Pectinobacterium phage vB_PcaM_CBB

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Mimasvirus; Pectinobacterium virus CBB

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 555 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L2CUG0|A0A1L2CUG0_9CAUD Uncharacterized protein OS=Pectinobacterium phage vB_PcaM_CBB OX=2772511 GN=CBB_98 PE=4 SV=1
MM1 pKa = 7.35IVAIYY6 pKa = 9.31IQSEE10 pKa = 4.33SCDD13 pKa = 3.26KK14 pKa = 11.01YY15 pKa = 11.54LEE17 pKa = 4.42LFVNEE22 pKa = 4.24TVHH25 pKa = 6.84EE26 pKa = 4.43IKK28 pKa = 10.64EE29 pKa = 3.94KK30 pKa = 10.78LYY32 pKa = 11.15DD33 pKa = 3.58NMEE36 pKa = 4.1MYY38 pKa = 10.58KK39 pKa = 9.93PVCEE43 pKa = 3.98YY44 pKa = 11.23DD45 pKa = 3.77VVFDD49 pKa = 4.67ASTTEE54 pKa = 4.0KK55 pKa = 10.77EE56 pKa = 4.12KK57 pKa = 11.25LDD59 pKa = 3.64VGEE62 pKa = 5.47MLSEE66 pKa = 4.17WYY68 pKa = 9.91DD69 pKa = 3.71DD70 pKa = 3.09SWNRR74 pKa = 11.84DD75 pKa = 3.53DD76 pKa = 6.7DD77 pKa = 4.34EE78 pKa = 5.27EE79 pKa = 6.5DD80 pKa = 3.71YY81 pKa = 11.77

Molecular weight: 9.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L2CVF6|A0A1L2CVF6_9CAUD Putative membrane protein OS=Pectinobacterium phage vB_PcaM_CBB OX=2772511 GN=CBB_448 PE=4 SV=1
MM1 pKa = 8.06WIRR4 pKa = 11.84TTSFFSHH11 pKa = 6.63KK12 pKa = 9.9KK13 pKa = 9.26YY14 pKa = 10.67KK15 pKa = 10.23ILFAVRR21 pKa = 11.84FRR23 pKa = 11.84VANTFIIPQIFDD35 pKa = 3.44SFQKK39 pKa = 10.48FSKK42 pKa = 9.96LKK44 pKa = 10.76SGISCLSSTMLPLKK58 pKa = 10.2IRR60 pKa = 11.84QNRR63 pKa = 11.84GWGFEE68 pKa = 3.88PPSFCFHH75 pKa = 6.08QKK77 pKa = 10.73LIIFAVRR84 pKa = 11.84FRR86 pKa = 11.84CLMYY90 pKa = 10.11YY91 pKa = 10.15IIPQKK96 pKa = 10.4FLSFHH101 pKa = 6.52LFFIKK106 pKa = 10.64YY107 pKa = 9.36

Molecular weight: 12.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

555

0

555

107545

38

3340

193.8

22.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.796 ± 0.157

1.13 ± 0.052

6.618 ± 0.104

7.31 ± 0.171

4.552 ± 0.081

5.796 ± 0.133

1.961 ± 0.071

6.847 ± 0.09

6.549 ± 0.165

7.697 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.853 ± 0.067

6.126 ± 0.128

3.259 ± 0.07

3.582 ± 0.081

4.068 ± 0.081

6.6 ± 0.121

6.379 ± 0.223

6.82 ± 0.114

1.199 ± 0.047

4.859 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski