Burkholderia phage JG068

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Okabevirinae; Mguuvirus; Burkholderia virus JG068

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3PCI7|U3PCI7_9CAUD Uncharacterized protein OS=Burkholderia phage JG068 OX=1401297 GN=JG068_011 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84RR3 pKa = 11.84DD4 pKa = 3.08EE5 pKa = 4.26SAFEE9 pKa = 3.9KK10 pKa = 10.85VINNTFMPSLVDD22 pKa = 5.44GQDD25 pKa = 2.17AWYY28 pKa = 9.69EE29 pKa = 3.9LRR31 pKa = 11.84PVSDD35 pKa = 4.02FALPEE40 pKa = 4.44GDD42 pKa = 5.37LPDD45 pKa = 5.05AYY47 pKa = 9.6MVQIGYY53 pKa = 9.64DD54 pKa = 3.93LPCNDD59 pKa = 4.37GGPTRR64 pKa = 11.84VQWGRR69 pKa = 11.84WFVIPSEE76 pKa = 4.62ADD78 pKa = 2.95DD79 pKa = 3.73ATIVKK84 pKa = 9.18TMFDD88 pKa = 3.45AYY90 pKa = 10.86SAFWLHH96 pKa = 6.13EE97 pKa = 3.69MRR99 pKa = 11.84EE100 pKa = 4.1VFKK103 pKa = 11.66YY104 pKa = 10.35EE105 pKa = 3.83GARR108 pKa = 11.84VLNPHH113 pKa = 7.26RR114 pKa = 11.84YY115 pKa = 9.55LINLNEE121 pKa = 4.11AAA123 pKa = 4.84

Molecular weight: 14.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3PFN3|U3PFN3_9CAUD Uncharacterized protein OS=Burkholderia phage JG068 OX=1401297 GN=JG068_07 PE=4 SV=1
MM1 pKa = 6.91STVVYY6 pKa = 10.65QFDD9 pKa = 4.08HH10 pKa = 6.47KK11 pKa = 11.01AEE13 pKa = 4.6HH14 pKa = 6.56YY15 pKa = 10.35GLPQPAKK22 pKa = 8.85TAINTHH28 pKa = 4.99VKK30 pKa = 10.61GKK32 pKa = 8.88ATQDD36 pKa = 2.44RR37 pKa = 11.84WKK39 pKa = 10.75ARR41 pKa = 11.84RR42 pKa = 11.84EE43 pKa = 4.11ANTARR48 pKa = 11.84PSNQRR53 pKa = 11.84GG54 pKa = 3.2

Molecular weight: 6.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

13050

36

1345

266.3

29.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.801 ± 0.711

0.72 ± 0.133

6.061 ± 0.189

5.747 ± 0.386

3.586 ± 0.232

8.092 ± 0.364

1.923 ± 0.221

4.659 ± 0.22

5.165 ± 0.307

7.716 ± 0.357

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.736 ± 0.201

4.199 ± 0.3

4.291 ± 0.254

5.004 ± 0.385

6.215 ± 0.28

5.51 ± 0.285

5.839 ± 0.313

6.138 ± 0.282

1.556 ± 0.137

3.042 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski