Gordonia phage Boneham

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Montyvirus; unclassified Montyvirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L1IWH9|A0A2L1IWH9_9CAUD Uncharacterized protein OS=Gordonia phage Boneham OX=2079414 GN=SEA_BONEHAM_18 PE=4 SV=1
MM1 pKa = 7.3IVSGIAVMDD10 pKa = 4.48ASYY13 pKa = 11.28GPIIIPLGSALVGSSHH29 pKa = 7.43PEE31 pKa = 3.33FSIQFEE37 pKa = 4.24YY38 pKa = 10.78DD39 pKa = 3.22YY40 pKa = 11.51GPFINPDD47 pKa = 2.92APAFEE52 pKa = 4.58FLADD56 pKa = 3.85RR57 pKa = 11.84LEE59 pKa = 4.12VDD61 pKa = 3.49GTITRR66 pKa = 11.84NLEE69 pKa = 4.28CISYY73 pKa = 8.69PGRR76 pKa = 11.84YY77 pKa = 6.48TVEE80 pKa = 4.14KK81 pKa = 10.66VV82 pKa = 2.8

Molecular weight: 8.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L1IWG8|A0A2L1IWG8_9CAUD Major tail protein OS=Gordonia phage Boneham OX=2079414 GN=SEA_BONEHAM_23 PE=4 SV=1
MM1 pKa = 7.87PNRR4 pKa = 11.84RR5 pKa = 11.84DD6 pKa = 3.18KK7 pKa = 11.28FRR9 pKa = 11.84GNRR12 pKa = 11.84IEE14 pKa = 3.73IRR16 pKa = 11.84VRR18 pKa = 11.84AQRR21 pKa = 11.84DD22 pKa = 2.97LDD24 pKa = 3.67GFVIHH29 pKa = 7.21CYY31 pKa = 10.11DD32 pKa = 3.32NRR34 pKa = 11.84GMLVATGCYY43 pKa = 9.13HH44 pKa = 7.1KK45 pKa = 11.33SNVTMTATRR54 pKa = 11.84AASLALGVPEE64 pKa = 4.04GRR66 pKa = 11.84IKK68 pKa = 10.52ISRR71 pKa = 11.84LEE73 pKa = 3.96YY74 pKa = 10.66AA75 pKa = 4.4

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

0

109

24291

33

3319

222.9

24.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.617 ± 1.011

1.029 ± 0.143

6.163 ± 0.312

6.743 ± 0.381

3.479 ± 0.105

8.015 ± 0.292

1.721 ± 0.182

5.348 ± 0.173

5.286 ± 0.21

7.283 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.75 ± 0.164

4.1 ± 0.128

5.352 ± 0.368

3.874 ± 0.205

5.409 ± 0.296

6.043 ± 0.205

6.113 ± 0.216

6.805 ± 0.16

1.766 ± 0.11

3.104 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski