Enterococcus phage EFC-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A097BY35|A0A097BY35_9CAUD Methyltransferase OS=Enterococcus phage EFC-1 OX=1486428 PE=4 SV=1
MM1 pKa = 7.66YY2 pKa = 10.78YY3 pKa = 9.81PDD5 pKa = 3.57EE6 pKa = 4.95DD7 pKa = 5.3KK8 pKa = 11.39NVEE11 pKa = 3.81WTIDD15 pKa = 3.62DD16 pKa = 3.67DD17 pKa = 4.35TGFIAPLINLEE28 pKa = 3.85NGMWGMIDD36 pKa = 4.61KK37 pKa = 10.34YY38 pKa = 11.64VLMQSTGFKK47 pKa = 10.25DD48 pKa = 3.65DD49 pKa = 3.71NGVEE53 pKa = 4.03IFEE56 pKa = 4.33GDD58 pKa = 3.6VVNIHH63 pKa = 6.4WFYY66 pKa = 11.04IDD68 pKa = 4.39YY69 pKa = 10.99DD70 pKa = 4.21PEE72 pKa = 4.04TLGAIEE78 pKa = 4.59NEE80 pKa = 3.75ATAEE84 pKa = 4.05KK85 pKa = 10.83VVITKK90 pKa = 9.97EE91 pKa = 3.88FGNLGFWWNSGDD103 pKa = 4.03DD104 pKa = 3.88WVDD107 pKa = 3.33LATLALTVQLHH118 pKa = 5.43EE119 pKa = 4.72EE120 pKa = 4.11SFEE123 pKa = 4.09LLGNVHH129 pKa = 6.74EE130 pKa = 4.6NPEE133 pKa = 4.24LLEE136 pKa = 4.38KK137 pKa = 10.76ASEE140 pKa = 4.14EE141 pKa = 4.21DD142 pKa = 3.57DD143 pKa = 4.65SEE145 pKa = 4.44VV146 pKa = 3.26

Molecular weight: 16.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A097BYA1|A0A097BYA1_9CAUD Tapemeasure domain-containing protein OS=Enterococcus phage EFC-1 OX=1486428 PE=4 SV=1
MM1 pKa = 7.63ARR3 pKa = 11.84NQVRR7 pKa = 11.84INGLAGISKK16 pKa = 10.06KK17 pKa = 10.56LKK19 pKa = 10.57RR20 pKa = 11.84NAQLDD25 pKa = 3.93DD26 pKa = 3.58VKK28 pKa = 11.14KK29 pKa = 10.31VVRR32 pKa = 11.84NNTAEE37 pKa = 4.08LTANMQAEE45 pKa = 4.34AGKK48 pKa = 10.5VLTGHH53 pKa = 6.39WEE55 pKa = 3.96GKK57 pKa = 10.35KK58 pKa = 9.82FVKK61 pKa = 8.91PTGATKK67 pKa = 10.42RR68 pKa = 11.84SIVMRR73 pKa = 11.84LSNNGFSGHH82 pKa = 6.26TGPGTEE88 pKa = 3.92YY89 pKa = 10.97APYY92 pKa = 9.6LIHH95 pKa = 6.37GTRR98 pKa = 11.84FMVKK102 pKa = 9.1RR103 pKa = 11.84DD104 pKa = 3.69FFLPPLKK111 pKa = 9.69QQKK114 pKa = 9.72VKK116 pKa = 10.51FRR118 pKa = 11.84TDD120 pKa = 3.28LEE122 pKa = 4.32RR123 pKa = 11.84LMKK126 pKa = 10.6

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

12628

25

1135

214.0

24.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.414 ± 0.424

0.665 ± 0.125

6.296 ± 0.228

8.077 ± 0.348

3.484 ± 0.203

5.836 ± 0.267

1.37 ± 0.2

6.889 ± 0.247

9.075 ± 0.4

8.315 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.732 ± 0.162

6.058 ± 0.251

3.08 ± 0.197

4.458 ± 0.319

3.69 ± 0.241

6.723 ± 0.318

5.86 ± 0.335

6.066 ± 0.202

1.212 ± 0.129

3.698 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski