Paenibacillus phage Wanderer

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gochnauervirinae; Wanderervirus; Paenibacillus virus Wanderer

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345ARM3|A0A345ARM3_9CAUD Uncharacterized protein OS=Paenibacillus phage Wanderer OX=2249779 GN=WANDERER_60 PE=4 SV=1
MM1 pKa = 7.85IYY3 pKa = 10.56GIIDD7 pKa = 3.64GSVLIEE13 pKa = 4.16TYY15 pKa = 7.4EE16 pKa = 4.31TPEE19 pKa = 3.84EE20 pKa = 4.13AYY22 pKa = 9.85EE23 pKa = 4.07AAKK26 pKa = 10.24VAYY29 pKa = 7.61EE30 pKa = 3.82ATGDD34 pKa = 3.76FFGVIAVANDD44 pKa = 3.33AKK46 pKa = 11.02GIADD50 pKa = 3.64NAA52 pKa = 4.12

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345ARI8|A0A345ARI8_9CAUD Uncharacterized protein OS=Paenibacillus phage Wanderer OX=2249779 GN=WANDERER_25 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84GKK4 pKa = 10.27AVRR7 pKa = 11.84KK8 pKa = 7.34EE9 pKa = 3.76LKK11 pKa = 9.77RR12 pKa = 11.84FIRR15 pKa = 11.84NGVSEE20 pKa = 4.08TLRR23 pKa = 11.84YY24 pKa = 9.33EE25 pKa = 3.88KK26 pKa = 10.92AFDD29 pKa = 2.94VWRR32 pKa = 11.84IKK34 pKa = 10.56RR35 pKa = 11.84LRR37 pKa = 11.84DD38 pKa = 3.33LLFVSS43 pKa = 4.85

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

12738

40

1233

176.9

20.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.555 ± 0.661

1.076 ± 0.165

6.1 ± 0.386

8.306 ± 0.419

3.965 ± 0.253

6.194 ± 0.299

1.743 ± 0.206

6.736 ± 0.387

8.479 ± 0.355

9.005 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.771 ± 0.166

4.695 ± 0.186

3.234 ± 0.16

3.988 ± 0.183

5.252 ± 0.395

5.323 ± 0.294

5.087 ± 0.184

6.563 ± 0.292

1.327 ± 0.15

3.603 ± 0.358
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski