Flavobacteriaceae bacterium UJ101

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2681 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0LIZ7|A0A1J0LIZ7_9FLAO Putative tRNA/rRNA methyltransferase OS=Flavobacteriaceae bacterium UJ101 OX=1150389 GN=UJ101_00692 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.52KK3 pKa = 10.44LFLTLTVPLYY13 pKa = 10.48LASISCNSDD22 pKa = 3.04DD23 pKa = 5.46DD24 pKa = 4.35NTVSTDD30 pKa = 3.04ITPVLSQTITGIEE43 pKa = 4.28GPEE46 pKa = 3.81SVLLNTNDD54 pKa = 3.16NLLYY58 pKa = 10.66VSNQAGSTDD67 pKa = 3.18GDD69 pKa = 4.29GYY71 pKa = 10.8ISILDD76 pKa = 3.47QSGTVINQKK85 pKa = 9.0WATNLNDD92 pKa = 3.89PKK94 pKa = 11.57GMVIYY99 pKa = 10.05NNKK102 pKa = 9.38MYY104 pKa = 11.05VGDD107 pKa = 3.81TNGLVEE113 pKa = 4.85ISMTDD118 pKa = 3.29GSILQIYY125 pKa = 10.53GEE127 pKa = 4.41GQNLNDD133 pKa = 3.9VAIDD137 pKa = 3.57NDD139 pKa = 3.72GNIYY143 pKa = 9.14VSNMFTSAIYY153 pKa = 10.45QLDD156 pKa = 3.8TNGNFSEE163 pKa = 4.36WLSGSDD169 pKa = 2.98IQNPNGILIDD179 pKa = 4.16GNTMYY184 pKa = 10.82LAPWGTFTGNDD195 pKa = 3.51FASAPPAPVLKK206 pKa = 10.74VDD208 pKa = 4.33LTTKK212 pKa = 10.51AVSQLSKK219 pKa = 8.38TTLGNLDD226 pKa = 4.59GIQFNSTKK234 pKa = 10.0TVLIVSDD241 pKa = 4.59WISGKK246 pKa = 9.93IYY248 pKa = 10.27QVNLSDD254 pKa = 4.7GSSQEE259 pKa = 3.77ILDD262 pKa = 4.03VKK264 pKa = 10.19NTSQTFGGSGDD275 pKa = 3.41ILVNGSKK282 pKa = 10.56LYY284 pKa = 10.61IPMYY288 pKa = 9.9LDD290 pKa = 3.87NSILEE295 pKa = 4.21YY296 pKa = 10.95NII298 pKa = 4.02

Molecular weight: 32.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0LM76|A0A1J0LM76_9FLAO Putative sodium/glutamine symporter GlnT OS=Flavobacteriaceae bacterium UJ101 OX=1150389 GN=UJ101_01534 PE=3 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FKK16 pKa = 11.33VVNNFEE22 pKa = 4.32EE23 pKa = 4.41VTAAKK28 pKa = 9.94PEE30 pKa = 3.84KK31 pKa = 10.61SLVSGKK37 pKa = 10.5KK38 pKa = 8.19KK39 pKa = 9.23TGGRR43 pKa = 11.84NNQGRR48 pKa = 11.84MTNRR52 pKa = 11.84YY53 pKa = 9.04IGGGHH58 pKa = 5.71KK59 pKa = 9.99KK60 pKa = 9.88KK61 pKa = 10.59YY62 pKa = 10.1RR63 pKa = 11.84EE64 pKa = 3.72IDD66 pKa = 3.39FKK68 pKa = 11.16RR69 pKa = 11.84NKK71 pKa = 9.91FGVPATIKK79 pKa = 10.63SIEE82 pKa = 3.97YY83 pKa = 10.23DD84 pKa = 3.5PNRR87 pKa = 11.84SAFIALLYY95 pKa = 9.96YY96 pKa = 10.79ADD98 pKa = 3.85GAKK101 pKa = 9.85TYY103 pKa = 10.95VIAQNGMKK111 pKa = 9.93VGQEE115 pKa = 3.69IVSGEE120 pKa = 3.98NVAPEE125 pKa = 4.28VGNAMPLGAMPLGTIVSCIEE145 pKa = 3.72LRR147 pKa = 11.84PGQGAVMARR156 pKa = 11.84SAGAYY161 pKa = 8.49AQLLARR167 pKa = 11.84EE168 pKa = 4.49GKK170 pKa = 9.36YY171 pKa = 9.1ATVKK175 pKa = 10.52LPSGEE180 pKa = 3.78TRR182 pKa = 11.84MILTTCLATVGVVSNSDD199 pKa = 2.99HH200 pKa = 5.76QLQVSGKK207 pKa = 9.56AGRR210 pKa = 11.84SRR212 pKa = 11.84WKK214 pKa = 10.0GRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84AVVMNPVDD229 pKa = 3.45HH230 pKa = 7.05PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 5.05PRR245 pKa = 11.84DD246 pKa = 3.41RR247 pKa = 11.84KK248 pKa = 10.2GRR250 pKa = 11.84PAKK253 pKa = 10.38GFKK256 pKa = 9.22TRR258 pKa = 11.84AKK260 pKa = 9.94KK261 pKa = 10.05KK262 pKa = 10.29ASNKK266 pKa = 9.97YY267 pKa = 8.74IVEE270 pKa = 4.08RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.24KK274 pKa = 10.1

Molecular weight: 29.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2681

0

2681

933936

29

3961

348.4

39.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.55 ± 0.039

0.724 ± 0.015

5.707 ± 0.075

6.919 ± 0.049

5.082 ± 0.049

6.465 ± 0.053

1.862 ± 0.027

8.474 ± 0.048

8.064 ± 0.062

9.046 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.022

6.276 ± 0.047

3.291 ± 0.028

3.61 ± 0.03

3.212 ± 0.028

6.496 ± 0.043

5.895 ± 0.047

5.868 ± 0.035

1.029 ± 0.016

4.32 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski