Legionella sp. TUM19329

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella; unclassified Legionella

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3144 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6F8T433|A0A6F8T433_9GAMM Glutamate--tRNA ligase OS=Legionella sp. TUM19329 OX=2708020 GN=gltX PE=3 SV=1
MM1 pKa = 6.69VTISNSTISANLVHH15 pKa = 6.91GSFVAGVLNNNSTEE29 pKa = 4.15GLGKK33 pKa = 9.36GTVSITGSTITVNADD48 pKa = 3.18DD49 pKa = 4.94AGLAGGVFNQANNPNGISSNINIDD73 pKa = 3.22QSAISVTSNNNGGGTAAGVLTSGNGTFDD101 pKa = 4.25FNNSSINVAGNSGSIAGVVVGDD123 pKa = 3.95PTATANLQDD132 pKa = 4.21TIISLVTSGTAVGAPIINGGTLNDD156 pKa = 3.86NGGNQCFQNGAPVPCC171 pKa = 5.1

Molecular weight:
16.51 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6F8T2W0|A0A6F8T2W0_9GAMM Tryptophan/tyrosine permease OS=Legionella sp. TUM19329 OX=2708020 GN=TUM19329_08680 PE=4 SV=1
MM1 pKa = 7.16KK2 pKa = 7.76TTSRR6 pKa = 11.84TEE8 pKa = 3.56KK9 pKa = 10.38KK10 pKa = 10.49LYY12 pKa = 10.86SFDD15 pKa = 3.42SAPRR19 pKa = 11.84CGARR23 pKa = 11.84TKK25 pKa = 10.99SNNGEE30 pKa = 4.11PCRR33 pKa = 11.84CPAIRR38 pKa = 11.84GKK40 pKa = 9.57SRR42 pKa = 11.84CRR44 pKa = 11.84VHH46 pKa = 7.16GGARR50 pKa = 11.84GSGAPHH56 pKa = 6.39YY57 pKa = 10.35NLNALKK63 pKa = 10.37HH64 pKa = 5.97GYY66 pKa = 6.18TTGEE70 pKa = 3.54AKK72 pKa = 10.55AFRR75 pKa = 11.84EE76 pKa = 4.47DD77 pKa = 2.58IRR79 pKa = 11.84LAIKK83 pKa = 10.3RR84 pKa = 11.84SNRR87 pKa = 11.84FIKK90 pKa = 10.52EE91 pKa = 3.92LVV93 pKa = 3.23

Molecular weight:
10.35 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3144

0

3144

953758

39

4261

303.4

34.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.483 ± 0.042

1.188 ± 0.017

4.98 ± 0.031

5.962 ± 0.05

4.322 ± 0.033

6.009 ± 0.043

2.512 ± 0.023

7.376 ± 0.042

6.406 ± 0.05

10.873 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.447 ± 0.022

4.992 ± 0.034

3.989 ± 0.027

4.593 ± 0.039

4.293 ± 0.032

6.736 ± 0.031

5.377 ± 0.039

5.954 ± 0.038

1.129 ± 0.017

3.382 ± 0.03

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski