Amino acid dipepetide frequency for Rhodotorula graminis (strain WP1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.148AlaAla: 18.148 ± 0.152
1.137AlaCys: 1.137 ± 0.023
5.85AlaAsp: 5.85 ± 0.041
5.574AlaGlu: 5.574 ± 0.049
3.675AlaPhe: 3.675 ± 0.036
8.745AlaGly: 8.745 ± 0.065
3.068AlaHis: 3.068 ± 0.033
3.268AlaIle: 3.268 ± 0.037
4.239AlaLys: 4.239 ± 0.048
11.506AlaLeu: 11.506 ± 0.081
1.854AlaMet: 1.854 ± 0.025
2.338AlaAsn: 2.338 ± 0.026
9.754AlaPro: 9.754 ± 0.098
4.599AlaGln: 4.599 ± 0.046
8.773AlaArg: 8.773 ± 0.072
11.027AlaSer: 11.027 ± 0.092
6.453AlaThr: 6.453 ± 0.05
7.226AlaVal: 7.226 ± 0.05
1.337AlaTrp: 1.337 ± 0.021
2.252AlaTyr: 2.252 ± 0.028
0.0AlaXaa: 0.0 ± 0.0
Cys
1.155CysAla: 1.155 ± 0.023
0.18CysCys: 0.18 ± 0.007
0.573CysAsp: 0.573 ± 0.012
0.491CysGlu: 0.491 ± 0.014
0.388CysPhe: 0.388 ± 0.012
0.877CysGly: 0.877 ± 0.018
0.22CysHis: 0.22 ± 0.009
0.429CysIle: 0.429 ± 0.011
0.328CysLys: 0.328 ± 0.01
0.954CysLeu: 0.954 ± 0.017
0.172CysMet: 0.172 ± 0.01
0.233CysAsn: 0.233 ± 0.008
0.592CysPro: 0.592 ± 0.014
0.264CysGln: 0.264 ± 0.009
0.711CysArg: 0.711 ± 0.017
0.721CysSer: 0.721 ± 0.017
0.586CysThr: 0.586 ± 0.014
0.742CysVal: 0.742 ± 0.015
0.144CysTrp: 0.144 ± 0.006
0.245CysTyr: 0.245 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
6.508AspAla: 6.508 ± 0.049
0.469AspCys: 0.469 ± 0.013
6.126AspAsp: 6.126 ± 0.079
6.019AspGlu: 6.019 ± 0.063
1.766AspPhe: 1.766 ± 0.024
4.766AspGly: 4.766 ± 0.05
1.115AspHis: 1.115 ± 0.018
1.711AspIle: 1.711 ± 0.025
2.556AspLys: 2.556 ± 0.03
5.554AspLeu: 5.554 ± 0.046
0.905AspMet: 0.905 ± 0.015
1.151AspAsn: 1.151 ± 0.017
3.622AspPro: 3.622 ± 0.038
1.567AspGln: 1.567 ± 0.02
3.152AspArg: 3.152 ± 0.036
3.788AspSer: 3.788 ± 0.035
2.289AspThr: 2.289 ± 0.024
3.996AspVal: 3.996 ± 0.034
0.778AspTrp: 0.778 ± 0.015
1.218AspTyr: 1.218 ± 0.018
0.0AspXaa: 0.0 ± 0.0
Glu
6.096GluAla: 6.096 ± 0.053
0.518GluCys: 0.518 ± 0.013
3.777GluAsp: 3.777 ± 0.043
4.27GluGlu: 4.27 ± 0.048
1.446GluPhe: 1.446 ± 0.019
4.851GluGly: 4.851 ± 0.053
1.547GluHis: 1.547 ± 0.021
1.775GluIle: 1.775 ± 0.024
2.162GluLys: 2.162 ± 0.027
5.577GluLeu: 5.577 ± 0.046
1.058GluMet: 1.058 ± 0.017
1.1GluAsn: 1.1 ± 0.019
3.117GluPro: 3.117 ± 0.04
2.517GluGln: 2.517 ± 0.033
5.559GluArg: 5.559 ± 0.05
3.081GluSer: 3.081 ± 0.031
2.506GluThr: 2.506 ± 0.026
3.802GluVal: 3.802 ± 0.033
0.892GluTrp: 0.892 ± 0.015
1.242GluTyr: 1.242 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
3.707PheAla: 3.707 ± 0.035
0.438PheCys: 0.438 ± 0.011
2.182PheAsp: 2.182 ± 0.026
1.857PheGlu: 1.857 ± 0.026
1.329PhePhe: 1.329 ± 0.024
2.633PheGly: 2.633 ± 0.037
0.742PheHis: 0.742 ± 0.014
1.122PheIle: 1.122 ± 0.021
1.139PheLys: 1.139 ± 0.019
3.228PheLeu: 3.228 ± 0.039
0.471PheMet: 0.471 ± 0.013
0.895PheAsn: 0.895 ± 0.018
1.834PhePro: 1.834 ± 0.025
0.877PheGln: 0.877 ± 0.015
1.706PheArg: 1.706 ± 0.024
2.608PheSer: 2.608 ± 0.032
1.624PheThr: 1.624 ± 0.024
2.446PheVal: 2.446 ± 0.03
0.435PheTrp: 0.435 ± 0.012
0.807PheTyr: 0.807 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
9.449GlyAla: 9.449 ± 0.076
0.679GlyCys: 0.679 ± 0.015
4.327GlyAsp: 4.327 ± 0.04
4.679GlyGlu: 4.679 ± 0.045
2.451GlyPhe: 2.451 ± 0.034
9.257GlyGly: 9.257 ± 0.117
1.603GlyHis: 1.603 ± 0.025
2.437GlyIle: 2.437 ± 0.031
3.387GlyLys: 3.387 ± 0.04
6.071GlyLeu: 6.071 ± 0.053
1.296GlyMet: 1.296 ± 0.025
1.556GlyAsn: 1.556 ± 0.024
4.043GlyPro: 4.043 ± 0.04
2.449GlyGln: 2.449 ± 0.03
5.258GlyArg: 5.258 ± 0.048
5.864GlySer: 5.864 ± 0.06
3.823GlyThr: 3.823 ± 0.038
4.76GlyVal: 4.76 ± 0.039
1.154GlyTrp: 1.154 ± 0.022
1.65GlyTyr: 1.65 ± 0.029
0.0GlyXaa: 0.0 ± 0.0
His
2.794HisAla: 2.794 ± 0.036
0.241HisCys: 0.241 ± 0.009
1.549HisAsp: 1.549 ± 0.023
1.348HisGlu: 1.348 ± 0.021
0.804HisPhe: 0.804 ± 0.016
1.851HisGly: 1.851 ± 0.029
0.935HisHis: 0.935 ± 0.022
0.709HisIle: 0.709 ± 0.017
0.778HisLys: 0.778 ± 0.016
2.779HisLeu: 2.779 ± 0.027
0.319HisMet: 0.319 ± 0.013
0.519HisAsn: 0.519 ± 0.011
1.971HisPro: 1.971 ± 0.027
0.827HisGln: 0.827 ± 0.02
1.611HisArg: 1.611 ± 0.023
1.659HisSer: 1.659 ± 0.024
1.043HisThr: 1.043 ± 0.019
1.645HisVal: 1.645 ± 0.024
0.287HisTrp: 0.287 ± 0.016
0.557HisTyr: 0.557 ± 0.012
0.0HisXaa: 0.0 ± 0.0
Ile
3.233IleAla: 3.233 ± 0.034
0.418IleCys: 0.418 ± 0.011
1.982IleAsp: 1.982 ± 0.024
1.911IleGlu: 1.911 ± 0.027
1.155IlePhe: 1.155 ± 0.023
2.173IleGly: 2.173 ± 0.03
0.668IleHis: 0.668 ± 0.014
1.201IleIle: 1.201 ± 0.024
1.387IleLys: 1.387 ± 0.03
3.051IleLeu: 3.051 ± 0.042
0.537IleMet: 0.537 ± 0.014
0.898IleAsn: 0.898 ± 0.02
1.846IlePro: 1.846 ± 0.025
0.974IleGln: 0.974 ± 0.018
1.837IleArg: 1.837 ± 0.025
2.229IleSer: 2.229 ± 0.029
1.544IleThr: 1.544 ± 0.027
2.51IleVal: 2.51 ± 0.031
0.395IleTrp: 0.395 ± 0.011
0.746IleTyr: 0.746 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
4.248LysAla: 4.248 ± 0.049
0.315LysCys: 0.315 ± 0.011
2.127LysAsp: 2.127 ± 0.03
2.423LysGlu: 2.423 ± 0.031
0.952LysPhe: 0.952 ± 0.018
3.075LysGly: 3.075 ± 0.04
0.864LysHis: 0.864 ± 0.016
1.247LysIle: 1.247 ± 0.024
2.596LysLys: 2.596 ± 0.05
3.367LysLeu: 3.367 ± 0.037
0.691LysMet: 0.691 ± 0.016
0.919LysAsn: 0.919 ± 0.017
2.211LysPro: 2.211 ± 0.031
1.399LysGln: 1.399 ± 0.025
3.213LysArg: 3.213 ± 0.038
2.23LysSer: 2.23 ± 0.031
1.907LysThr: 1.907 ± 0.027
2.67LysVal: 2.67 ± 0.036
0.47LysTrp: 0.47 ± 0.013
0.817LysTyr: 0.817 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
11.864LeuAla: 11.864 ± 0.084
1.1LeuCys: 1.1 ± 0.023
6.226LeuAsp: 6.226 ± 0.044
5.861LeuGlu: 5.861 ± 0.055
3.234LeuPhe: 3.234 ± 0.038
6.613LeuGly: 6.613 ± 0.045
2.201LeuHis: 2.201 ± 0.027
2.665LeuIle: 2.665 ± 0.036
3.425LeuLys: 3.425 ± 0.037
8.729LeuLeu: 8.729 ± 0.079
1.276LeuMet: 1.276 ± 0.028
2.205LeuAsn: 2.205 ± 0.028
6.44LeuPro: 6.44 ± 0.052
2.836LeuGln: 2.836 ± 0.033
6.439LeuArg: 6.439 ± 0.049
8.034LeuSer: 8.034 ± 0.059
4.217LeuThr: 4.217 ± 0.04
7.68LeuVal: 7.68 ± 0.067
1.074LeuTrp: 1.074 ± 0.021
2.111LeuTyr: 2.111 ± 0.027
0.0LeuXaa: 0.0 ± 0.0
Met
1.755MetAla: 1.755 ± 0.023
0.182MetCys: 0.182 ± 0.007
0.869MetAsp: 0.869 ± 0.016
0.689MetGlu: 0.689 ± 0.02
0.475MetPhe: 0.475 ± 0.014
1.153MetGly: 1.153 ± 0.022
0.385MetHis: 0.385 ± 0.013
0.506MetIle: 0.506 ± 0.013
0.491MetLys: 0.491 ± 0.014
1.48MetLeu: 1.48 ± 0.021
0.322MetMet: 0.322 ± 0.011
0.371MetAsn: 0.371 ± 0.011
1.023MetPro: 1.023 ± 0.017
0.607MetGln: 0.607 ± 0.014
1.206MetArg: 1.206 ± 0.019
1.481MetSer: 1.481 ± 0.023
0.829MetThr: 0.829 ± 0.016
0.93MetVal: 0.93 ± 0.016
0.193MetTrp: 0.193 ± 0.007
0.359MetTyr: 0.359 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.337AsnAla: 2.337 ± 0.028
0.244AsnCys: 0.244 ± 0.009
1.238AsnAsp: 1.238 ± 0.017
1.268AsnGlu: 1.268 ± 0.021
0.785AsnPhe: 0.785 ± 0.018
1.857AsnGly: 1.857 ± 0.029
0.415AsnHis: 0.415 ± 0.011
0.852AsnIle: 0.852 ± 0.02
0.955AsnLys: 0.955 ± 0.019
2.29AsnLeu: 2.29 ± 0.028
0.373AsnMet: 0.373 ± 0.011
0.567AsnAsn: 0.567 ± 0.016
1.643AsnPro: 1.643 ± 0.024
0.64AsnGln: 0.64 ± 0.015
1.207AsnArg: 1.207 ± 0.016
1.499AsnSer: 1.499 ± 0.022
1.047AsnThr: 1.047 ± 0.019
1.638AsnVal: 1.638 ± 0.024
0.315AsnTrp: 0.315 ± 0.01
0.576AsnTyr: 0.576 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
9.303ProAla: 9.303 ± 0.094
0.452ProCys: 0.452 ± 0.011
3.348ProAsp: 3.348 ± 0.033
2.935ProGlu: 2.935 ± 0.032
2.159ProPhe: 2.159 ± 0.024
4.207ProGly: 4.207 ± 0.04
2.027ProHis: 2.027 ± 0.033
1.726ProIle: 1.726 ± 0.025
1.905ProLys: 1.905 ± 0.027
6.13ProLeu: 6.13 ± 0.046
0.773ProMet: 0.773 ± 0.014
1.317ProAsn: 1.317 ± 0.023
8.816ProPro: 8.816 ± 0.126
2.311ProGln: 2.311 ± 0.036
4.847ProArg: 4.847 ± 0.06
8.848ProSer: 8.848 ± 0.078
4.623ProThr: 4.623 ± 0.045
4.464ProVal: 4.464 ± 0.047
0.606ProTrp: 0.606 ± 0.013
1.395ProTyr: 1.395 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.873GlnAla: 3.873 ± 0.036
0.318GlnCys: 0.318 ± 0.011
1.708GlnAsp: 1.708 ± 0.023
1.686GlnGlu: 1.686 ± 0.024
0.932GlnPhe: 0.932 ± 0.019
2.541GlnGly: 2.541 ± 0.031
1.218GlnHis: 1.218 ± 0.023
1.06GlnIle: 1.06 ± 0.022
1.08GlnLys: 1.08 ± 0.021
3.577GlnLeu: 3.577 ± 0.038
0.584GlnMet: 0.584 ± 0.014
0.694GlnAsn: 0.694 ± 0.012
2.465GlnPro: 2.465 ± 0.038
2.866GlnGln: 2.866 ± 0.08
2.74GlnArg: 2.74 ± 0.032
2.1GlnSer: 2.1 ± 0.03
1.463GlnThr: 1.463 ± 0.026
2.158GlnVal: 2.158 ± 0.03
0.394GlnTrp: 0.394 ± 0.01
0.782GlnTyr: 0.782 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
8.592ArgAla: 8.592 ± 0.078
0.693ArgCys: 0.693 ± 0.014
4.079ArgAsp: 4.079 ± 0.046
4.386ArgGlu: 4.386 ± 0.043
2.125ArgPhe: 2.125 ± 0.025
4.665ArgGly: 4.665 ± 0.052
1.72ArgHis: 1.72 ± 0.026
2.316ArgIle: 2.316 ± 0.025
3.006ArgLys: 3.006 ± 0.037
6.572ArgLeu: 6.572 ± 0.056
1.129ArgMet: 1.129 ± 0.022
1.567ArgAsn: 1.567 ± 0.024
5.033ArgPro: 5.033 ± 0.061
2.492ArgGln: 2.492 ± 0.032
7.918ArgArg: 7.918 ± 0.121
5.578ArgSer: 5.578 ± 0.06
3.937ArgThr: 3.937 ± 0.04
4.304ArgVal: 4.304 ± 0.037
1.015ArgTrp: 1.015 ± 0.016
1.417ArgTyr: 1.417 ± 0.018
0.0ArgXaa: 0.0 ± 0.0
Ser
10.17SerAla: 10.17 ± 0.093
0.758SerCys: 0.758 ± 0.018
3.804SerAsp: 3.804 ± 0.036
2.937SerGlu: 2.937 ± 0.03
2.944SerPhe: 2.944 ± 0.033
5.764SerGly: 5.764 ± 0.053
1.926SerHis: 1.926 ± 0.025
2.536SerIle: 2.536 ± 0.03
2.348SerLys: 2.348 ± 0.034
8.035SerLeu: 8.035 ± 0.062
1.196SerMet: 1.196 ± 0.017
1.746SerAsn: 1.746 ± 0.022
7.48SerPro: 7.48 ± 0.084
2.181SerGln: 2.181 ± 0.029
5.73SerArg: 5.73 ± 0.062
13.773SerSer: 13.773 ± 0.144
7.041SerThr: 7.041 ± 0.067
4.368SerVal: 4.368 ± 0.036
0.946SerTrp: 0.946 ± 0.019
1.676SerTyr: 1.676 ± 0.022
0.0SerXaa: 0.0 ± 0.0
Thr
6.489ThrAla: 6.489 ± 0.05
0.63ThrCys: 0.63 ± 0.017
2.427ThrAsp: 2.427 ± 0.027
2.03ThrGlu: 2.03 ± 0.026
1.992ThrPhe: 1.992 ± 0.023
3.696ThrGly: 3.696 ± 0.036
1.147ThrHis: 1.147 ± 0.017
1.884ThrIle: 1.884 ± 0.027
1.837ThrLys: 1.837 ± 0.025
5.435ThrLeu: 5.435 ± 0.051
0.773ThrMet: 0.773 ± 0.014
1.139ThrAsn: 1.139 ± 0.019
4.221ThrPro: 4.221 ± 0.044
1.432ThrGln: 1.432 ± 0.022
3.458ThrArg: 3.458 ± 0.039
6.122ThrSer: 6.122 ± 0.056
3.95ThrThr: 3.95 ± 0.046
3.359ThrVal: 3.359 ± 0.033
0.723ThrTrp: 0.723 ± 0.016
1.214ThrTyr: 1.214 ± 0.017
0.0ThrXaa: 0.0 ± 0.0
Val
7.536ValAla: 7.536 ± 0.053
0.764ValCys: 0.764 ± 0.017
4.637ValAsp: 4.637 ± 0.04
4.678ValGlu: 4.678 ± 0.039
2.199ValPhe: 2.199 ± 0.027
4.892ValGly: 4.892 ± 0.039
1.583ValHis: 1.583 ± 0.022
2.023ValIle: 2.023 ± 0.031
2.896ValLys: 2.896 ± 0.037
6.333ValLeu: 6.333 ± 0.055
0.983ValMet: 0.983 ± 0.018
1.563ValAsn: 1.563 ± 0.028
4.282ValPro: 4.282 ± 0.041
2.273ValGln: 2.273 ± 0.03
4.675ValArg: 4.675 ± 0.046
4.171ValSer: 4.171 ± 0.038
3.087ValThr: 3.087 ± 0.03
5.67ValVal: 5.67 ± 0.046
0.918ValTrp: 0.918 ± 0.021
1.465ValTyr: 1.465 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.317TrpAla: 1.317 ± 0.021
0.169TrpCys: 0.169 ± 0.007
0.833TrpAsp: 0.833 ± 0.016
0.692TrpGlu: 0.692 ± 0.014
0.421TrpPhe: 0.421 ± 0.01
0.778TrpGly: 0.778 ± 0.016
0.306TrpHis: 0.306 ± 0.009
0.501TrpIle: 0.501 ± 0.013
0.462TrpLys: 0.462 ± 0.013
1.236TrpLeu: 1.236 ± 0.022
0.255TrpMet: 0.255 ± 0.008
0.369TrpAsn: 0.369 ± 0.012
0.542TrpPro: 0.542 ± 0.019
0.428TrpGln: 0.428 ± 0.01
1.068TrpArg: 1.068 ± 0.02
0.956TrpSer: 0.956 ± 0.018
0.855TrpThr: 0.855 ± 0.017
0.862TrpVal: 0.862 ± 0.017
0.251TrpTrp: 0.251 ± 0.009
0.315TrpTyr: 0.315 ± 0.01
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.247TyrAla: 2.247 ± 0.031
0.297TyrCys: 0.297 ± 0.011
1.394TyrAsp: 1.394 ± 0.019
1.159TyrGlu: 1.159 ± 0.019
0.848TyrPhe: 0.848 ± 0.017
1.801TyrGly: 1.801 ± 0.029
0.536TyrHis: 0.536 ± 0.014
0.728TyrIle: 0.728 ± 0.017
0.765TyrLys: 0.765 ± 0.015
2.327TyrLeu: 2.327 ± 0.028
0.339TyrMet: 0.339 ± 0.011
0.601TyrAsn: 0.601 ± 0.015
1.257TyrPro: 1.257 ± 0.019
0.7TyrGln: 0.7 ± 0.017
1.435TyrArg: 1.435 ± 0.021
1.592TyrSer: 1.592 ± 0.025
1.132TyrThr: 1.132 ± 0.021
1.398TyrVal: 1.398 ± 0.02
0.287TyrTrp: 0.287 ± 0.01
0.619TyrTyr: 0.619 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7228 proteins (3636073 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski