Ralstonia phage RSB3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Jiaoyazivirus; Ralstonia virus RSB3

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3TM15|U3TM15_9CAUD Uncharacterized protein OS=Ralstonia phage RSB3 OX=1402875 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 9.79WIAGTNQPGYY12 pKa = 9.93MPDD15 pKa = 3.54DD16 pKa = 3.65MPAEE20 pKa = 4.3FEE22 pKa = 4.42SQDD25 pKa = 3.2EE26 pKa = 4.28AKK28 pKa = 10.29RR29 pKa = 11.84YY30 pKa = 9.98IIGQMKK36 pKa = 10.12YY37 pKa = 10.36DD38 pKa = 3.87EE39 pKa = 4.68EE40 pKa = 4.29NAASEE45 pKa = 4.32EE46 pKa = 4.4VAEE49 pKa = 4.56HH50 pKa = 6.25LASLAEE56 pKa = 4.18DD57 pKa = 3.91ANLEE61 pKa = 4.2SGEE64 pKa = 3.89FSYY67 pKa = 11.13LFNHH71 pKa = 6.0RR72 pKa = 11.84VYY74 pKa = 10.41WVTQDD79 pKa = 2.98TT80 pKa = 4.01

Molecular weight: 9.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3TJY1|U3TJY1_9CAUD Uncharacterized protein OS=Ralstonia phage RSB3 OX=1402875 PE=4 SV=1
MM1 pKa = 8.05ISGLYY6 pKa = 8.87FNAMPEE12 pKa = 4.16HH13 pKa = 6.54KK14 pKa = 9.93KK15 pKa = 10.04PRR17 pKa = 11.84RR18 pKa = 11.84AQSEE22 pKa = 3.88ASRR25 pKa = 11.84NRR27 pKa = 11.84GIKK30 pKa = 10.26GSMRR34 pKa = 11.84DD35 pKa = 3.38TSGVYY40 pKa = 10.31AKK42 pKa = 10.75AEE44 pKa = 3.92EE45 pKa = 3.92AAHH48 pKa = 4.85YY49 pKa = 7.89FKK51 pKa = 11.02RR52 pKa = 11.84KK53 pKa = 9.06VLPKK57 pKa = 9.99IEE59 pKa = 4.28AARR62 pKa = 11.84KK63 pKa = 7.99RR64 pKa = 11.84GEE66 pKa = 3.77VVRR69 pKa = 11.84FVNGRR74 pKa = 11.84WFINGKK80 pKa = 7.46QLEE83 pKa = 4.29AA84 pKa = 5.24

Molecular weight: 9.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

13284

54

1315

265.7

29.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.811 ± 0.581

0.964 ± 0.154

5.819 ± 0.2

5.842 ± 0.302

3.199 ± 0.185

7.641 ± 0.276

2.1 ± 0.191

4.306 ± 0.178

5.187 ± 0.293

8.22 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.943 ± 0.168

4.17 ± 0.236

4.216 ± 0.255

4.562 ± 0.262

5.638 ± 0.275

5.736 ± 0.381

6.067 ± 0.235

6.805 ± 0.246

1.483 ± 0.127

3.29 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski