Escherichia phage 121Q

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Asteriusvirus; Escherichia virus 121Q

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 611 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A097EXQ7|A0A097EXQ7_9CAUD Uncharacterized protein OS=Escherichia phage 121Q OX=1555202 GN=303 PE=4 SV=1
MM1 pKa = 7.85GIQHH5 pKa = 6.55ITRR8 pKa = 11.84TTDD11 pKa = 2.63NGEE14 pKa = 3.6ITGYY18 pKa = 9.49QVQGKK23 pKa = 8.39VAQLQYY29 pKa = 10.13WFVKK33 pKa = 10.2RR34 pKa = 11.84GIVAPDD40 pKa = 3.71LDD42 pKa = 4.07YY43 pKa = 11.75DD44 pKa = 3.79RR45 pKa = 11.84VAFIDD50 pKa = 3.84SLYY53 pKa = 10.7VDD55 pKa = 4.25PDD57 pKa = 3.18NRR59 pKa = 11.84GNGEE63 pKa = 3.84GTEE66 pKa = 4.11LMNAFIDD73 pKa = 4.03AADD76 pKa = 4.66DD77 pKa = 3.69SDD79 pKa = 6.14CIILEE84 pKa = 4.42CDD86 pKa = 2.93TGEE89 pKa = 4.61DD90 pKa = 3.62NEE92 pKa = 4.54FNLKK96 pKa = 8.63EE97 pKa = 4.02WYY99 pKa = 9.69EE100 pKa = 4.11GFGFEE105 pKa = 5.02VLINCEE111 pKa = 4.18YY112 pKa = 10.55PIMILSS118 pKa = 3.64

Molecular weight: 13.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A097EX93|A0A097EX93_9CAUD Uncharacterized protein OS=Escherichia phage 121Q OX=1555202 GN=174 PE=4 SV=1
MM1 pKa = 7.49NSTIRR6 pKa = 11.84RR7 pKa = 11.84IWCKK11 pKa = 9.93LRR13 pKa = 11.84RR14 pKa = 11.84CNPEE18 pKa = 2.97QDD20 pKa = 2.87LWYY23 pKa = 10.14SRR25 pKa = 11.84KK26 pKa = 7.71WW27 pKa = 3.25

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

611

0

611

107219

20

3323

175.5

20.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.858 ± 0.127

1.35 ± 0.054

6.499 ± 0.11

6.714 ± 0.153

4.563 ± 0.079

5.654 ± 0.17

1.887 ± 0.06

7.486 ± 0.103

6.984 ± 0.154

7.914 ± 0.108

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.874 ± 0.067

6.362 ± 0.095

3.061 ± 0.073

3.525 ± 0.075

3.679 ± 0.078

7.072 ± 0.112

6.45 ± 0.201

6.633 ± 0.092

1.077 ± 0.046

5.358 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski