Desulfosporosinus acidiphilus (strain DSM 22704 / JCM 16185 / SJ4)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfosporosinus; Desulfosporosinus acidiphilus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4469 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I4DAC0|I4DAC0_DESAJ Putative aminopeptidase OS=Desulfosporosinus acidiphilus (strain DSM 22704 / JCM 16185 / SJ4) OX=646529 GN=Desaci_3863 PE=4 SV=1
MM1 pKa = 7.29SVSSTSTSTALTGLSGYY18 pKa = 10.18DD19 pKa = 3.25FSGVISAMMQNYY31 pKa = 8.92DD32 pKa = 3.7LPEE35 pKa = 4.15TQMQTQEE42 pKa = 4.17STLQSQQSAWQAINTSLSALNDD64 pKa = 4.02TITALQSADD73 pKa = 2.81TWDD76 pKa = 3.44ATTATSSNTSAVTATGGSGAMAGNYY101 pKa = 7.07TVNVTQTAKK110 pKa = 10.95AEE112 pKa = 4.13NVVTKK117 pKa = 10.42VMQNTNLSSNNWSFQIGVGTNPLQNVTISSQTPTLTDD154 pKa = 2.82IVNAINSAQAGVTATLIQIDD174 pKa = 4.1SNNDD178 pKa = 2.96YY179 pKa = 10.51RR180 pKa = 11.84ISITANQTGANNYY193 pKa = 9.39VKK195 pKa = 10.73FSDD198 pKa = 3.76PNGAISNLGITLDD211 pKa = 3.64STTGQVAASDD221 pKa = 4.35DD222 pKa = 3.86FTGNLSAMTSAQGGITQDD240 pKa = 3.31SQDD243 pKa = 3.97ASFTMNGISISSASNTVTSAIQGVTLNLLAGGSSTVSVAADD284 pKa = 3.05SSVAQKK290 pKa = 10.72AVQSFVDD297 pKa = 5.0AYY299 pKa = 11.49NSTQSLIASDD309 pKa = 4.79LNYY312 pKa = 9.4DD313 pKa = 3.9TTTKK317 pKa = 10.01TAGALFGDD325 pKa = 4.35AQLEE329 pKa = 4.61DD330 pKa = 3.32IQSRR334 pKa = 11.84LRR336 pKa = 11.84QMMGSTFLNQTSPDD350 pKa = 3.7NLLSTVGISTSSDD363 pKa = 2.96NFGEE367 pKa = 4.5SAALTFDD374 pKa = 3.36TSKK377 pKa = 10.58FAQAYY382 pKa = 7.28AANPQSVANLFSAPYY397 pKa = 10.35NGVTPSNGLGGTAIQGLGNTLSAYY421 pKa = 10.27LNPLIEE427 pKa = 4.36YY428 pKa = 10.14GGTLSEE434 pKa = 4.68INTNYY439 pKa = 10.53SNQISNLEE447 pKa = 4.09TQISDD452 pKa = 3.65FQEE455 pKa = 3.77QSTNYY460 pKa = 10.32QNMLNAKK467 pKa = 7.5FTNLEE472 pKa = 4.27TILSKK477 pKa = 11.08LNSQGTWLTNQINSLPSYY495 pKa = 10.21SSSSSSKK502 pKa = 10.71

Molecular weight: 52.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I4D002|I4D002_DESAJ Uncharacterized protein OS=Desulfosporosinus acidiphilus (strain DSM 22704 / JCM 16185 / SJ4) OX=646529 GN=Desaci_0005 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.19QPKK8 pKa = 8.78NRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.44KK13 pKa = 10.09RR14 pKa = 11.84VHH16 pKa = 5.93GFLSRR21 pKa = 11.84MSTPTGRR28 pKa = 11.84NVIKK32 pKa = 10.5RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.52GRR39 pKa = 11.84KK40 pKa = 8.8KK41 pKa = 10.81LSVV44 pKa = 3.15

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4469

0

4469

1329414

29

2862

297.5

33.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.588 ± 0.042

1.094 ± 0.015

4.785 ± 0.026

6.611 ± 0.044

4.129 ± 0.03

7.431 ± 0.038

1.848 ± 0.016

7.499 ± 0.038

6.046 ± 0.031

10.484 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.595 ± 0.019

4.327 ± 0.032

3.973 ± 0.025

3.875 ± 0.029

4.636 ± 0.033

6.353 ± 0.037

5.405 ± 0.042

6.987 ± 0.031

1.093 ± 0.017

3.241 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski