Collinsella sp. An271

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Collinsella; unclassified Collinsella

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2335 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4DG65|A0A1Y4DG65_9ACTN DNA primase OS=Collinsella sp. An271 OX=1965616 GN=dnaG PE=3 SV=1
MM1 pKa = 7.43KK2 pKa = 10.05RR3 pKa = 11.84FHH5 pKa = 6.64SVWRR9 pKa = 11.84ACALSCALVAGLSLAPIIAGATAADD34 pKa = 3.57AAAATDD40 pKa = 5.28GILITLDD47 pKa = 3.35SAASAQARR55 pKa = 11.84SLGGDD60 pKa = 3.58DD61 pKa = 3.94VNLLAEE67 pKa = 4.45SEE69 pKa = 4.72VVAQLGQAGLEE80 pKa = 4.28VNDD83 pKa = 4.14SFEE86 pKa = 4.41SQGGQVVLTADD97 pKa = 3.57TTGDD101 pKa = 3.6LSDD104 pKa = 4.53EE105 pKa = 4.21EE106 pKa = 5.0ALAQARR112 pKa = 11.84DD113 pKa = 3.55IEE115 pKa = 4.64GVADD119 pKa = 3.45VQYY122 pKa = 10.93NYY124 pKa = 10.45VYY126 pKa = 10.46HH127 pKa = 6.63LVDD130 pKa = 5.5IIEE133 pKa = 4.41EE134 pKa = 4.31DD135 pKa = 3.53ATSSTGTSRR144 pKa = 11.84SGVSTLTVSSVNDD157 pKa = 3.57PFAQISSPAADD168 pKa = 3.52NNQYY172 pKa = 9.66WLYY175 pKa = 10.89SANLDD180 pKa = 4.42DD181 pKa = 5.5AWEE184 pKa = 4.1QAQSDD189 pKa = 4.14HH190 pKa = 7.52DD191 pKa = 3.61ITIAVLDD198 pKa = 3.9SGVDD202 pKa = 3.66LDD204 pKa = 5.53HH205 pKa = 7.51EE206 pKa = 4.5DD207 pKa = 3.33LAGNILTDD215 pKa = 4.37LAWDD219 pKa = 3.68ATLEE223 pKa = 4.23QPLSACEE230 pKa = 3.79ITDD233 pKa = 3.94CAGHH237 pKa = 5.65GTMVAGVASAVANNALGIAGASYY260 pKa = 10.87NADD263 pKa = 3.89LLPIKK268 pKa = 10.73VVDD271 pKa = 3.52NEE273 pKa = 3.88EE274 pKa = 4.01RR275 pKa = 11.84QITSADD281 pKa = 3.38LVKK284 pKa = 10.58SYY286 pKa = 10.91EE287 pKa = 4.0YY288 pKa = 11.19LFDD291 pKa = 4.61LVDD294 pKa = 4.37SGRR297 pKa = 11.84LDD299 pKa = 3.48NLRR302 pKa = 11.84VINMSLGGYY311 pKa = 9.15EE312 pKa = 4.5DD313 pKa = 3.94SQANDD318 pKa = 3.21EE319 pKa = 4.65AFHH322 pKa = 6.99DD323 pKa = 4.62VIKK326 pKa = 9.62TALNTYY332 pKa = 10.42DD333 pKa = 4.06ILTVCAGGNGNQTTTPNTGKK353 pKa = 9.76MYY355 pKa = 10.07PADD358 pKa = 4.02FDD360 pKa = 3.83EE361 pKa = 5.9CISVTALEE369 pKa = 4.24SDD371 pKa = 3.59GTNLVWSDD379 pKa = 3.39YY380 pKa = 11.73NEE382 pKa = 4.38FKK384 pKa = 10.79DD385 pKa = 4.88ISAPGQNIRR394 pKa = 11.84TTNMSGGFSTASGTSVASPIVSGTVALLYY423 pKa = 10.74AADD426 pKa = 4.28PDD428 pKa = 3.85ATPEE432 pKa = 3.9QVRR435 pKa = 11.84AALEE439 pKa = 4.05EE440 pKa = 4.13TAVPVVDD447 pKa = 4.38EE448 pKa = 4.17EE449 pKa = 4.28HH450 pKa = 7.36DD451 pKa = 3.61RR452 pKa = 11.84TEE454 pKa = 4.12VSGSAGALDD463 pKa = 3.7AGAAVEE469 pKa = 4.06QLTGAVEE476 pKa = 4.22PEE478 pKa = 4.55PEE480 pKa = 4.85PDD482 pKa = 4.01PDD484 pKa = 4.18PEE486 pKa = 4.77PEE488 pKa = 4.44PEE490 pKa = 4.27LPFVDD495 pKa = 5.4VDD497 pKa = 3.9HH498 pKa = 6.69EE499 pKa = 4.53WYY501 pKa = 10.42YY502 pKa = 10.03PAIVYY507 pKa = 9.95VYY509 pKa = 9.96EE510 pKa = 3.94NDD512 pKa = 3.33IMHH515 pKa = 7.28GLSDD519 pKa = 4.08SPRR522 pKa = 11.84FAPNEE527 pKa = 4.18PIVRR531 pKa = 11.84QDD533 pKa = 3.3AAVVLYY539 pKa = 10.76NILGNGEE546 pKa = 4.15KK547 pKa = 10.66APDD550 pKa = 4.07CGLVDD555 pKa = 3.88VGQDD559 pKa = 3.53YY560 pKa = 8.18YY561 pKa = 11.44TDD563 pKa = 3.47AVNWAVAHH571 pKa = 6.43GYY573 pKa = 9.81INGYY577 pKa = 9.35EE578 pKa = 3.94NVATGEE584 pKa = 4.2VTHH587 pKa = 6.7FGVGDD592 pKa = 3.83PLTRR596 pKa = 11.84EE597 pKa = 3.69QLACIVANIARR608 pKa = 11.84NGEE611 pKa = 4.27PFGSMAKK618 pKa = 9.8YY619 pKa = 10.76EE620 pKa = 4.34SMPDD624 pKa = 3.23HH625 pKa = 7.65DD626 pKa = 5.36KK627 pKa = 10.75TSTWAVTNVVWAINNQIINGVEE649 pKa = 3.95LGDD652 pKa = 3.63VRR654 pKa = 11.84YY655 pKa = 9.44VAPQNKK661 pKa = 7.64ATRR664 pKa = 11.84AEE666 pKa = 4.03MAAIMMNCLEE676 pKa = 3.98QDD678 pKa = 3.71VFF680 pKa = 4.18

Molecular weight: 72.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4DQP6|A0A1Y4DQP6_9ACTN Uncharacterized protein OS=Collinsella sp. An271 OX=1965616 GN=B5F74_04790 PE=4 SV=1
MM1 pKa = 7.41IRR3 pKa = 11.84RR4 pKa = 11.84NATKK8 pKa = 10.41RR9 pKa = 11.84NTLSSPGRR17 pKa = 11.84RR18 pKa = 11.84AAWYY22 pKa = 10.16GLLTALALCAGYY34 pKa = 10.88LEE36 pKa = 4.56VLVPLPVSVPGIKK49 pKa = 10.16LGLGNAVVLFALEE62 pKa = 4.0RR63 pKa = 11.84MGARR67 pKa = 11.84PALFIMLAKK76 pKa = 9.62VFCSMLLFSTPQMLAFSLGGGLLSWAIMAAAVRR109 pKa = 11.84LDD111 pKa = 3.63LFSEE115 pKa = 4.42VGVSVLGGVAHH126 pKa = 7.0NAGQLLVVAALLSPQVALVNAPVLAIAGVACGLAIGLILRR166 pKa = 11.84GVLAALPKK174 pKa = 10.6AGFRR178 pKa = 11.84EE179 pKa = 4.34

Molecular weight: 18.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2335

0

2335

779385

37

2360

333.8

36.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.846 ± 0.081

1.585 ± 0.023

6.495 ± 0.037

6.568 ± 0.048

3.537 ± 0.031

8.249 ± 0.045

1.948 ± 0.023

5.3 ± 0.041

3.118 ± 0.042

9.14 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.716 ± 0.023

2.644 ± 0.031

4.357 ± 0.033

2.774 ± 0.03

6.633 ± 0.063

5.828 ± 0.036

5.337 ± 0.038

8.017 ± 0.043

1.07 ± 0.019

2.84 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski