Salmonella phage vB_SenS_PVP-SE2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Jerseyvirus; unclassified Jerseyvirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223G0H8|A0A223G0H8_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS_PVP-SE2 OX=2024214 GN=PVPSE2_53 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.37VYY4 pKa = 10.33IISGWYY10 pKa = 9.45YY11 pKa = 10.91YY12 pKa = 10.89DD13 pKa = 3.66GADD16 pKa = 3.04ILGVYY21 pKa = 10.51LSEE24 pKa = 4.43EE25 pKa = 4.09KK26 pKa = 11.09AEE28 pKa = 4.11AAAEE32 pKa = 4.05LEE34 pKa = 4.35RR35 pKa = 11.84AKK37 pKa = 10.93GDD39 pKa = 3.55YY40 pKa = 10.88DD41 pKa = 3.62GVEE44 pKa = 3.83VRR46 pKa = 11.84EE47 pKa = 4.43WEE49 pKa = 4.24VQEE52 pKa = 4.12

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223G0I3|A0A223G0I3_9CAUD Holin class I OS=Salmonella phage vB_SenS_PVP-SE2 OX=2024214 GN=PVPSE2_08 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.34PNDD5 pKa = 3.45LVTWTGRR12 pKa = 11.84NGGTRR17 pKa = 11.84HH18 pKa = 5.7GKK20 pKa = 7.91VTSLHH25 pKa = 6.12GIYY28 pKa = 10.5ARR30 pKa = 11.84VEE32 pKa = 3.68RR33 pKa = 11.84WRR35 pKa = 11.84AHH37 pKa = 5.04AKK39 pKa = 9.08KK40 pKa = 9.8PRR42 pKa = 11.84YY43 pKa = 9.58FLMCQDD49 pKa = 3.65KK50 pKa = 11.16LIVKK54 pKa = 9.62

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12982

35

1032

216.4

23.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.66 ± 0.579

0.971 ± 0.162

6.078 ± 0.18

6.609 ± 0.432

3.798 ± 0.166

7.795 ± 0.332

1.641 ± 0.196

4.853 ± 0.177

5.939 ± 0.357

7.726 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.152

4.375 ± 0.287

3.898 ± 0.223

3.682 ± 0.231

5.716 ± 0.251

5.978 ± 0.249

6.47 ± 0.326

7.41 ± 0.327

1.448 ± 0.162

3.32 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski