Vibrio nigripulchritudo

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5540 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U4KIP3|U4KIP3_9VIBR 2-amino-3-ketobutyrate coenzyme A ligase OS=Vibrio nigripulchritudo OX=28173 GN=kbl PE=3 SV=1
MM1 pKa = 7.34YY2 pKa = 10.33LPKK5 pKa = 10.27KK6 pKa = 10.51NKK8 pKa = 8.53LTFSDD13 pKa = 3.95LSKK16 pKa = 10.73IIIEE20 pKa = 4.4SNDD23 pKa = 3.24LNLGTFCIYY32 pKa = 7.55TTNSDD37 pKa = 3.77VIISEE42 pKa = 4.14RR43 pKa = 11.84TDD45 pKa = 3.27VYY47 pKa = 10.89IDD49 pKa = 4.6DD50 pKa = 4.56YY51 pKa = 10.86PTGDD55 pKa = 3.94EE56 pKa = 4.23EE57 pKa = 6.35GEE59 pKa = 4.21DD60 pKa = 3.71LFSDD64 pKa = 4.58FVIEE68 pKa = 4.87HH69 pKa = 6.14NLEE72 pKa = 3.75LLYY75 pKa = 11.31YY76 pKa = 9.89GDD78 pKa = 3.65QFVDD82 pKa = 4.01VINNLRR88 pKa = 11.84AQNVPLTIEE97 pKa = 4.35NIIIAANFWLEE108 pKa = 3.49NDD110 pKa = 3.4NFFTFSGG117 pKa = 3.61

Molecular weight: 13.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U4KGD1|U4KGD1_9VIBR Uncharacterized protein OS=Vibrio nigripulchritudo OX=28173 GN=VIBNI_B0429 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPSVLKK12 pKa = 10.49RR13 pKa = 11.84KK14 pKa = 7.97RR15 pKa = 11.84THH17 pKa = 5.89GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.44NGRR29 pKa = 11.84KK30 pKa = 9.39VINARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.22GRR40 pKa = 11.84ARR42 pKa = 11.84LSKK45 pKa = 10.83

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5540

0

5540

1827484

25

4183

329.9

36.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.324 ± 0.033

1.02 ± 0.011

5.482 ± 0.029

6.621 ± 0.033

4.209 ± 0.022

6.879 ± 0.027

2.265 ± 0.019

6.248 ± 0.027

5.389 ± 0.028

10.199 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.018

4.253 ± 0.022

3.95 ± 0.021

4.307 ± 0.023

4.424 ± 0.024

7.023 ± 0.026

5.341 ± 0.035

7.146 ± 0.024

1.287 ± 0.012

3.039 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski