Wuhan insect virus 28

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KFB1|A0A1L3KFB1_9VIRU RNA-directed RNA polymerase OS=Wuhan insect virus 28 OX=1923732 PE=4 SV=1
MM1 pKa = 7.38EE2 pKa = 6.35RR3 pKa = 11.84IINNGGNWTLQTSQNLRR20 pKa = 11.84LGEE23 pKa = 3.77QLQANNNNNNMRR35 pKa = 11.84EE36 pKa = 4.39NIHH39 pKa = 4.93HH40 pKa = 5.84TRR42 pKa = 11.84RR43 pKa = 11.84DD44 pKa = 3.24GHH46 pKa = 4.7VKK48 pKa = 10.29KK49 pKa = 10.4RR50 pKa = 11.84DD51 pKa = 3.19VGMTKK56 pKa = 10.43GLWVKK61 pKa = 10.67DD62 pKa = 3.3LTAEE66 pKa = 4.17GVEE69 pKa = 4.32PNPGPGMGASNGNGGSDD86 pKa = 3.38VFEE89 pKa = 4.37VTTSEE94 pKa = 4.07TVMDD98 pKa = 4.39RR99 pKa = 11.84FKK101 pKa = 10.78IQQQPDD107 pKa = 3.21LCGIGIINEE116 pKa = 3.86VMPINVLTPNADD128 pKa = 3.0IRR130 pKa = 11.84FGPYY134 pKa = 10.69LLDD137 pKa = 4.38HH138 pKa = 6.83EE139 pKa = 5.32LLLSEE144 pKa = 5.5DD145 pKa = 3.37LWKK148 pKa = 9.81TEE150 pKa = 3.89AADD153 pKa = 4.08EE154 pKa = 4.41GWLMSVKK161 pKa = 10.47HH162 pKa = 6.02KK163 pKa = 10.95GVVRR167 pKa = 11.84KK168 pKa = 8.33TDD170 pKa = 3.27GTEE173 pKa = 3.97AEE175 pKa = 4.04VDD177 pKa = 3.2IPLDD181 pKa = 3.82LKK183 pKa = 9.31LTCGDD188 pKa = 3.27AGLYY192 pKa = 7.94IGKK195 pKa = 8.68WAWPEE200 pKa = 3.71EE201 pKa = 4.15TSPMGALLPEE211 pKa = 4.27FQGLGTRR218 pKa = 11.84RR219 pKa = 11.84IDD221 pKa = 4.0SDD223 pKa = 3.38IKK225 pKa = 10.52NQIAAMSNFRR235 pKa = 11.84GVNLSNYY242 pKa = 8.94LAGVAPLYY250 pKa = 10.16RR251 pKa = 11.84ASLRR255 pKa = 11.84GGNHH259 pKa = 2.96VTMWLKK265 pKa = 9.41MWLDD269 pKa = 3.64FLVVDD274 pKa = 4.72LVARR278 pKa = 11.84SGANRR283 pKa = 11.84YY284 pKa = 8.88EE285 pKa = 4.51DD286 pKa = 3.31ARR288 pKa = 11.84KK289 pKa = 9.46PNVPARR295 pKa = 11.84LHH297 pKa = 6.09KK298 pKa = 10.17VSKK301 pKa = 11.24LEE303 pKa = 4.25GEE305 pKa = 4.31DD306 pKa = 3.53VNYY309 pKa = 10.69VNANMDD315 pKa = 3.41KK316 pKa = 10.69YY317 pKa = 10.68MSGNKK322 pKa = 9.22YY323 pKa = 9.61LYY325 pKa = 11.43SMGTSDD331 pKa = 5.19EE332 pKa = 4.52SMTYY336 pKa = 10.09LQYY339 pKa = 9.05TWRR342 pKa = 11.84YY343 pKa = 8.21ASPWPLNIEE352 pKa = 4.11HH353 pKa = 7.24LPTVDD358 pKa = 4.57NPDD361 pKa = 2.95GDD363 pKa = 4.27RR364 pKa = 11.84RR365 pKa = 11.84INDD368 pKa = 3.12IPIPMSINLPGADD381 pKa = 4.01EE382 pKa = 4.3FTVLLGNHH390 pKa = 6.23EE391 pKa = 4.19EE392 pKa = 4.25QILPSSPSSPSTRR405 pKa = 11.84DD406 pKa = 3.11EE407 pKa = 4.16LLGYY411 pKa = 7.8MNWYY415 pKa = 8.08MLKK418 pKa = 8.98TGSWSDD424 pKa = 3.33CMTGFEE430 pKa = 4.51LASALGVGARR440 pKa = 11.84PRR442 pKa = 11.84RR443 pKa = 11.84LNTIMNDD450 pKa = 2.99RR451 pKa = 11.84DD452 pKa = 3.99YY453 pKa = 12.08VNGLWSKK460 pKa = 11.13KK461 pKa = 9.45SIRR464 pKa = 11.84LPNTNSLPAFFEE476 pKa = 4.39PFTVQTAGANQAVDD490 pKa = 4.46LLAMRR495 pKa = 11.84PEE497 pKa = 4.45FVGPMMYY504 pKa = 10.18LYY506 pKa = 10.71NYY508 pKa = 7.26MVSASTQEE516 pKa = 3.99AAISLSMVKK525 pKa = 9.87EE526 pKa = 4.03CWIGPRR532 pKa = 11.84QGHH535 pKa = 5.0SQRR538 pKa = 11.84HH539 pKa = 4.59FQEE542 pKa = 3.39IMYY545 pKa = 9.21KK546 pKa = 9.96IPGQNRR552 pKa = 11.84FSKK555 pKa = 10.48FDD557 pKa = 3.33GMVMSNCAWMFGFSPSSTLFRR578 pKa = 11.84AITRR582 pKa = 11.84TEE584 pKa = 3.91STSAGLIKK592 pKa = 9.95MYY594 pKa = 10.39SATRR598 pKa = 11.84AGCVPALWSGFEE610 pKa = 4.06GAFFTLCMNEE620 pKa = 4.11RR621 pKa = 11.84YY622 pKa = 8.28MPPVLNGSVRR632 pKa = 11.84WPKK635 pKa = 10.52SKK637 pKa = 10.67QIXVXXSLGAPAHH650 pKa = 5.62VRR652 pKa = 11.84LARR655 pKa = 11.84TFGVFDD661 pKa = 4.42DD662 pKa = 4.94MYY664 pKa = 9.64WLNDD668 pKa = 3.61SGPMRR673 pKa = 11.84NAAYY677 pKa = 10.35YY678 pKa = 9.11YY679 pKa = 10.67AALAAEE685 pKa = 4.5RR686 pKa = 11.84EE687 pKa = 4.18PGEE690 pKa = 4.1MKK692 pKa = 10.16AARR695 pKa = 11.84WIKK698 pKa = 9.87PRR700 pKa = 11.84EE701 pKa = 4.22DD702 pKa = 3.62EE703 pKa = 4.72LPTEE707 pKa = 4.44IEE709 pKa = 5.36DD710 pKa = 4.28VPLDD714 pKa = 3.63NLATLGFDD722 pKa = 4.18WILKK726 pKa = 9.43PGQLCTYY733 pKa = 9.88CPVQNRR739 pKa = 11.84QLAWGLXFKK748 pKa = 10.31EE749 pKa = 3.95GRR751 pKa = 11.84DD752 pKa = 3.4RR753 pKa = 11.84YY754 pKa = 10.71KK755 pKa = 10.61RR756 pKa = 11.84IAMGRR761 pKa = 11.84ANEE764 pKa = 4.14TPTLSIYY771 pKa = 10.76SLNGSRR777 pKa = 11.84ALDD780 pKa = 3.52SSRR783 pKa = 11.84VIYY786 pKa = 10.21HH787 pKa = 5.86MPAVEE792 pKa = 4.96KK793 pKa = 10.2MFSYY797 pKa = 10.78LVGAQVPKK805 pKa = 10.75HH806 pKa = 5.24VVTPKK811 pKa = 10.67KK812 pKa = 9.91PVQEE816 pKa = 4.25QVDD819 pKa = 4.22VASDD823 pKa = 3.71GMPTAPGNDD832 pKa = 3.01NDD834 pKa = 5.33IEE836 pKa = 4.48FEE838 pKa = 4.24GNPIKK843 pKa = 10.54MGGKK847 pKa = 9.03NAWIGTKK854 pKa = 10.11KK855 pKa = 10.36SGRR858 pKa = 11.84SKK860 pKa = 9.76PAWMPKK866 pKa = 10.44GNDD869 pKa = 3.26DD870 pKa = 4.17RR871 pKa = 11.84DD872 pKa = 3.73ARR874 pKa = 11.84GPKK877 pKa = 9.89ANRR880 pKa = 11.84FAEE883 pKa = 4.56LDD885 pKa = 3.51SAPNGRR891 pKa = 11.84GFTNDD896 pKa = 2.85MAYY899 pKa = 8.89DD900 pKa = 3.59TGVSGGVKK908 pKa = 9.7SRR910 pKa = 11.84QPPKK914 pKa = 10.6TPDD917 pKa = 3.34DD918 pKa = 4.14DD919 pKa = 4.75AVLDD923 pKa = 3.57KK924 pKa = 10.87AIRR927 pKa = 11.84MNALAQAEE935 pKa = 4.37APPKK939 pKa = 9.16VSEE942 pKa = 4.22VMSVSEE948 pKa = 5.35ASDD951 pKa = 3.26KK952 pKa = 11.19KK953 pKa = 11.19KK954 pKa = 10.77NDD956 pKa = 3.0YY957 pKa = 10.38AQAVKK962 pKa = 10.71SGLTPSQGGNISHH975 pKa = 6.61VNHH978 pKa = 6.21QEE980 pKa = 3.93VDD982 pKa = 3.28DD983 pKa = 4.26STVMTHH989 pKa = 6.48EE990 pKa = 4.88KK991 pKa = 10.22DD992 pKa = 3.41DD993 pKa = 3.69KK994 pKa = 11.51GRR996 pKa = 11.84DD997 pKa = 3.59VVRR1000 pKa = 11.84DD1001 pKa = 3.67KK1002 pKa = 11.38PFVRR1006 pKa = 11.84QPPTLAAGLEE1016 pKa = 4.24VQKK1019 pKa = 11.05VGNKK1023 pKa = 10.19LGIFDD1028 pKa = 4.68TNVLMRR1034 pKa = 11.84DD1035 pKa = 3.48HH1036 pKa = 7.42NDD1038 pKa = 3.07QKK1040 pKa = 11.74NN1041 pKa = 3.0

Molecular weight:
115.38 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KFB1|A0A1L3KFB1_9VIRU RNA-directed RNA polymerase OS=Wuhan insect virus 28 OX=1923732 PE=4 SV=1
MM1 pKa = 7.39WSALVKK7 pKa = 10.58AYY9 pKa = 10.44GCDD12 pKa = 3.5ADD14 pKa = 4.68LSLTITVSEE23 pKa = 4.7AEE25 pKa = 4.25TIEE28 pKa = 4.16NFLISHH34 pKa = 6.9FGANKK39 pKa = 9.41SDD41 pKa = 3.41ANIRR45 pKa = 11.84SAFMKK50 pKa = 9.36WGHH53 pKa = 6.89PGTYY57 pKa = 8.77WIEE60 pKa = 4.02KK61 pKa = 9.24MSRR64 pKa = 11.84CCWGNSCRR72 pKa = 11.84QCASIVVALNPVEE85 pKa = 4.1KK86 pKa = 10.7GYY88 pKa = 10.42IAGLASTDD96 pKa = 3.17ASASFSRR103 pKa = 11.84YY104 pKa = 10.07ADD106 pKa = 3.7DD107 pKa = 4.1LTWMSDD113 pKa = 3.51CVHH116 pKa = 6.94KK117 pKa = 10.68GRR119 pKa = 11.84RR120 pKa = 11.84VLTHH124 pKa = 5.46YY125 pKa = 10.91VEE127 pKa = 5.13NNDD130 pKa = 4.15LEE132 pKa = 4.65KK133 pKa = 11.18DD134 pKa = 3.73RR135 pKa = 11.84ITGQIKK141 pKa = 9.52VQLKK145 pKa = 7.46VTLRR149 pKa = 11.84QVVMALHH156 pKa = 6.86ANCADD161 pKa = 3.44AEE163 pKa = 4.1YY164 pKa = 10.8DD165 pKa = 3.39KK166 pKa = 11.27AINRR170 pKa = 11.84ICLILGLVGDD180 pKa = 4.51KK181 pKa = 11.22GLIAVTSWALVALLSPAFEE200 pKa = 4.57FICVVLHH207 pKa = 6.47EE208 pKa = 5.02EE209 pKa = 4.38DD210 pKa = 4.14VFSKK214 pKa = 9.85TISEE218 pKa = 4.14AVEE221 pKa = 3.98TLKK224 pKa = 10.68EE225 pKa = 3.92VHH227 pKa = 6.38NNARR231 pKa = 11.84NQNRR235 pKa = 11.84WRR237 pKa = 11.84DD238 pKa = 3.21IWLSPHH244 pKa = 6.45LRR246 pKa = 11.84MHH248 pKa = 6.46LQYY251 pKa = 11.05LNEE254 pKa = 4.02VFGRR258 pKa = 11.84WSYY261 pKa = 11.21SANFDD266 pKa = 4.17AEE268 pKa = 3.9IADD271 pKa = 4.1RR272 pKa = 11.84LKK274 pKa = 10.7KK275 pKa = 10.37PKK277 pKa = 9.69IKK279 pKa = 10.23RR280 pKa = 11.84SFCMRR285 pKa = 11.84PDD287 pKa = 3.46GSVLRR292 pKa = 11.84SEE294 pKa = 4.17EE295 pKa = 4.07QYY297 pKa = 11.84ARR299 pKa = 11.84TADD302 pKa = 3.48AAIQAVVDD310 pKa = 3.47ICVGGIEE317 pKa = 4.64KK318 pKa = 10.71SGFQNLDD325 pKa = 3.2EE326 pKa = 5.06FVDD329 pKa = 4.62KK330 pKa = 10.8AYY332 pKa = 9.97EE333 pKa = 3.87WSASGSAPGTTIFYY347 pKa = 10.11EE348 pKa = 4.07VDD350 pKa = 3.08GKK352 pKa = 10.13RR353 pKa = 11.84VRR355 pKa = 11.84ISPTKK360 pKa = 9.71KK361 pKa = 9.3AWLEE365 pKa = 4.01GVPKK369 pKa = 10.05TGARR373 pKa = 11.84EE374 pKa = 3.88WMRR377 pKa = 11.84ATEE380 pKa = 3.91PRR382 pKa = 11.84VLSTLVPKK390 pKa = 10.53FEE392 pKa = 4.39NGKK395 pKa = 9.23VRR397 pKa = 11.84ALSNTEE403 pKa = 2.99NWEE406 pKa = 4.22YY407 pKa = 11.41VLEE410 pKa = 4.51SYY412 pKa = 10.92LLRR415 pKa = 11.84GFEE418 pKa = 4.1DD419 pKa = 3.48TLQEE423 pKa = 4.28VEE425 pKa = 4.13GALMKK430 pKa = 10.69EE431 pKa = 4.32GGLSEE436 pKa = 4.09VAKK439 pKa = 10.45IKK441 pKa = 10.04TRR443 pKa = 11.84MDD445 pKa = 3.29QLEE448 pKa = 4.1GRR450 pKa = 11.84FGWSYY455 pKa = 11.18DD456 pKa = 3.47YY457 pKa = 11.54KK458 pKa = 11.02SFEE461 pKa = 4.45NNHH464 pKa = 5.81TFLDD468 pKa = 4.01MSNVWNKK475 pKa = 9.47VSATLAARR483 pKa = 11.84AQDD486 pKa = 3.45AGRR489 pKa = 11.84GDD491 pKa = 3.64VASDD495 pKa = 3.26TRR497 pKa = 11.84EE498 pKa = 3.88VGQWLAKK505 pKa = 10.39AFNNVQLRR513 pKa = 11.84QMGTDD518 pKa = 3.38MPYY521 pKa = 10.46HH522 pKa = 5.84KK523 pKa = 9.37TNFGLPTGTRR533 pKa = 11.84GTAFMNTLLNKK544 pKa = 9.2VYY546 pKa = 9.3CTLAEE551 pKa = 4.2TFLRR555 pKa = 11.84TGMDD559 pKa = 3.29VNALGWGHH567 pKa = 6.5MGDD570 pKa = 4.25DD571 pKa = 4.42VIAATDD577 pKa = 3.85DD578 pKa = 4.02YY579 pKa = 10.92VTANALTMIMSNMGLMTNDD598 pKa = 3.36LKK600 pKa = 10.58TVVWYY605 pKa = 10.13EE606 pKa = 3.72GGEE609 pKa = 4.24LLRR612 pKa = 11.84KK613 pKa = 9.21WYY615 pKa = 8.14TAGVGVKK622 pKa = 10.44GYY624 pKa = 10.3LNRR627 pKa = 11.84MLPNVCCGEE636 pKa = 4.12WDD638 pKa = 3.75LKK640 pKa = 11.0AASLPDD646 pKa = 3.84DD647 pKa = 3.73KK648 pKa = 11.55VGAFNTVMTKK658 pKa = 9.09MLSRR662 pKa = 11.84GAISNYY668 pKa = 9.84AIPIMEE674 pKa = 4.75AVRR677 pKa = 11.84VGTGSTKK684 pKa = 10.4RR685 pKa = 11.84HH686 pKa = 4.84GKK688 pKa = 9.74QYY690 pKa = 11.21ALPKK694 pKa = 8.94GWLYY698 pKa = 11.35SSTVNGGAGNSLMQWFGRR716 pKa = 11.84DD717 pKa = 3.68DD718 pKa = 3.64QSVWTTNTNGLKK730 pKa = 10.63LEE732 pKa = 4.36DD733 pKa = 3.88VSRR736 pKa = 11.84EE737 pKa = 3.96MLRR740 pKa = 11.84QQPGLRR746 pKa = 11.84SLRR749 pKa = 11.84YY750 pKa = 9.68CDD752 pKa = 3.39DD753 pKa = 4.1LKK755 pKa = 11.05RR756 pKa = 11.84LEE758 pKa = 4.86IDD760 pKa = 4.34DD761 pKa = 4.57PDD763 pKa = 3.76TRR765 pKa = 11.84VRR767 pKa = 11.84LALQEE772 pKa = 4.15KK773 pKa = 8.13QASYY777 pKa = 11.27EE778 pKa = 3.76KK779 pKa = 9.51TLPKK783 pKa = 10.19KK784 pKa = 10.8VVDD787 pKa = 3.14IWFSEE792 pKa = 3.89QLLPTEE798 pKa = 4.03RR799 pKa = 11.84FGLKK803 pKa = 9.63RR804 pKa = 11.84HH805 pKa = 5.15AQKK808 pKa = 10.93VDD810 pKa = 2.92TATMVRR816 pKa = 11.84LGNRR820 pKa = 11.84LLGDD824 pKa = 3.23WLDD827 pKa = 3.48NGGRR831 pKa = 11.84VLLQDD836 pKa = 3.42VDD838 pKa = 3.75NWTHH842 pKa = 7.13LATKK846 pKa = 9.85IKK848 pKa = 9.71NAHH851 pKa = 5.02VVPLRR856 pKa = 11.84LQPNKK861 pKa = 10.1LAAIKK866 pKa = 9.85EE867 pKa = 4.34AIGYY871 pKa = 9.7RR872 pKa = 11.84GIKK875 pKa = 10.11SSTKK879 pKa = 9.11ACEE882 pKa = 4.0KK883 pKa = 10.51LIHH886 pKa = 6.8LLMAQNTNGGSATVKK901 pKa = 10.84AMVLAYY907 pKa = 10.34GMEE910 pKa = 4.18TGMSIMMQDD919 pKa = 3.15VKK921 pKa = 10.68EE922 pKa = 4.25DD923 pKa = 3.93QISHH927 pKa = 7.22PGGEE931 pKa = 4.03DD932 pKa = 3.57AMWAFLDD939 pKa = 3.75XVRR942 pKa = 11.84MMVLYY947 pKa = 9.41WHH949 pKa = 7.09GPYY952 pKa = 9.69DD953 pKa = 3.48AKK955 pKa = 10.74EE956 pKa = 3.91RR957 pKa = 11.84YY958 pKa = 8.32EE959 pKa = 4.19VYY961 pKa = 10.43SAGLAACLNKK971 pKa = 10.58NYY973 pKa = 10.49GDD975 pKa = 3.73EE976 pKa = 4.16RR977 pKa = 4.96

Molecular weight:
109.46 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

2018

977

1041

1009.0

112.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.127 ± 0.444

1.288 ± 0.297

6.244 ± 0.135

5.55 ± 0.187

2.874 ± 0.073

7.978 ± 0.198

1.833 ± 0.073

3.915 ± 0.017

5.847 ± 0.26

8.672 ± 0.556

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.063 ± 0.249

5.748 ± 0.481

4.509 ± 1.281

3.072 ± 0.068

5.798 ± 0.024

6.194 ± 0.236

5.5 ± 0.354

6.789 ± 0.314

2.527 ± 0.222

3.221 ± 0.103

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski