Citrobacter rodentium (strain ICC168) (Citrobacter freundii biotype 4280)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Citrobacter; Citrobacter rodentium

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2TKZ4|D2TKZ4_CITRI Protein translocase subunit SecD OS=Citrobacter rodentium (strain ICC168) OX=637910 GN=secD PE=3 SV=1
MM1 pKa = 7.37HH2 pKa = 6.88SWKK5 pKa = 10.29KK6 pKa = 10.49KK7 pKa = 9.88LVVSQLALACTLAITSQANASTDD30 pKa = 3.2ISGTTYY36 pKa = 9.9NTFTHH41 pKa = 6.67YY42 pKa = 11.13DD43 pKa = 3.46NSTHH47 pKa = 7.01ADD49 pKa = 3.11GVYY52 pKa = 10.12FEE54 pKa = 6.37GYY56 pKa = 10.12VGWNNYY62 pKa = 7.06ATNSVYY68 pKa = 10.77NGNIYY73 pKa = 10.03PVIKK77 pKa = 10.31NSTVNGVISTYY88 pKa = 11.11YY89 pKa = 10.71LDD91 pKa = 6.04DD92 pKa = 4.39GLATNANDD100 pKa = 3.82NSLTIKK106 pKa = 10.63NSTIHH111 pKa = 6.74GMITSEE117 pKa = 5.7CMTTDD122 pKa = 3.31CVNRR126 pKa = 11.84DD127 pKa = 2.87TGYY130 pKa = 11.24VYY132 pKa = 10.89DD133 pKa = 4.7RR134 pKa = 11.84LALTVDD140 pKa = 3.46NSTIDD145 pKa = 3.71DD146 pKa = 3.99NYY148 pKa = 11.03EE149 pKa = 3.75HH150 pKa = 6.27YY151 pKa = 9.43TYY153 pKa = 11.16NGTYY157 pKa = 9.91TDD159 pKa = 3.89GTADD163 pKa = 3.15THH165 pKa = 8.07IFDD168 pKa = 4.11VYY170 pKa = 10.92NLGVAVTLDD179 pKa = 3.44QEE181 pKa = 4.04VDD183 pKa = 3.73LVIKK187 pKa = 10.62NNSHH191 pKa = 5.52VAGISLIQGYY201 pKa = 7.39EE202 pKa = 3.89WEE204 pKa = 4.53DD205 pKa = 3.38TDD207 pKa = 6.0DD208 pKa = 3.89NTVSTGVNSSKK219 pKa = 10.7VFTNKK224 pKa = 8.23ITVADD229 pKa = 3.85STVTSGSWSDD239 pKa = 3.49EE240 pKa = 3.9GTSGWFGNNNNASDD254 pKa = 4.27YY255 pKa = 11.02NGSGYY260 pKa = 10.66SADD263 pKa = 3.92DD264 pKa = 3.51VALEE268 pKa = 4.55VYY270 pKa = 10.79ANPFADD276 pKa = 3.64NAMQTTATFSNSTLMGDD293 pKa = 3.67VVFASNFDD301 pKa = 3.87EE302 pKa = 5.01NFFPNGADD310 pKa = 3.38SYY312 pKa = 11.52RR313 pKa = 11.84DD314 pKa = 3.0TDD316 pKa = 3.97TNVDD320 pKa = 3.5TNGWDD325 pKa = 3.46GTDD328 pKa = 4.42RR329 pKa = 11.84LDD331 pKa = 3.35LTLNNGSKK339 pKa = 9.61WVGAAMSVHH348 pKa = 6.01QVDD351 pKa = 3.42TDD353 pKa = 3.49GDD355 pKa = 4.02GVYY358 pKa = 10.9DD359 pKa = 4.09SIEE362 pKa = 4.25TGTEE366 pKa = 3.75ATSTLIDD373 pKa = 3.26IAANSLWPWSTYY385 pKa = 10.72GVDD388 pKa = 4.19NSDD391 pKa = 2.89TAYY394 pKa = 10.74DD395 pKa = 3.92EE396 pKa = 4.65NGHH399 pKa = 5.07VVGNEE404 pKa = 4.09VYY406 pKa = 10.48QSGLFNVTLNNGSQWDD422 pKa = 4.02TTKK425 pKa = 11.06SSLIDD430 pKa = 3.36TLNINSGSIVNVADD444 pKa = 3.38STLISDD450 pKa = 4.98TIDD453 pKa = 3.46LTGGAALNINEE464 pKa = 5.08DD465 pKa = 3.5GHH467 pKa = 6.13VATDD471 pKa = 3.41KK472 pKa = 10.69LTVNNSTVTIADD484 pKa = 3.86DD485 pKa = 4.15VSAGWGVNDD494 pKa = 3.83AALYY498 pKa = 10.94ANTINVTNNGVLDD511 pKa = 3.97VGNSTAYY518 pKa = 10.41ALQADD523 pKa = 4.43TLNLTSYY530 pKa = 10.28TDD532 pKa = 3.68ANGHH536 pKa = 4.91VNAGVFNVHH545 pKa = 6.3SNSFVLDD552 pKa = 3.52ADD554 pKa = 4.01LTNDD558 pKa = 3.82RR559 pKa = 11.84TWDD562 pKa = 3.65TTKK565 pKa = 11.01SNYY568 pKa = 10.07GYY570 pKa = 10.91GIIAMNSDD578 pKa = 3.0GHH580 pKa = 5.83LTINGNGDD588 pKa = 3.59AYY590 pKa = 11.11SADD593 pKa = 3.56LAEE596 pKa = 4.63VDD598 pKa = 3.6NAGDD602 pKa = 3.73GVAAATGNYY611 pKa = 8.97KK612 pKa = 10.02VRR614 pKa = 11.84IDD616 pKa = 3.45NATGEE621 pKa = 4.47GSVADD626 pKa = 3.94YY627 pKa = 10.58KK628 pKa = 11.15GKK630 pKa = 10.29EE631 pKa = 3.97LIYY634 pKa = 11.28VNDD637 pKa = 4.05KK638 pKa = 11.23NSTATFSAANKK649 pKa = 9.8ADD651 pKa = 3.85LGAFTYY657 pKa = 9.0QARR660 pKa = 11.84QEE662 pKa = 4.35GNTVVLQQMQLTDD675 pKa = 4.02YY676 pKa = 11.6ANMALSIPSANSNIWNLQQDD696 pKa = 4.22TVGTRR701 pKa = 11.84LTNARR706 pKa = 11.84HH707 pKa = 5.4GLADD711 pKa = 4.02NGGAWVSYY719 pKa = 10.43FGGNFNGDD727 pKa = 3.43NGTIKK732 pKa = 10.58YY733 pKa = 9.65DD734 pKa = 3.42QDD736 pKa = 3.57VNGIMVGVDD745 pKa = 2.97TKK747 pKa = 11.02IDD749 pKa = 3.49GNNAKK754 pKa = 9.7WIVGAAAGFAKK765 pKa = 10.6GDD767 pKa = 3.43MSDD770 pKa = 2.99RR771 pKa = 11.84TGQVDD776 pKa = 3.54QDD778 pKa = 3.84SQSAYY783 pKa = 8.65IYY785 pKa = 10.6SSARR789 pKa = 11.84FANDD793 pKa = 2.85VFVDD797 pKa = 4.13GNLSYY802 pKa = 11.64SHH804 pKa = 7.17FNNDD808 pKa = 2.42LTANMSNGQYY818 pKa = 10.78VDD820 pKa = 3.92GNTSADD826 pKa = 2.84AWGFGLKK833 pKa = 10.32LGYY836 pKa = 9.47DD837 pKa = 3.67WKK839 pKa = 11.2LGDD842 pKa = 4.4AGYY845 pKa = 7.83LTPYY849 pKa = 10.28GSVSGLFQSGDD860 pKa = 3.73DD861 pKa = 3.74YY862 pKa = 11.66RR863 pKa = 11.84LSNNLRR869 pKa = 11.84VDD871 pKa = 3.55GQSYY875 pKa = 11.16DD876 pKa = 3.53SIRR879 pKa = 11.84YY880 pKa = 8.18EE881 pKa = 4.02LGVDD885 pKa = 3.24AGYY888 pKa = 8.52TFTYY892 pKa = 10.73SDD894 pKa = 4.21DD895 pKa = 3.81QAMTPYY901 pKa = 10.4FKK903 pKa = 10.62LAYY906 pKa = 10.48VYY908 pKa = 11.28DD909 pKa = 4.28DD910 pKa = 4.6ANNDD914 pKa = 2.97AHH916 pKa = 7.41VNGDD920 pKa = 3.88SIDD923 pKa = 3.67NGTEE927 pKa = 3.72GSAVRR932 pKa = 11.84VGLGTQFSFTKK943 pKa = 10.49NFSAYY948 pKa = 9.52TDD950 pKa = 3.49VNYY953 pKa = 10.9LGGGDD958 pKa = 4.37VDD960 pKa = 4.38QDD962 pKa = 2.79WSANLGVKK970 pKa = 7.39YY971 pKa = 8.73TWW973 pKa = 3.31

Molecular weight: 104.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2THH0|D2THH0_CITRI VOC domain-containing protein OS=Citrobacter rodentium (strain ICC168) OX=637910 GN=ROD_14011 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4792

0

4792

1494618

14

4316

311.9

34.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.874 ± 0.046

1.116 ± 0.016

5.156 ± 0.033

5.67 ± 0.033

3.833 ± 0.027

7.491 ± 0.041

2.192 ± 0.018

5.812 ± 0.029

4.251 ± 0.033

10.701 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.02

3.822 ± 0.032

4.429 ± 0.026

4.375 ± 0.032

5.794 ± 0.042

5.963 ± 0.035

5.428 ± 0.045

7.027 ± 0.03

1.519 ± 0.018

2.825 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski