Beihai rhabdo-like virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Artoviridae; Peropuvirus; Beihai peropuvirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KMS9|A0A1L3KMS9_9MONO Uncharacterized protein OS=Beihai rhabdo-like virus 1 OX=1922651 PE=4 SV=1
MM1 pKa = 7.4AVDD4 pKa = 3.87IGYY7 pKa = 10.51DD8 pKa = 3.45SLNKK12 pKa = 10.12VIGSHH17 pKa = 6.6PSVNYY22 pKa = 10.68AFVSGDD28 pKa = 3.08KK29 pKa = 10.93LLAKK33 pKa = 10.2KK34 pKa = 9.59ILNMTDD40 pKa = 2.52VTTIKK45 pKa = 10.57SQLEE49 pKa = 4.0APGLVVYY56 pKa = 9.84WDD58 pKa = 4.35QIPDD62 pKa = 3.56LLNAHH67 pKa = 6.32FRR69 pKa = 11.84YY70 pKa = 10.18VDD72 pKa = 3.67GLLDD76 pKa = 3.95AQDD79 pKa = 4.66FLACFYY85 pKa = 11.46AVLDD89 pKa = 3.79KK90 pKa = 11.24QYY92 pKa = 10.93FATNYY97 pKa = 9.47KK98 pKa = 10.27SVFVVSGTPAGLLVVAQHH116 pKa = 5.95FRR118 pKa = 11.84RR119 pKa = 11.84YY120 pKa = 8.43MRR122 pKa = 11.84QCDD125 pKa = 3.91IVPPDD130 pKa = 3.72ARR132 pKa = 11.84NLFLDD137 pKa = 3.96DD138 pKa = 3.7QYY140 pKa = 11.18TIPRR144 pKa = 11.84VFPASTKK151 pKa = 9.76IVPLFKK157 pKa = 10.78VEE159 pKa = 3.81PAYY162 pKa = 10.85VHH164 pKa = 7.21SFFQDD169 pKa = 3.35SNVSFDD175 pKa = 3.95SKK177 pKa = 11.01VLSSFFNNFPSTRR190 pKa = 11.84DD191 pKa = 3.27KK192 pKa = 11.13FINKK196 pKa = 6.71YY197 pKa = 8.85TIYY200 pKa = 9.94PYY202 pKa = 10.11HH203 pKa = 7.12CPASAPTTYY212 pKa = 10.08ISSPEE217 pKa = 3.93VEE219 pKa = 4.78SILMVSVASLCTAYY233 pKa = 10.36SQRR236 pKa = 11.84SRR238 pKa = 11.84ITQQIGKK245 pKa = 9.73RR246 pKa = 11.84ITAMLAGSNYY256 pKa = 9.47PSIRR260 pKa = 11.84VDD262 pKa = 4.01SINDD266 pKa = 3.44ALTAIDD272 pKa = 4.51WKK274 pKa = 10.76PEE276 pKa = 3.92EE277 pKa = 4.15QPPAIVEE284 pKa = 4.49AIGWVCAINKK294 pKa = 9.87DD295 pKa = 4.19GIIDD299 pKa = 4.0PVTSINDD306 pKa = 3.5LMDD309 pKa = 5.07PDD311 pKa = 3.58EE312 pKa = 4.21WAARR316 pKa = 11.84YY317 pKa = 7.67HH318 pKa = 6.06VPDD321 pKa = 3.04WVKK324 pKa = 10.53FLFVQMRR331 pKa = 11.84LVYY334 pKa = 10.65SHH336 pKa = 6.88FQCSSINFALVILPLIEE353 pKa = 4.86SLTTPGTSYY362 pKa = 9.85WKK364 pKa = 10.47KK365 pKa = 10.91DD366 pKa = 2.73IDD368 pKa = 3.86ALNEE372 pKa = 3.86IKK374 pKa = 10.69NIIIDD379 pKa = 4.11CPYY382 pKa = 8.72SNCMFTIPEE391 pKa = 4.03KK392 pKa = 10.56RR393 pKa = 11.84RR394 pKa = 11.84ISAFPRR400 pKa = 11.84LVYY403 pKa = 10.27MGTHH407 pKa = 5.25YY408 pKa = 10.31HH409 pKa = 6.63AKK411 pKa = 9.98HH412 pKa = 6.34LSDD415 pKa = 4.56EE416 pKa = 4.23KK417 pKa = 11.2DD418 pKa = 3.38KK419 pKa = 11.64EE420 pKa = 4.11DD421 pKa = 3.49FKK423 pKa = 11.57FYY425 pKa = 9.19VTEE428 pKa = 4.18GTVHH432 pKa = 6.47HH433 pKa = 6.58FKK435 pKa = 10.64EE436 pKa = 4.55KK437 pKa = 10.26KK438 pKa = 9.99DD439 pKa = 3.65QEE441 pKa = 4.29LCRR444 pKa = 11.84LQVQSLPRR452 pKa = 11.84PTVSALAILGSATPAEE468 pKa = 4.23KK469 pKa = 9.82MEE471 pKa = 5.06EE472 pKa = 4.09IVSGYY477 pKa = 11.26DD478 pKa = 3.34NILQAAIYY486 pKa = 10.49QEE488 pKa = 4.18MKK490 pKa = 10.38RR491 pKa = 11.84NYY493 pKa = 8.76PQAPWVTYY501 pKa = 9.92KK502 pKa = 10.56HH503 pKa = 5.86EE504 pKa = 4.35KK505 pKa = 8.77YY506 pKa = 10.3KK507 pKa = 10.32RR508 pKa = 11.84AEE510 pKa = 4.06VARR513 pKa = 11.84MRR515 pKa = 11.84TSLLNIIDD523 pKa = 3.99QKK525 pKa = 10.65YY526 pKa = 9.64QDD528 pKa = 4.67AITYY532 pKa = 10.5LNEE535 pKa = 4.44LYY537 pKa = 10.2MGAQNDD543 pKa = 3.6EE544 pKa = 3.94TRR546 pKa = 11.84IQLKK550 pKa = 8.14TKK552 pKa = 10.31KK553 pKa = 9.87DD554 pKa = 3.61DD555 pKa = 3.59FMKK558 pKa = 10.52TVLGLKK564 pKa = 10.38AKK566 pKa = 8.92LTTLKK571 pKa = 10.76EE572 pKa = 3.82EE573 pKa = 4.12MAIPEE578 pKa = 4.04NPEE581 pKa = 3.71FEE583 pKa = 4.41VEE585 pKa = 4.01RR586 pKa = 11.84EE587 pKa = 3.97KK588 pKa = 11.35LIDD591 pKa = 3.71LAKK594 pKa = 10.41PILDD598 pKa = 4.97AINAIIMM605 pKa = 4.14

Molecular weight: 69.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KMS9|A0A1L3KMS9_9MONO Uncharacterized protein OS=Beihai rhabdo-like virus 1 OX=1922651 PE=4 SV=1
MM1 pKa = 7.38TKK3 pKa = 10.22RR4 pKa = 11.84EE5 pKa = 4.16ILSCDD10 pKa = 3.61KK11 pKa = 10.41LRR13 pKa = 11.84EE14 pKa = 4.43MISSTSVSGPLSSQGNDD31 pKa = 2.54INMLSPLDD39 pKa = 3.71QQILNEE45 pKa = 4.02LRR47 pKa = 11.84GSEE50 pKa = 4.35SLPPSTILPVRR61 pKa = 11.84RR62 pKa = 11.84SRR64 pKa = 11.84TVEE67 pKa = 3.93DD68 pKa = 3.49LTNIPTSQPIEE79 pKa = 3.84QRR81 pKa = 11.84LVFSSPIEE89 pKa = 3.93STKK92 pKa = 10.93RR93 pKa = 11.84KK94 pKa = 7.92ITEE97 pKa = 4.47DD98 pKa = 3.64NSPMSSGTDD107 pKa = 3.22QLISKK112 pKa = 8.33LTEE115 pKa = 4.06LPLRR119 pKa = 11.84PYY121 pKa = 10.29TPLTEE126 pKa = 4.74LLPISDD132 pKa = 4.4ILDD135 pKa = 3.94PQPAATSTTRR145 pKa = 11.84SSPFKK150 pKa = 10.44KK151 pKa = 10.01RR152 pKa = 11.84STRR155 pKa = 11.84ASSVHH160 pKa = 5.16TKK162 pKa = 10.01ISYY165 pKa = 10.22VPTLPSEE172 pKa = 4.23IQEE175 pKa = 4.48SEE177 pKa = 3.83TDD179 pKa = 3.06TDD181 pKa = 3.83IEE183 pKa = 4.08VDD185 pKa = 3.8DD186 pKa = 4.23AHH188 pKa = 6.74SLSVPMSRR196 pKa = 11.84FLQASTPPPPSPSTSHH212 pKa = 6.94NLNTYY217 pKa = 9.71NLASAYY223 pKa = 9.76LAQSDD228 pKa = 4.53PIEE231 pKa = 4.18PTGNYY236 pKa = 7.35TVSSKK241 pKa = 10.01MMAFLSGEE249 pKa = 4.33SKK251 pKa = 9.83TVDD254 pKa = 2.51SDD256 pKa = 3.19MDD258 pKa = 3.96LEE260 pKa = 4.41LKK262 pKa = 10.42RR263 pKa = 11.84WATDD267 pKa = 2.63RR268 pKa = 11.84WDD270 pKa = 3.64QLINPPKK277 pKa = 10.26GLKK280 pKa = 9.9CEE282 pKa = 4.21HH283 pKa = 6.65FALCLKK289 pKa = 10.16YY290 pKa = 10.67ISGNSNRR297 pKa = 11.84HH298 pKa = 4.51KK299 pKa = 10.29AYY301 pKa = 10.41HH302 pKa = 5.46IAKK305 pKa = 9.99GNFHH309 pKa = 6.37MACATEE315 pKa = 4.43QVWSDD320 pKa = 3.6LPKK323 pKa = 9.93LTKK326 pKa = 8.36TTSNLKK332 pKa = 10.16SALSTPHH339 pKa = 5.34MTIEE343 pKa = 4.14LQSLITQHH351 pKa = 5.55TQLLQDD357 pKa = 3.82NNRR360 pKa = 11.84LITTLQEE367 pKa = 4.2YY368 pKa = 10.41GGSLINQYY376 pKa = 9.62QDD378 pKa = 2.9HH379 pKa = 6.61TNQLKK384 pKa = 10.9NITQEE389 pKa = 3.85VSNTLSSKK397 pKa = 10.22ISSLVSAIEE406 pKa = 4.04KK407 pKa = 10.7LSGLSDD413 pKa = 3.58KK414 pKa = 11.07EE415 pKa = 3.79QGKK418 pKa = 9.08LLKK421 pKa = 10.18RR422 pKa = 11.84KK423 pKa = 9.93SLGTEE428 pKa = 4.02LLDD431 pKa = 4.19SSLSSVGPSASATRR445 pKa = 11.84SQSSRR450 pKa = 11.84RR451 pKa = 11.84GSTVSTYY458 pKa = 10.22PYY460 pKa = 9.38YY461 pKa = 10.52YY462 pKa = 11.04SNVTSLPP469 pKa = 3.52

Molecular weight: 51.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3247

469

2173

1082.3

122.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.928 ± 0.818

1.848 ± 0.382

5.082 ± 0.516

4.527 ± 0.381

4.558 ± 1.037

4.373 ± 0.642

3.08 ± 0.508

7.545 ± 0.595

4.958 ± 0.732

11.426 ± 0.966

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.033 ± 0.21

4.743 ± 0.244

4.774 ± 0.912

5.112 ± 0.398

4.158 ± 0.093

9.701 ± 1.744

6.19 ± 1.006

5.698 ± 0.666

1.17 ± 0.171

4.096 ± 0.404
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski