Sphingomonas phage PAU

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 295 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9NCD2|H9NCD2_9CAUD Gp255 OS=Sphingomonas phage PAU OX=1150991 GN=255 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.1PEE4 pKa = 4.08NFDD7 pKa = 3.3IEE9 pKa = 4.52KK10 pKa = 10.67VKK12 pKa = 10.75EE13 pKa = 4.02HH14 pKa = 6.25LQGTCMSLDD23 pKa = 3.48EE24 pKa = 5.05GLSDD28 pKa = 5.57LYY30 pKa = 10.72PDD32 pKa = 3.76MDD34 pKa = 3.8QFDD37 pKa = 5.54LEE39 pKa = 4.48MSDD42 pKa = 4.72HH43 pKa = 6.35EE44 pKa = 5.42AIDD47 pKa = 3.68MEE49 pKa = 4.43MFRR52 pKa = 11.84CDD54 pKa = 3.11GCGWWFDD61 pKa = 3.47NSEE64 pKa = 4.08MGEE67 pKa = 4.33EE68 pKa = 4.14EE69 pKa = 4.39CQDD72 pKa = 3.81CEE74 pKa = 4.11EE75 pKa = 4.55TEE77 pKa = 4.03

Molecular weight: 9.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9NBX7|H9NBX7_9CAUD Gp100 OS=Sphingomonas phage PAU OX=1150991 GN=100 PE=4 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84KK3 pKa = 8.54MNRR6 pKa = 11.84SEE8 pKa = 4.59LYY10 pKa = 10.22FRR12 pKa = 11.84LNTALKK18 pKa = 10.57GVVTTRR24 pKa = 11.84GISYY28 pKa = 10.23SVNVFNDD35 pKa = 3.0NTGIVQVNVSISNMRR50 pKa = 11.84ASVLACVKK58 pKa = 10.62EE59 pKa = 4.07NDD61 pKa = 3.63DD62 pKa = 4.26GTWSVSDD69 pKa = 3.44NSYY72 pKa = 10.3TIVVDD77 pKa = 3.44NLNQVGSTIIRR88 pKa = 11.84MVTKK92 pKa = 10.28IRR94 pKa = 11.84TLRR97 pKa = 11.84SRR99 pKa = 11.84INTT102 pKa = 3.66

Molecular weight: 11.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

295

0

295

69479

45

1857

235.5

27.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.267 ± 0.186

0.938 ± 0.065

6.494 ± 0.127

7.248 ± 0.205

5.171 ± 0.128

4.311 ± 0.178

1.873 ± 0.077

8.139 ± 0.131

8.184 ± 0.187

9.042 ± 0.133

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.5 ± 0.094

7.266 ± 0.158

2.745 ± 0.089

2.949 ± 0.079

3.824 ± 0.094

7.666 ± 0.155

6.166 ± 0.125

5.737 ± 0.111

0.94 ± 0.038

4.541 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski