Escherichia phage K1H

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; Escherichia virus K1H

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2XJC2|D2XJC2_9CAUD Putative spanin OS=Escherichia phage K1H OX=698487 PE=4 SV=1
MM1 pKa = 7.26MGFFKK6 pKa = 10.59VTDD9 pKa = 3.59VPSRR13 pKa = 11.84RR14 pKa = 11.84VVQYY18 pKa = 11.2ARR20 pKa = 11.84VSGSGEE26 pKa = 3.67NVVFIDD32 pKa = 4.36DD33 pKa = 4.08EE34 pKa = 4.94SVLGTPEE41 pKa = 4.9DD42 pKa = 4.3DD43 pKa = 4.1MPFADD48 pKa = 3.92KK49 pKa = 10.8TGIALPAAGMLYY61 pKa = 9.45EE62 pKa = 4.44IPYY65 pKa = 10.0LADD68 pKa = 3.94AGDD71 pKa = 4.2VYY73 pKa = 10.94FSVQPKK79 pKa = 9.02DD80 pKa = 3.4TEE82 pKa = 4.33LVDD85 pKa = 4.4GSATITVEE93 pKa = 4.19VKK95 pKa = 10.37AGKK98 pKa = 9.82APCVLTWYY106 pKa = 10.52KK107 pKa = 10.74DD108 pKa = 3.15GKK110 pKa = 10.02EE111 pKa = 4.02VVNAPEE117 pKa = 4.14EE118 pKa = 4.26ALSLTVNAVGEE129 pKa = 4.39YY130 pKa = 9.43FVKK133 pKa = 9.89VTDD136 pKa = 3.88ADD138 pKa = 3.75GVEE141 pKa = 4.36AVSKK145 pKa = 9.91AAKK148 pKa = 7.66VTKK151 pKa = 10.46PEE153 pKa = 3.79

Molecular weight: 16.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2XJF0|D2XJF0_9CAUD Uncharacterized protein OS=Escherichia phage K1H OX=698487 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 9.71EE3 pKa = 4.14ALPKK7 pKa = 10.57LSQKK11 pKa = 9.0TLTGSPSVIFSRR23 pKa = 11.84VSAAGLSRR31 pKa = 11.84SDD33 pKa = 3.17LRR35 pKa = 11.84EE36 pKa = 3.59FRR38 pKa = 11.84KK39 pKa = 9.09MRR41 pKa = 11.84LSGRR45 pKa = 11.84EE46 pKa = 3.5ARR48 pKa = 11.84LANRR52 pKa = 11.84LARR55 pKa = 11.84RR56 pKa = 11.84EE57 pKa = 4.31SNSDD61 pKa = 2.53SSTRR65 pKa = 11.84DD66 pKa = 2.99TSRR69 pKa = 11.84RR70 pKa = 11.84SSSTSSASAALQSSLGSRR88 pKa = 11.84LRR90 pKa = 11.84QQLQNRR96 pKa = 11.84GCAIYY101 pKa = 10.86KK102 pKa = 7.48LTWKK106 pKa = 10.51EE107 pKa = 3.58KK108 pKa = 6.33TTPSGLLYY116 pKa = 10.41SQLVASAHH124 pKa = 4.65RR125 pKa = 11.84TKK127 pKa = 11.12EE128 pKa = 3.91NDD130 pKa = 3.02SSLARR135 pKa = 11.84TNWPTPTANDD145 pKa = 3.34FRR147 pKa = 11.84GSGEE151 pKa = 4.22TVIRR155 pKa = 11.84TDD157 pKa = 3.09GRR159 pKa = 11.84DD160 pKa = 3.1RR161 pKa = 11.84TFDD164 pKa = 3.44RR165 pKa = 11.84LDD167 pKa = 3.27YY168 pKa = 10.57STEE171 pKa = 3.89QGLKK175 pKa = 10.12ASQPTRR181 pKa = 11.84TTASGQMLTGSDD193 pKa = 3.48AVMEE197 pKa = 4.3SSGRR201 pKa = 11.84LNPAHH206 pKa = 6.53SRR208 pKa = 11.84WLMGYY213 pKa = 8.97PPEE216 pKa = 4.44WDD218 pKa = 3.61DD219 pKa = 4.4CAVTAMPSSRR229 pKa = 11.84KK230 pKa = 9.61

Molecular weight: 25.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12457

55

984

249.1

27.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.026 ± 0.568

1.044 ± 0.15

6.334 ± 0.232

6.599 ± 0.421

3.661 ± 0.152

7.707 ± 0.246

1.654 ± 0.201

5.122 ± 0.165

5.868 ± 0.381

7.771 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.148

4.086 ± 0.241

4.014 ± 0.215

3.869 ± 0.379

5.467 ± 0.297

6.318 ± 0.377

6.462 ± 0.365

6.944 ± 0.329

1.405 ± 0.172

3.307 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski