Leptospira licerasiae serovar Varillal str. VAR 010

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Leptospirales; Leptospiraceae; Leptospira; Leptospira licerasiae; Leptospira licerasiae serovar Varillal

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3927 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0XPK7|I0XPK7_9LEPT Uncharacterized protein OS=Leptospira licerasiae serovar Varillal str. VAR 010 OX=1049972 GN=LEP1GSC185_0180 PE=3 SV=1
MM1 pKa = 7.6LIIHH5 pKa = 6.22NVSCGLVLTGTDD17 pKa = 3.01VGKK20 pKa = 10.71EE21 pKa = 3.73FLGLSEE27 pKa = 4.75EE28 pKa = 4.31DD29 pKa = 3.49KK30 pKa = 11.13KK31 pKa = 11.35DD32 pKa = 3.6PEE34 pKa = 4.01KK35 pKa = 10.55QAAVILSVLGVVSSPYY51 pKa = 10.42LLQFNPPVLTLAEE64 pKa = 4.82SISGSYY70 pKa = 8.4TVSIKK75 pKa = 10.95APIPSEE81 pKa = 3.43WSGNGEE87 pKa = 4.16SVLQAGLNYY96 pKa = 9.81INCPPQSDD104 pKa = 4.76DD105 pKa = 3.63NPSSVVFSGSSNYY118 pKa = 9.85SAQQSFARR126 pKa = 11.84FLEE129 pKa = 4.53PGSCVIEE136 pKa = 4.67FFAASYY142 pKa = 10.8SSDD145 pKa = 2.99MDD147 pKa = 3.7FAEE150 pKa = 5.1GEE152 pKa = 4.51TIGILPVIVNPNAVITASVPDD173 pKa = 3.7

Molecular weight: 18.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0XL16|I0XL16_9LEPT 7TM diverse intracellular signaling domain / adenylate/guanylate cyclase catalytic domain multi-domain protein OS=Leptospira licerasiae serovar Varillal str. VAR 010 OX=1049972 GN=LEP1GSC185_0739 PE=4 SV=1
MM1 pKa = 7.68DD2 pKa = 5.57RR3 pKa = 11.84YY4 pKa = 9.73ATTHH8 pKa = 5.91SGFATFCIVTALAWQARR25 pKa = 11.84SNAKK29 pKa = 9.65RR30 pKa = 11.84RR31 pKa = 11.84NTLVVRR37 pKa = 11.84RR38 pKa = 11.84TSDD41 pKa = 3.03KK42 pKa = 10.66FFLFYY47 pKa = 10.78FRR49 pKa = 11.84VKK51 pKa = 10.66YY52 pKa = 10.15SLEE55 pKa = 3.66RR56 pKa = 11.84AVQNFPYY63 pKa = 10.27VSPLLRR69 pKa = 11.84CSGAEE74 pKa = 3.72LWNSILLIKK83 pKa = 10.51LQFGDD88 pKa = 4.56SNWSRR93 pKa = 11.84IRR95 pKa = 11.84SGDD98 pKa = 3.51LASEE102 pKa = 4.17SSILPKK108 pKa = 10.43RR109 pKa = 11.84QFTEE113 pKa = 4.01VQKK116 pKa = 11.15YY117 pKa = 9.95KK118 pKa = 10.92YY119 pKa = 10.12FEE121 pKa = 4.29CTVFGSDD128 pKa = 3.2VGLTFGASAALRR140 pKa = 11.84ARR142 pKa = 11.84FATLAWPSAHH152 pKa = 6.43FALSLALQSKK162 pKa = 9.92SRR164 pKa = 11.84AKK166 pKa = 10.75SFGLAKK172 pKa = 10.19RR173 pKa = 11.84RR174 pKa = 11.84NTLVVRR180 pKa = 11.84RR181 pKa = 11.84KK182 pKa = 9.91ALNTFDD188 pKa = 5.17VRR190 pKa = 11.84DD191 pKa = 3.61LL192 pKa = 3.91

Molecular weight: 21.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3927

0

3927

1283000

27

3406

326.7

36.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.233 ± 0.034

0.759 ± 0.012

4.982 ± 0.026

7.236 ± 0.044

5.51 ± 0.036

7.08 ± 0.04

1.634 ± 0.017

7.177 ± 0.037

7.49 ± 0.032

10.497 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.896 ± 0.015

4.253 ± 0.03

4.184 ± 0.027

2.984 ± 0.02

4.679 ± 0.03

7.863 ± 0.041

4.861 ± 0.031

5.97 ± 0.033

1.183 ± 0.015

3.526 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski