Microbacterium lemovicicum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3276 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S9W656|A0A3S9W656_9MICO Uncharacterized protein OS=Microbacterium lemovicicum OX=1072463 GN=CVS47_00162 PE=4 SV=1
MM1 pKa = 6.93NTRR4 pKa = 11.84HH5 pKa = 5.38LTGTVALGAVAALALAGCASGNAAAGGSGDD35 pKa = 4.54AGDD38 pKa = 4.78KK39 pKa = 10.18GTITLGFLPSWTDD52 pKa = 3.64GLSTAYY58 pKa = 10.72LLEE61 pKa = 4.16NRR63 pKa = 11.84LEE65 pKa = 4.29GLGYY69 pKa = 8.06TVEE72 pKa = 4.2MQTLTEE78 pKa = 4.67AGPLYY83 pKa = 10.51TGLAQGDD90 pKa = 3.41IDD92 pKa = 4.7IYY94 pKa = 10.3PSAWPEE100 pKa = 3.99LTHH103 pKa = 6.41ASYY106 pKa = 9.96MEE108 pKa = 4.34KK109 pKa = 10.65YY110 pKa = 10.09GDD112 pKa = 4.02SIEE115 pKa = 5.29DD116 pKa = 3.19LGAYY120 pKa = 10.09YY121 pKa = 10.7DD122 pKa = 4.07DD123 pKa = 4.75AKK125 pKa = 10.67LTIAVPDD132 pKa = 3.81YY133 pKa = 11.63VDD135 pKa = 4.11IDD137 pKa = 5.14SIDD140 pKa = 3.63QLAGQADD147 pKa = 4.3RR148 pKa = 11.84FGGRR152 pKa = 11.84IIGIEE157 pKa = 3.98PGAGLTKK164 pKa = 9.39QTQEE168 pKa = 4.12SMLPGYY174 pKa = 10.41GLDD177 pKa = 3.79GEE179 pKa = 4.81YY180 pKa = 11.05EE181 pKa = 4.4LVTSSTAAMLTEE193 pKa = 4.92LDD195 pKa = 3.93NAITAQKK202 pKa = 10.4DD203 pKa = 3.18IAVTLWRR210 pKa = 11.84PFWANDD216 pKa = 3.3AYY218 pKa = 10.63DD219 pKa = 4.13VKK221 pKa = 11.22DD222 pKa = 3.61LADD225 pKa = 4.27PDD227 pKa = 3.69GRR229 pKa = 11.84MGEE232 pKa = 4.17PEE234 pKa = 4.06ALHH237 pKa = 5.69FLGRR241 pKa = 11.84EE242 pKa = 4.26GLGEE246 pKa = 4.46EE247 pKa = 4.53YY248 pKa = 10.26PDD250 pKa = 3.4IAEE253 pKa = 4.92LIAGIRR259 pKa = 11.84LDD261 pKa = 3.85DD262 pKa = 3.92EE263 pKa = 4.63QYY265 pKa = 11.42GALEE269 pKa = 4.09DD270 pKa = 4.39LVVNEE275 pKa = 4.11YY276 pKa = 11.42GEE278 pKa = 4.31GRR280 pKa = 11.84EE281 pKa = 4.24AEE283 pKa = 4.86AVQAWLEE290 pKa = 3.98QYY292 pKa = 10.8PDD294 pKa = 3.76VISS297 pKa = 3.61

Molecular weight: 31.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9W8S5|A0A3S9W8S5_9MICO ABC transporter glutamine-binding protein GlnH OS=Microbacterium lemovicicum OX=1072463 GN=glnH_3 PE=3 SV=1
MM1 pKa = 7.31VSGSRR6 pKa = 11.84TRR8 pKa = 11.84LVMASGSRR16 pKa = 11.84TRR18 pKa = 11.84LVMVSGSRR26 pKa = 11.84TRR28 pKa = 11.84LVMVSGSRR36 pKa = 11.84TRR38 pKa = 11.84LVMVSGSRR46 pKa = 11.84TRR48 pKa = 11.84LVMVSGSRR56 pKa = 11.84TRR58 pKa = 11.84LVMASGSRR66 pKa = 11.84TRR68 pKa = 11.84PVMVSASLMRR78 pKa = 11.84SATATAPMRR87 pKa = 11.84LVMASVVRR95 pKa = 11.84TRR97 pKa = 11.84RR98 pKa = 11.84AMVSGSRR105 pKa = 11.84TRR107 pKa = 11.84PVTVSGSRR115 pKa = 11.84TRR117 pKa = 11.84PVTVSGSRR125 pKa = 11.84TRR127 pKa = 11.84PVTVSGSRR135 pKa = 11.84TRR137 pKa = 11.84RR138 pKa = 11.84AMVSGSRR145 pKa = 11.84TLPVMVSGSRR155 pKa = 11.84TLPVMGTGSPTRR167 pKa = 11.84SVTATVRR174 pKa = 11.84TQLVMVSGSRR184 pKa = 11.84TRR186 pKa = 11.84PVMVSGSRR194 pKa = 11.84TRR196 pKa = 11.84PVMVSGSLTRR206 pKa = 11.84RR207 pKa = 11.84GMASVVRR214 pKa = 11.84TLPVMVSGTRR224 pKa = 11.84TRR226 pKa = 11.84PVMVSGSLTRR236 pKa = 11.84RR237 pKa = 11.84GMASVVRR244 pKa = 11.84TLPVMVSGSPTRR256 pKa = 11.84PATVSGTRR264 pKa = 11.84TRR266 pKa = 11.84PAMASVGRR274 pKa = 11.84TRR276 pKa = 11.84RR277 pKa = 11.84ARR279 pKa = 11.84GTVSPTRR286 pKa = 11.84SVTGTARR293 pKa = 11.84TQLAMASGIRR303 pKa = 11.84TRR305 pKa = 11.84RR306 pKa = 11.84GMASVVRR313 pKa = 11.84PLPVMVSGSRR323 pKa = 11.84TRR325 pKa = 11.84PVMVSGSPTLFVTVSGPRR343 pKa = 11.84TLPVTDD349 pKa = 3.48MPGRR353 pKa = 11.84TLPEE357 pKa = 3.85PVVNASTVRR366 pKa = 11.84RR367 pKa = 11.84TSFVRR372 pKa = 11.84CGLSTMTRR380 pKa = 11.84CSPTTSLPTPWTRR393 pKa = 11.84APGTSS398 pKa = 3.05

Molecular weight: 42.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3276

0

3276

1074964

29

2343

328.1

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.898 ± 0.057

0.463 ± 0.009

6.542 ± 0.04

5.417 ± 0.04

3.088 ± 0.024

9.066 ± 0.033

1.905 ± 0.02

4.424 ± 0.032

1.714 ± 0.031

9.943 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.763 ± 0.017

1.773 ± 0.024

5.56 ± 0.03

2.676 ± 0.024

7.483 ± 0.051

5.658 ± 0.032

6.077 ± 0.038

9.124 ± 0.041

1.497 ± 0.015

1.929 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski