Escherichia phage Rac

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3AVN6|A0A6M3AVN6_9CAUD Transcriptional regulator OS=Escherichia phage Rac OX=2682812 GN=dicC PE=4 SV=1
MM1 pKa = 7.88ADD3 pKa = 3.67FGSTKK8 pKa = 10.37YY9 pKa = 10.52NVSFEE14 pKa = 4.19EE15 pKa = 3.92WHH17 pKa = 6.24EE18 pKa = 3.83LLMEE22 pKa = 4.14YY23 pKa = 11.02AEE25 pKa = 4.58LRR27 pKa = 11.84SGSAADD33 pKa = 3.53AEE35 pKa = 4.71VWRR38 pKa = 11.84DD39 pKa = 3.32DD40 pKa = 3.82YY41 pKa = 11.69EE42 pKa = 5.41AGKK45 pKa = 8.35TPVEE49 pKa = 4.4AYY51 pKa = 10.04CDD53 pKa = 3.52EE54 pKa = 4.67WGDD57 pKa = 3.64EE58 pKa = 4.11

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3AVP4|A0A6M3AVP4_9CAUD Uncharacterized protein OS=Escherichia phage Rac OX=2682812 PE=4 SV=1
MM1 pKa = 7.38LCIRR5 pKa = 11.84RR6 pKa = 11.84LASYY10 pKa = 9.88RR11 pKa = 11.84PKK13 pKa = 10.56GQGEE17 pKa = 4.3AGYY20 pKa = 9.21EE21 pKa = 3.91AAKK24 pKa = 10.75GDD26 pKa = 3.66VNRR29 pKa = 11.84PDD31 pKa = 2.87RR32 pKa = 11.84HH33 pKa = 5.6LFNRR37 pKa = 11.84RR38 pKa = 11.84CDD40 pKa = 3.44GTGNEE45 pKa = 4.01EE46 pKa = 3.91RR47 pKa = 11.84PLRR50 pKa = 11.84GTNPNRR56 pKa = 11.84PDD58 pKa = 3.39GGRR61 pKa = 11.84CLHH64 pKa = 6.35SLRR67 pKa = 11.84TT68 pKa = 3.77

Molecular weight: 7.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17

0

17

2199

51

262

129.4

14.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.913 ± 0.667

1.637 ± 0.339

4.684 ± 0.437

8.322 ± 0.464

3.547 ± 0.569

6.639 ± 0.51

2.319 ± 0.419

5.866 ± 0.429

6.139 ± 0.319

9.095 ± 0.602

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.729 ± 0.385

4.093 ± 0.389

3.456 ± 0.522

4.457 ± 0.471

7.685 ± 0.526

5.548 ± 0.437

4.638 ± 0.291

5.957 ± 0.609

1.819 ± 0.253

2.456 ± 0.396
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski