Halorhodospira halochloris (Ectothiorhodospira halochloris)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Halorhodospira

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2566 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X8XA14|A0A0X8XA14_HALHR Uncharacterized protein OS=Halorhodospira halochloris OX=1052 GN=HH1059_15450 PE=4 SV=1
MM1 pKa = 6.98PQLRR5 pKa = 11.84RR6 pKa = 11.84LYY8 pKa = 9.13PAAIALSSAVCTGSLSAAEE27 pKa = 4.56RR28 pKa = 11.84FTGGYY33 pKa = 9.36IGVEE37 pKa = 3.89GDD39 pKa = 3.85LLSSVTFEE47 pKa = 4.95RR48 pKa = 11.84DD49 pKa = 2.92FDD51 pKa = 4.33DD52 pKa = 5.04GFAGEE57 pKa = 4.29VVEE60 pKa = 6.21DD61 pKa = 4.01FVEE64 pKa = 4.7YY65 pKa = 10.6EE66 pKa = 4.31DD67 pKa = 4.04PTDD70 pKa = 3.9FLEE73 pKa = 6.08NDD75 pKa = 3.61DD76 pKa = 3.93QQEE79 pKa = 4.37GFASALMYY87 pKa = 9.75TSHH90 pKa = 6.78EE91 pKa = 4.13AGIEE95 pKa = 4.03EE96 pKa = 4.55VVPGGSVILGGGMQQDD112 pKa = 3.79GFYY115 pKa = 10.68SGIEE119 pKa = 3.77ARR121 pKa = 11.84YY122 pKa = 8.98HH123 pKa = 5.79FGGVDD128 pKa = 3.36EE129 pKa = 4.95EE130 pKa = 5.13FEE132 pKa = 4.97DD133 pKa = 4.8DD134 pKa = 4.67LEE136 pKa = 4.41EE137 pKa = 4.45SLEE140 pKa = 4.13FEE142 pKa = 4.78DD143 pKa = 6.07GYY145 pKa = 11.13SISARR150 pKa = 11.84FGTLLRR156 pKa = 11.84EE157 pKa = 4.5GNVMLYY163 pKa = 10.95GSLGYY168 pKa = 8.81ATRR171 pKa = 11.84EE172 pKa = 3.56VTYY175 pKa = 10.51EE176 pKa = 3.93VFEE179 pKa = 5.45DD180 pKa = 5.25DD181 pKa = 5.14EE182 pKa = 5.66NSDD185 pKa = 4.26SNDD188 pKa = 2.93HH189 pKa = 6.17SGFRR193 pKa = 11.84AGIGLEE199 pKa = 3.95YY200 pKa = 10.59RR201 pKa = 11.84PDD203 pKa = 3.48YY204 pKa = 10.83PPIFVRR210 pKa = 11.84LEE212 pKa = 3.74TSRR215 pKa = 11.84TDD217 pKa = 4.53FGDD220 pKa = 3.25EE221 pKa = 4.22TYY223 pKa = 11.22EE224 pKa = 5.6DD225 pKa = 4.18EE226 pKa = 6.03DD227 pKa = 5.25DD228 pKa = 4.52GNEE231 pKa = 4.31FDD233 pKa = 6.02FADD236 pKa = 3.54IDD238 pKa = 4.41DD239 pKa = 4.61LVEE242 pKa = 4.16HH243 pKa = 6.97AAHH246 pKa = 6.77LGVGYY251 pKa = 10.61RR252 pKa = 11.84FF253 pKa = 3.62

Molecular weight: 28.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z6EZW3|A0A2Z6EZW3_HALHR Uncharacterized protein OS=Halorhodospira halochloris OX=1052 GN=HH1059_19610 PE=4 SV=1
MM1 pKa = 7.72AKK3 pKa = 10.09PRR5 pKa = 11.84GNRR8 pKa = 11.84VTVTRR13 pKa = 11.84RR14 pKa = 11.84TSKK17 pKa = 9.53RR18 pKa = 11.84TQALVKK24 pKa = 10.06APHH27 pKa = 6.94ILRR30 pKa = 11.84SNKK33 pKa = 9.43GNSPLKK39 pKa = 10.01VIRR42 pKa = 11.84VALL45 pKa = 3.92

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2566

0

2566

818812

43

1910

319.1

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.48 ± 0.065

0.97 ± 0.015

6.015 ± 0.046

7.571 ± 0.054

3.232 ± 0.026

8.016 ± 0.045

2.402 ± 0.025

5.124 ± 0.035

2.861 ± 0.034

10.473 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.142 ± 0.023

2.776 ± 0.03

4.707 ± 0.033

4.429 ± 0.035

7.498 ± 0.052

5.804 ± 0.032

4.591 ± 0.03

6.903 ± 0.045

1.378 ± 0.02

2.629 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski