Rubellimicrobium rubrum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Rubellimicrobium

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4087 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C4N509|A0A5C4N509_9RHOB DUF2852 domain-containing protein OS=Rubellimicrobium rubrum OX=2585369 GN=FHG66_00300 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 10.19RR4 pKa = 11.84SLSTLAAASLLLAVPAWAQVAAIAATDD31 pKa = 3.85LNLRR35 pKa = 11.84SGPGPEE41 pKa = 3.84QEE43 pKa = 4.08IVGLIPANEE52 pKa = 3.92AVSVEE57 pKa = 3.98GCLDD61 pKa = 3.87DD62 pKa = 6.61DD63 pKa = 3.68SWCRR67 pKa = 11.84VDD69 pKa = 4.14YY70 pKa = 11.21AGTLGWASGEE80 pKa = 4.15YY81 pKa = 9.15LTATLADD88 pKa = 3.75QPDD91 pKa = 4.39PVPLYY96 pKa = 10.48EE97 pKa = 5.26NRR99 pKa = 11.84TTLQVGSVTFDD110 pKa = 3.01QGQDD114 pKa = 3.0AATGAGALGGAALAAALVGGPAALVAGAAIGAATGAAVAPDD155 pKa = 4.51DD156 pKa = 3.85ATVTYY161 pKa = 10.32IRR163 pKa = 11.84EE164 pKa = 4.03NPADD168 pKa = 3.61PVYY171 pKa = 10.78LQGEE175 pKa = 4.73VIVGAGIPEE184 pKa = 4.14EE185 pKa = 4.62VEE187 pKa = 3.67ILPVPEE193 pKa = 4.17TNFGYY198 pKa = 10.58LYY200 pKa = 10.48VNGVPVVVDD209 pKa = 3.2QSNRR213 pKa = 11.84QIVYY217 pKa = 9.91IVRR220 pKa = 3.6

Molecular weight: 22.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C4MLH7|A0A5C4MLH7_9RHOB Amino acid ABC transporter substrate-binding protein OS=Rubellimicrobium rubrum OX=2585369 GN=FHG66_18485 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 9.2ARR15 pKa = 11.84HH16 pKa = 4.71GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84AVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.41VLCAA44 pKa = 3.88

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4087

0

4087

1295680

22

2465

317.0

34.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.803 ± 0.052

0.78 ± 0.012

5.89 ± 0.032

6.061 ± 0.036

3.386 ± 0.022

9.125 ± 0.04

2.056 ± 0.018

4.43 ± 0.029

2.149 ± 0.024

10.656 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.437 ± 0.017

2.187 ± 0.02

5.537 ± 0.032

3.217 ± 0.023

7.81 ± 0.044

5.056 ± 0.025

5.336 ± 0.025

7.635 ± 0.032

1.513 ± 0.017

1.936 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski