Pseudochrobactrum asaccharolyticum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Brucellaceae; Pseudochrobactrum

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4256 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A366DNR6|A0A366DNR6_9RHIZ Putative polyketide hydroxylase OS=Pseudochrobactrum asaccharolyticum OX=354351 GN=DFR47_11129 PE=4 SV=1
MM1 pKa = 7.1KK2 pKa = 9.93TILTAIALLSATTFASAEE20 pKa = 3.86THH22 pKa = 5.01YY23 pKa = 11.18VIVAFDD29 pKa = 3.79RR30 pKa = 11.84NMTEE34 pKa = 3.73SSVIEE39 pKa = 4.24TFKK42 pKa = 11.33DD43 pKa = 3.11LDD45 pKa = 3.91DD46 pKa = 5.52FEE48 pKa = 5.37TCFDD52 pKa = 4.22LAVSSGEE59 pKa = 4.31GEE61 pKa = 4.02WTSCVPVTGAAEE73 pKa = 4.37AYY75 pKa = 9.9VLSLPEE81 pKa = 4.52DD82 pKa = 3.8SEE84 pKa = 4.56ACRR87 pKa = 11.84LAAEE91 pKa = 5.05FPTDD95 pKa = 3.62QNVEE99 pKa = 4.06ACTGIPVGEE108 pKa = 4.15

Molecular weight: 11.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A366E806|A0A366E806_9RHIZ Uncharacterized protein OS=Pseudochrobactrum asaccharolyticum OX=354351 GN=DFR47_10199 PE=4 SV=1
MM1 pKa = 7.54SRR3 pKa = 11.84ACEE6 pKa = 4.05LTGKK10 pKa = 10.32AVLYY14 pKa = 10.19GNNVSHH20 pKa = 7.36ANNKK24 pKa = 7.61SRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84YY29 pKa = 9.84LPNLCNVTLMSDD41 pKa = 3.83AMGQSYY47 pKa = 10.31RR48 pKa = 11.84LRR50 pKa = 11.84ITAHH54 pKa = 6.56ALRR57 pKa = 11.84SVEE60 pKa = 3.99HH61 pKa = 6.86RR62 pKa = 11.84GGLDD66 pKa = 2.91AFLAKK71 pKa = 10.61SSDD74 pKa = 3.61NEE76 pKa = 3.71LSQRR80 pKa = 11.84ARR82 pKa = 11.84LLKK85 pKa = 10.41RR86 pKa = 11.84QVAKK90 pKa = 10.76KK91 pKa = 9.9LAEE94 pKa = 4.07AAAA97 pKa = 4.16

Molecular weight: 10.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4256

0

4256

1333084

29

3850

313.2

34.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.997 ± 0.047

0.809 ± 0.013

5.601 ± 0.037

5.709 ± 0.037

3.918 ± 0.023

7.826 ± 0.043

2.027 ± 0.02

6.119 ± 0.028

4.346 ± 0.032

9.944 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.677 ± 0.021

3.454 ± 0.026

4.537 ± 0.022

3.747 ± 0.025

5.863 ± 0.039

6.014 ± 0.027

5.623 ± 0.047

6.933 ± 0.034

1.25 ± 0.014

2.605 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski