Paenibacillus donghaensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2K6L0|A0A2Z2K6L0_9BACL Uncharacterized protein OS=Paenibacillus donghaensis OX=414771 GN=B9T62_14210 PE=4 SV=1
MM1 pKa = 7.17SKK3 pKa = 10.13KK4 pKa = 9.97RR5 pKa = 11.84SAYY8 pKa = 8.41VLPVVVLLFLSLITTASTVGTKK30 pKa = 10.53ANAQSGVSAQQLTVYY45 pKa = 9.9IQSLTSGDD53 pKa = 3.36GSTALSADD61 pKa = 3.81EE62 pKa = 4.3IEE64 pKa = 4.59WYY66 pKa = 10.26QGAEE70 pKa = 3.85ATEE73 pKa = 4.44VFAQRR78 pKa = 11.84EE79 pKa = 4.14PEE81 pKa = 3.76AAAEE85 pKa = 4.1IGGPPDD91 pKa = 4.1DD92 pKa = 4.38YY93 pKa = 11.68YY94 pKa = 10.66IVNDD98 pKa = 3.81SDD100 pKa = 4.34SLTDD104 pKa = 3.62YY105 pKa = 10.62PVAATATVTLQIYY118 pKa = 10.04DD119 pKa = 3.46HH120 pKa = 6.93TGNIEE125 pKa = 4.18DD126 pKa = 6.3LDD128 pKa = 4.42INWNEE133 pKa = 4.0QVTLEE138 pKa = 4.06QFVKK142 pKa = 10.59LFSQNEE148 pKa = 4.18VFDD151 pKa = 5.19LSQSPYY157 pKa = 10.32HH158 pKa = 6.21ITVQDD163 pKa = 3.69GQITSFVQQYY173 pKa = 8.23TPP175 pKa = 3.45

Molecular weight: 19.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z2KEI0|A0A2Z2KEI0_9BACL ABC transporter permease OS=Paenibacillus donghaensis OX=414771 GN=B9T62_12580 PE=3 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVSKK11 pKa = 10.72RR12 pKa = 11.84KK13 pKa = 8.71KK14 pKa = 8.1VHH16 pKa = 5.42GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTKK25 pKa = 10.06NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7234

0

7234

2279734

10

4769

315.1

35.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.344 ± 0.038

0.805 ± 0.009

5.02 ± 0.021

6.793 ± 0.029

4.041 ± 0.02

7.416 ± 0.027

2.006 ± 0.014

6.559 ± 0.025

5.185 ± 0.03

10.515 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.758 ± 0.017

3.904 ± 0.022

3.988 ± 0.018

4.005 ± 0.021

4.969 ± 0.024

6.529 ± 0.026

5.392 ± 0.028

6.849 ± 0.024

1.258 ± 0.011

3.663 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski