Rhodococcus phage NiceHouse

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5JHU6|A0A7D5JHU6_9CAUD Uncharacterized protein OS=Rhodococcus phage NiceHouse OX=2743998 GN=278 PE=4 SV=1
MM1 pKa = 7.46SFITAEE7 pKa = 4.15EE8 pKa = 3.99LDD10 pKa = 3.67KK11 pKa = 11.01QLRR14 pKa = 11.84EE15 pKa = 4.59HH16 pKa = 7.45ILATWGNDD24 pKa = 3.36YY25 pKa = 11.35EE26 pKa = 5.19DD27 pKa = 5.8DD28 pKa = 4.09EE29 pKa = 5.99DD30 pKa = 5.34VDD32 pKa = 4.53LSDD35 pKa = 3.37IAEE38 pKa = 4.43IFQEE42 pKa = 4.05TDD44 pKa = 3.21EE45 pKa = 4.65LVLPAGTFEE54 pKa = 4.29TVLVEE59 pKa = 4.15GGEE62 pKa = 4.66GCGDD66 pKa = 3.83EE67 pKa = 4.67YY68 pKa = 11.7YY69 pKa = 10.99VIARR73 pKa = 11.84IKK75 pKa = 9.71EE76 pKa = 4.07TGQLFRR82 pKa = 11.84LSGYY86 pKa = 8.11YY87 pKa = 10.28SSWSGVDD94 pKa = 2.87WSEE97 pKa = 5.12DD98 pKa = 3.44IEE100 pKa = 4.55HH101 pKa = 6.01VEE103 pKa = 4.14AYY105 pKa = 9.22EE106 pKa = 4.14EE107 pKa = 4.16PVVFYY112 pKa = 11.07RR113 pKa = 11.84PVV115 pKa = 2.66

Molecular weight: 13.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D5KAP3|A0A7D5KAP3_9CAUD Uncharacterized protein OS=Rhodococcus phage NiceHouse OX=2743998 GN=159 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 9.72MLGRR6 pKa = 11.84FTHH9 pKa = 5.56GHH11 pKa = 4.9CTEE14 pKa = 5.06FGCCEE19 pKa = 3.55PHH21 pKa = 5.09KK22 pKa = 9.48TVRR25 pKa = 11.84EE26 pKa = 4.12SKK28 pKa = 10.1RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84KK32 pKa = 7.03WHH34 pKa = 6.0RR35 pKa = 11.84KK36 pKa = 4.63VHH38 pKa = 5.82RR39 pKa = 11.84SEE41 pKa = 3.51VRR43 pKa = 11.84RR44 pKa = 11.84YY45 pKa = 9.16KK46 pKa = 10.6RR47 pKa = 11.84KK48 pKa = 10.18EE49 pKa = 3.38KK50 pKa = 10.83DD51 pKa = 2.45RR52 pKa = 11.84WKK54 pKa = 10.73QLAEE58 pKa = 4.24SNQLL62 pKa = 3.27

Molecular weight: 7.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

250

0

250

41644

29

2311

166.6

18.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.72 ± 0.3

0.97 ± 0.09

6.755 ± 0.161

7.014 ± 0.242

4.037 ± 0.117

6.829 ± 0.242

1.914 ± 0.112

6.119 ± 0.15

5.78 ± 0.22

7.675 ± 0.162

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.131

5.158 ± 0.174

3.926 ± 0.145

3.753 ± 0.201

4.903 ± 0.128

6.21 ± 0.173

6.198 ± 0.234

6.685 ± 0.215

1.784 ± 0.086

3.953 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski