Methylomonas methanica (strain MC09)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methylomonas; Methylomonas methanica

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4435 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9ZYA3|F9ZYA3_METMM Dihydropteroate synthase OS=Methylomonas methanica (strain MC09) OX=857087 GN=Metme_2622 PE=3 SV=1
MM1 pKa = 7.23NLKK4 pKa = 8.61NTLVTSCLALACISSSANAASVLGISWDD32 pKa = 3.75PDD34 pKa = 3.47SLIDD38 pKa = 3.65FTASATQWEE47 pKa = 4.67SQPLGVIGEE56 pKa = 4.4TLTGYY61 pKa = 10.57GAFTSFQGQTIAQYY75 pKa = 11.32GGTNEE80 pKa = 3.9LTFVFTATLAAVDD93 pKa = 4.3LTNPTKK99 pKa = 10.36PVLTYY104 pKa = 10.41TPGHH108 pKa = 5.59VDD110 pKa = 2.91VWVTPVATYY119 pKa = 11.0APLALTAADD128 pKa = 4.49LASQTLANATGTAASLFLSLDD149 pKa = 3.51FNGDD153 pKa = 3.39LTGSATNLFTSAQNGTGVGALDD175 pKa = 3.84AVGGAALANFDD186 pKa = 4.6TNTIDD191 pKa = 4.26LGGGNFADD199 pKa = 4.72LNFSSSFNIAPGTPGTNSPIGYY221 pKa = 8.16PITGTLLVTGNTVNVPEE238 pKa = 4.68PATLTLLGLGMLGFGFTRR256 pKa = 11.84NKK258 pKa = 9.97KK259 pKa = 9.66AA260 pKa = 3.37

Molecular weight: 26.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0A180|G0A180_METMM Putative plasmid maintenance system antidote protein XRE family OS=Methylomonas methanica (strain MC09) OX=857087 GN=Metme_4148 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.28QPSKK9 pKa = 10.01LKK11 pKa = 10.39RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84RR29 pKa = 11.84VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.12GRR39 pKa = 11.84ASLTVV44 pKa = 3.04

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4435

0

4435

1455403

30

3471

328.2

36.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.61 ± 0.036

1.067 ± 0.015

5.629 ± 0.026

5.745 ± 0.038

4.098 ± 0.026

6.985 ± 0.04

2.313 ± 0.02

5.965 ± 0.033

4.637 ± 0.035

11.05 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.02

3.965 ± 0.03

4.4 ± 0.026

4.787 ± 0.03

5.46 ± 0.028

6.051 ± 0.029

5.031 ± 0.035

6.582 ± 0.034

1.338 ± 0.016

2.965 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski