Blackcurrant-associated closterovirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Closterovirus; unclassified Closterovirus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385L386|A0A385L386_9CLOS 11 kDa protein OS=Blackcurrant-associated closterovirus 1 OX=2338394 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 4.85SYY4 pKa = 12.03GGATNNKK11 pKa = 9.09SATIFIDD18 pKa = 5.3RR19 pKa = 11.84EE20 pKa = 4.37CSWLGYY26 pKa = 9.8RR27 pKa = 11.84EE28 pKa = 4.02SLIDD32 pKa = 3.66KK33 pKa = 10.76GRR35 pKa = 11.84MCLVDD40 pKa = 5.81DD41 pKa = 5.05AYY43 pKa = 11.57SWDD46 pKa = 3.41GVEE49 pKa = 5.36ISEE52 pKa = 4.61TGNGEE57 pKa = 4.02YY58 pKa = 9.99TLSEE62 pKa = 4.32FFDD65 pKa = 3.85SCLVEE70 pKa = 4.49PGVQVLNIGKK80 pKa = 9.25NDD82 pKa = 4.17SISTNVLHH90 pKa = 7.26DD91 pKa = 4.33FSSTFTIYY99 pKa = 10.31HH100 pKa = 5.36GCLRR104 pKa = 11.84RR105 pKa = 11.84TVVTFDD111 pKa = 3.28SDD113 pKa = 3.99PVVNRR118 pKa = 11.84SILCNDD124 pKa = 3.97CSPVKK129 pKa = 10.76SLVDD133 pKa = 3.54LLGSVKK139 pKa = 9.64SWRR142 pKa = 11.84LKK144 pKa = 10.27QITMVNLL151 pKa = 3.38

Molecular weight: 16.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385L378|A0A385L378_9CLOS 61 kDa protein OS=Blackcurrant-associated closterovirus 1 OX=2338394 PE=4 SV=1
MM1 pKa = 8.2DD2 pKa = 5.72CGLKK6 pKa = 10.41FLLLFFFGDD15 pKa = 3.76VVFVTFFLFLFFAVRR30 pKa = 11.84RR31 pKa = 11.84PARR34 pKa = 11.84DD35 pKa = 3.39NRR37 pKa = 11.84DD38 pKa = 3.46LEE40 pKa = 4.14SSARR44 pKa = 11.84VTVRR48 pKa = 3.19

Molecular weight: 5.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

5372

48

3311

596.9

66.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.162 ± 0.297

2.587 ± 0.335

5.92 ± 0.366

5.771 ± 0.431

5.473 ± 0.586

5.957 ± 0.495

2.122 ± 0.408

5.417 ± 0.162

6.404 ± 0.371

9.81 ± 0.561

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.843 ± 0.139

4.635 ± 0.516

3.276 ± 0.209

2.457 ± 0.196

5.994 ± 0.165

9.159 ± 0.643

5.398 ± 0.367

7.558 ± 0.247

0.652 ± 0.121

3.407 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski