Streptomyces sp. CB02923

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7549 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q4VJU3|A0A1Q4VJU3_9ACTN MarR family transcriptional regulator OS=Streptomyces sp. CB02923 OX=1718985 GN=A6A06_28080 PE=4 SV=1
MM1 pKa = 7.55LTTQYY6 pKa = 11.12VPGAPNWLDD15 pKa = 3.32LGVPDD20 pKa = 4.79TEE22 pKa = 4.44AAAAFYY28 pKa = 10.74GGVFGWTFRR37 pKa = 11.84SAGPDD42 pKa = 2.8AGGYY46 pKa = 10.84GFFQLDD52 pKa = 3.64GKK54 pKa = 8.24TVAGIGPLTEE64 pKa = 4.37EE65 pKa = 4.53GAGASWTVHH74 pKa = 4.73FHH76 pKa = 6.54SPDD79 pKa = 2.91VDD81 pKa = 3.53ALAKK85 pKa = 9.7TVEE88 pKa = 4.29QAGGTLRR95 pKa = 11.84FGPCDD100 pKa = 3.19VFTAGRR106 pKa = 11.84LAGFTDD112 pKa = 3.67PAGAEE117 pKa = 3.96FAVWQPRR124 pKa = 11.84DD125 pKa = 3.69NQGLEE130 pKa = 4.23VAGSTNALCWTEE142 pKa = 5.56LYY144 pKa = 7.53TTDD147 pKa = 3.1VAAAKK152 pKa = 10.34DD153 pKa = 4.09FYY155 pKa = 10.59RR156 pKa = 11.84TVLGWEE162 pKa = 4.25TVDD165 pKa = 4.04NDD167 pKa = 3.19MGEE170 pKa = 4.08NMIYY174 pKa = 9.64TVASPAGTDD183 pKa = 3.38PAGDD187 pKa = 3.72SGHH190 pKa = 6.92AGIVQLSPDD199 pKa = 3.59HH200 pKa = 6.51LAAGSTSEE208 pKa = 3.45WHH210 pKa = 6.95PYY212 pKa = 9.92FGVADD217 pKa = 4.17CDD219 pKa = 3.51ATTAASTEE227 pKa = 4.01RR228 pKa = 11.84GATLVIPPTDD238 pKa = 3.19IEE240 pKa = 4.64GVGRR244 pKa = 11.84VAMLTDD250 pKa = 4.16PFGAPYY256 pKa = 11.08ALITPSPMM264 pKa = 3.56

Molecular weight: 27.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q4VQ44|A0A1Q4VQ44_9ACTN Uncharacterized protein OS=Streptomyces sp. CB02923 OX=1718985 GN=A6A06_23090 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7549

0

7549

2594322

29

9489

343.7

36.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.025 ± 0.052

0.833 ± 0.008

5.975 ± 0.025

5.527 ± 0.03

2.727 ± 0.016

9.683 ± 0.029

2.399 ± 0.014

2.937 ± 0.022

2.083 ± 0.025

10.327 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.704 ± 0.012

1.682 ± 0.016

6.29 ± 0.028

2.817 ± 0.019

8.272 ± 0.031

4.897 ± 0.021

6.072 ± 0.021

8.219 ± 0.024

1.475 ± 0.012

2.056 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski