Arsukibacterium sp. MJ3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Arsukibacterium; unclassified Arsukibacterium

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3329 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0M2VJZ6|A0A0M2VJZ6_9GAMM S-adenosylmethionine synthase OS=Arsukibacterium sp. MJ3 OX=1632859 GN=metK PE=3 SV=1
MM1 pKa = 7.54QDD3 pKa = 3.23LEE5 pKa = 4.54LWSMDD10 pKa = 4.08EE11 pKa = 4.37LCDD14 pKa = 3.43HH15 pKa = 6.95AFDD18 pKa = 4.85IFEE21 pKa = 4.62EE22 pKa = 4.29LAADD26 pKa = 3.92NLNAADD32 pKa = 3.88YY33 pKa = 11.23NLYY36 pKa = 8.39QQQYY40 pKa = 7.74EE41 pKa = 3.92QSGYY45 pKa = 10.47VDD47 pKa = 3.3MVIPGAEE54 pKa = 3.87WVEE57 pKa = 3.86LTMQDD62 pKa = 5.13LEE64 pKa = 4.56PEE66 pKa = 4.33LHH68 pKa = 6.8FEE70 pKa = 4.14AQIGLTGSNGAADD83 pKa = 3.68MVLARR88 pKa = 11.84ILLSRR93 pKa = 11.84EE94 pKa = 3.87KK95 pKa = 10.51HH96 pKa = 6.85DD97 pKa = 4.49SLCHH101 pKa = 5.06AQWRR105 pKa = 11.84GQQ107 pKa = 3.21

Molecular weight:
12.25 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0M2VIM7|A0A0M2VIM7_9GAMM Ribosomal RNA small subunit methyltransferase G OS=Arsukibacterium sp. MJ3 OX=1632859 GN=gidB PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPSVLKK12 pKa = 10.46RR13 pKa = 11.84KK14 pKa = 9.37RR15 pKa = 11.84NHH17 pKa = 5.37GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.28SGRR29 pKa = 11.84QVLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.25GRR40 pKa = 11.84QRR42 pKa = 11.84LSAA45 pKa = 4.04

Molecular weight:
5.28 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3329

0

3329

1092290

29

3426

328.1

36.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.219 ± 0.048

0.964 ± 0.015

5.084 ± 0.035

5.056 ± 0.041

4.015 ± 0.029

6.685 ± 0.04

2.215 ± 0.021

5.802 ± 0.033

4.338 ± 0.039

11.665 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.024

4.09 ± 0.033

4.143 ± 0.024

5.919 ± 0.051

4.835 ± 0.03

6.314 ± 0.038

5.267 ± 0.029

6.704 ± 0.038

1.321 ± 0.021

3.019 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski