Phreatobacter cathodiphilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phreatobacteraceae; Phreatobacter

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4219 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S0NBL6|A0A2S0NBL6_9PROT Asp/Glu/hydantoin racemase OS=Phreatobacter cathodiphilus OX=1868589 GN=C6569_11050 PE=4 SV=1
MM1 pKa = 7.69CNVCSGPMLRR11 pKa = 11.84DD12 pKa = 3.22WTGKK16 pKa = 7.04TVHH19 pKa = 6.71SNTGGQDD26 pKa = 3.3GPGDD30 pKa = 3.63VSFKK34 pKa = 11.04GLTGGMLQSIYY45 pKa = 11.07GGITGGVSQGVQQPTGGVSQGVTIGDD71 pKa = 3.84PTGGTAAEE79 pKa = 4.31PASGSYY85 pKa = 9.68SQSATGNTNIDD96 pKa = 4.45GILSGYY102 pKa = 9.99KK103 pKa = 7.98WTTTALTWRR112 pKa = 11.84IPTSADD118 pKa = 3.78QYY120 pKa = 10.92DD121 pKa = 4.49TDD123 pKa = 4.89PGTAGIQYY131 pKa = 10.48GDD133 pKa = 3.81TARR136 pKa = 11.84TSTFLAPTAAMTTATAEE153 pKa = 4.29ILNQMFGAVSGLSFTQAAASDD174 pKa = 3.8TTADD178 pKa = 3.51MSIGRR183 pKa = 11.84STASPSTNFNTAYY196 pKa = 10.19AYY198 pKa = 10.47YY199 pKa = 9.63PSGPGTGLSGDD210 pKa = 3.21SWYY213 pKa = 11.23SDD215 pKa = 3.23AYY217 pKa = 10.09EE218 pKa = 4.42SFGSGYY224 pKa = 10.85AYY226 pKa = 10.77SNPVKK231 pKa = 10.46GGYY234 pKa = 9.69AWTTYY239 pKa = 8.06IHH241 pKa = 7.18EE242 pKa = 4.77YY243 pKa = 8.01GHH245 pKa = 6.71NMGLKK250 pKa = 9.88HH251 pKa = 5.62GHH253 pKa = 4.39QTGGPGNTAMSADD266 pKa = 4.23RR267 pKa = 11.84DD268 pKa = 3.95SMEE271 pKa = 4.28FSVMTYY277 pKa = 9.95RR278 pKa = 11.84SYY280 pKa = 11.41VGQDD284 pKa = 2.88TVANPGYY291 pKa = 10.47QNEE294 pKa = 4.24QWGFAQSLMMYY305 pKa = 9.94DD306 pKa = 3.52IAALQVMYY314 pKa = 10.57GADD317 pKa = 3.25FTTNNTNTVYY327 pKa = 10.29TFSATTGQMFINGVGQGAPGGNRR350 pKa = 11.84VFLTIWDD357 pKa = 3.75GGGIDD362 pKa = 4.15TYY364 pKa = 11.63DD365 pKa = 3.4FSNYY369 pKa = 8.91TGNQTIDD376 pKa = 3.7LTPGSWSLMSTTQRR390 pKa = 11.84AHH392 pKa = 7.24LGQGNYY398 pKa = 10.15ARR400 pKa = 11.84GNVFNALQYY409 pKa = 10.78NGDD412 pKa = 3.52VRR414 pKa = 11.84SLIEE418 pKa = 3.82NAIGGSGNDD427 pKa = 3.81TITGNAADD435 pKa = 3.77NTLTGGAGADD445 pKa = 3.58TLIGGGGSDD454 pKa = 3.31TASYY458 pKa = 8.27ATSTSRR464 pKa = 11.84VVANLADD471 pKa = 3.81SSINTGDD478 pKa = 3.67AAGDD482 pKa = 3.74TYY484 pKa = 11.74SSIEE488 pKa = 4.03NLLGTNVSGVGDD500 pKa = 3.81ALVGNSGRR508 pKa = 11.84NFLEE512 pKa = 4.11GLGGNDD518 pKa = 3.3QLFGRR523 pKa = 11.84AGDD526 pKa = 3.72DD527 pKa = 3.36VLYY530 pKa = 11.08GGAGDD535 pKa = 4.99DD536 pKa = 4.4LLLGGLGADD545 pKa = 3.57WLDD548 pKa = 3.7GGTDD552 pKa = 3.58TDD554 pKa = 3.9TVSYY558 pKa = 10.77QEE560 pKa = 4.44AGSGVVVDD568 pKa = 4.85MLNASIQTGEE578 pKa = 3.96AAGDD582 pKa = 3.5QLISIEE588 pKa = 4.44NIFGSSFDD596 pKa = 5.35DD597 pKa = 4.05ALCGNNGRR605 pKa = 11.84NFLYY609 pKa = 10.47GQDD612 pKa = 3.84GNDD615 pKa = 3.22VLFGRR620 pKa = 11.84GGADD624 pKa = 3.1VLYY627 pKa = 10.37GGAGADD633 pKa = 3.65TLFGGAGSDD642 pKa = 3.38WLDD645 pKa = 3.31GGAGFDD651 pKa = 3.55YY652 pKa = 10.96ASYY655 pKa = 10.74LDD657 pKa = 4.38AGAAVQVDD665 pKa = 4.22MNNPAINLGDD675 pKa = 3.63AAGDD679 pKa = 3.47QLIDD683 pKa = 3.34IEE685 pKa = 4.6GLVGSGFDD693 pKa = 5.68DD694 pKa = 3.56VLLGNNLGNAIFGADD709 pKa = 4.0GNDD712 pKa = 3.38VIYY715 pKa = 10.87GRR717 pKa = 11.84GGGDD721 pKa = 3.75YY722 pKa = 10.1LTGGAGADD730 pKa = 3.15QFRR733 pKa = 11.84FANAGDD739 pKa = 4.1AGDD742 pKa = 4.63TISDD746 pKa = 4.03FQRR749 pKa = 11.84GLDD752 pKa = 3.52TLQFSASGFGGGLAGGALAANRR774 pKa = 11.84LVLGTAATQNFGQFIFDD791 pKa = 3.43SSTSILYY798 pKa = 9.44WDD800 pKa = 4.46ADD802 pKa = 3.78GTGAGAKK809 pKa = 8.98IAMMALSNINTLSTSDD825 pKa = 3.75FLINAA830 pKa = 4.81

Molecular weight: 84.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S0NII3|A0A2S0NII3_9PROT Methyltransferase type 11 OS=Phreatobacter cathodiphilus OX=1868589 GN=C6569_20615 PE=4 SV=1
MM1 pKa = 7.65APPRR5 pKa = 11.84PRR7 pKa = 11.84RR8 pKa = 11.84WSVRR12 pKa = 11.84RR13 pKa = 11.84WPRR16 pKa = 11.84APRR19 pKa = 11.84AGPRR23 pKa = 11.84RR24 pKa = 11.84PILRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84ARR32 pKa = 11.84CRR34 pKa = 11.84ASSPRR39 pKa = 11.84SSRR42 pKa = 11.84PRR44 pKa = 11.84LSRR47 pKa = 11.84LRR49 pKa = 11.84PSPLRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84PPRR59 pKa = 11.84PTRR62 pKa = 11.84TKK64 pKa = 10.84LSAA67 pKa = 3.58

Molecular weight: 8.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4219

0

4219

1331878

29

2125

315.7

33.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.875 ± 0.06

0.776 ± 0.011

5.37 ± 0.031

5.373 ± 0.038

3.662 ± 0.026

9.012 ± 0.034

1.924 ± 0.018

4.868 ± 0.027

2.796 ± 0.031

9.916 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.519 ± 0.016

2.167 ± 0.02

5.615 ± 0.037

2.839 ± 0.025

7.711 ± 0.04

4.803 ± 0.026

5.449 ± 0.025

8.01 ± 0.027

1.323 ± 0.015

1.992 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski