PE=4 SV=1
MM1 pKa = 7.41SAFPPPRR8 pKa = 11.84TLTEE12 pKa = 5.0RR13 pKa = 11.84IQGAHH18 pKa = 6.7LNLKK22 pKa = 9.84LARR25 pKa = 11.84QSGNPDD31 pKa = 3.26IIAAAEE37 pKa = 4.34RR38 pKa = 11.84ILNQLVDD45 pKa = 3.97RR46 pKa = 11.84LPRR49 pKa = 11.84PTHH52 pKa = 5.73QEE54 pKa = 3.46

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

18469

30

1170

177.6

19.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.84 ± 0.509

1.072 ± 0.13

6.508 ± 0.23

5.853 ± 0.268

2.918 ± 0.169

8.885 ± 0.517

2.128 ± 0.177

4.548 ± 0.168

3.433 ± 0.177

7.499 ± 0.19

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.096

3.476 ± 0.17

5.972 ± 0.197

3.568 ± 0.188

6.676 ± 0.336

6.021 ± 0.284

6.351 ± 0.265

7.158 ± 0.278

2.29 ± 0.142

2.539 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski